The investigating the gut chapter examines investigations used to diagnosis diseases of the gastrointestinal tract. The investigations need to be used in the appropriate settings as there are risks associated with some, such as the radiation used in scans. Investigating the gut includes the defaecating proctogram to explore the rectum as well as ultrasounds of the abdomen or endoanal. There are also investigations such as computer tomography, virtual colonoscopy, and magnetic resonance imaging. Simple X-rays can be used to examine the abdomen or monitor gut transit, and barium studies can be taken orally or via the anus. These investigations can be used to diagnose changes within the gastrointestinal tract, such as a colorectal cancer or a stricture. Succinct explorations on the different investigations used to examine the gastrointestinal tract, detailed within this chapter can be reviewed when necessary within clinical practice by the nurse.