An experiment was carried out to determine whether feeding different calcium levels from the onset of production has an effect on medullary bone reserves, plasma proteins and ultimately shell formation. A total of 80 Single Comb White Leghorn hens photostimulated at 17 wk of age were fed a 2.5% Ca diet until the appearance of the first egg. Thereafter diets providing 2.5, 3.0, 3.5, 4.0, or 4.5% Ca were fed to groups of 16 hens. At 6, 12, 18, or 24 h post-ovrposition, blood was collected from four hens per treatment, which were then euthanized and developing shells and bones sampled. As dietary Ca increased, plasma Ca, protein, and protein-bound phosphorus concentrations also increased (P < 0.05). Dietary Ca level had no effect (P > 0.05) on the % Ca or total Ca content of bone ends or medullary bone. As dietary Ca concentration increased, both shell ash and shell Ca increased (P < 0.05). Regardless of dietary Ca, shell secretion was most rapid during 12–18 h of the ovulatory cycle (postoviposition). Key words: Calcium, medullary bone, shell calcification, plasma proteins