Terbium(iii)-cholate functionalized vesicles as luminescent indicators for the enzymatic conversion of dihydroxynaphthalene diesters

The phosphorescence intensity of unilamellar DOPC vesicles with embedded Tb3+-cholate complexes depends on the concentration of dihydroxynaphthalene (DHN) as sensitizer in solution.

Oxygen concentration imaging in a single living cell using phosphorescence lifetime of Pt-porphyrin

An oxygen concentration imaging system inside a single living cell, based on the phosphorescence lifetime, under a microscope was developed. A fluorescence microscope equipped with a pulsed Nd : YAG laser (532 nm) and a CCD camera equipped with a gated imaging intensifier was used. When the cell was incubated with the phosphorescent compound, platinum tetra-(carboxyphenyl)-porphyrin ( PtTCPP ) was incorporated and localized in the cell. As the phosphorescence intensity depends not only on the concentration of a quencher such as oxygen but also on the concentration of phosphorescent molecules, the oxygen concentration was measured by the phosphorescence lifetime. To measure the oxygen concentration image, the time dependence of the phosphorescence intensity was observed under a microscope. The phosphorescence intensity decay obeyed first order kinetics and the oxygen concentration imaging in the single cell was obtained by the lifetime of a single exponential decay curve.

Metalloporphyrins immobilized in styrene–trifluoroethylmethacrylate copolymer film as an optical oxygen sensing probe

A series of new fluoropolymer, poly(styrene-co-trifluoroethylmethacrylate) ( poly - Sty n- co - TFEM m), with different composition ratios of Sty and TFEM units, is synthesized and applied to the matrix of an optical oxygen sensing probe using phosphorescence quenching of metalloporphyrins, platinum and palladium octaethylporphyrin ( PtOEP and PdOEP ), by oxygen. The phosphorescence intensity of PtOEP and PdOEP in poly - Sty n- co - TFEM m film decreased with increase of oxygen concentration. The ratio I 0/ I 100 is used as a sensitivity of the sensing film, where I 0 and I 100 represent the detected phosphorescence intensities from a film exposed to 100% argon and 100% oxygen, respectively. For PtOEP in poly - Sty n- co - TFEM m film, I 0/ I 100 ratios are more than 20.6 and large Stern–Volmer constants greater than 0.49%-1 are obtained compared with PtOEP in PS film. For PdOEP in poly - Sty n- co - TFEM m film, on the other hand, large I 0/ I 100 values greater than 188.7 are obtained. In both cases for PtOEP and PdOEP , I 0/ I 100 values increased with increase of the number of TFEM units in poly - Sty n- co - TFEM m. The response times of PtOEP and PdOEP immobilized in poly - Sty 1- co - TFEM 2.50 films are 5.5 and 3.0 s on going from argon to oxygen and 90.0 and 143 s from oxygen to argon, respectively. These results show that PtOEP and PdOEP immobilized in poly - Sty n- co - TFEM m films are highly sensitive devices for oxygen.