ACTB Mutations Analysis and Genotype–Phenotype Correlation in Becker’s Nevus

Becker’s nevus (BN) is a cutaneous hamartoma which is characterized by circumscribed hyperpigmentation with hypertrichosis. Recent studies have revealed that BN patients harbored postzygotic ACTB mutations, which were restricted to arrector pili muscle lineage. We screened for ACTB mutations in 20 Chinese patients with BN and found that recurrent mutations (c.C439A or c.C439T) in ACTB were detected in the majority of BN patients. However, more than 20% of the patients were negative for ACTB mutations, suggesting a possible genetic heterogeneity in Becker’s nevus. Interestingly, these mutations were also detected in dermal tissues outside the arrector pili muscle. We further performed genotype–phenotype correlation analysis, which revealed that lesions above the waistline, including the trunk above the anterior superior spine level, upper limbs and face, or covering more than 1% BSA were more likely to be positive for ACTB mutations. Altogether, our results provide further evidence of postzygotic ACTB mutations in BN patients and suggest a possible genotype–phenotype correlation of BN.

INTRODUCING THEORY LINKING GENESIS OF HAIR SHAFT BIPOLARITY WITH ARRECTOR PILI MUSCLE PROXIMAL ATTACHMENT

Previous publications described the presence of bipolarity or (+−) electrical charges in the human hair shaft, this due to an apparent segmental electromagnetic radiation Gap demonstrated by a total absence of precipitated Potassium Ferricyanide crystals. In this manuscript additional data is presented elucidating the genesis of said absence. The in vitro experiments showing a correlation between a segmental absence of longitudinal terminal palisades nervous endings prevalent in the rest of the outer membrane in the hair follicle anatomy. Due to their intrinsic metabolism, nerve cells do emit electromagnetic radiation; their absence not allowing for the positive (+) charge of zero precipitated Potassium Ferricyanide crystals to reach one side of the shaft. In addition it was concluded that there are two types of human hair images. The first by optical microscopy, the second a functional one obtained from electromagnetic radiation precipitating Ferricyanide crystals.