surface assembly
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Author(s):  
Brandy Perkins-Howard ◽  
Ashley R. Walker ◽  
Quynh Do ◽  
Dodangodage Ishara Senadheera ◽  
Fawwaz Hazzazi ◽  
...  

2021 ◽  
pp. 113268
Author(s):  
Han-Wen Cheng ◽  
Shan Yan ◽  
Guojun Shang ◽  
Shan Wang ◽  
Chuan-Jian Zhong

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 299
Author(s):  
Silvio Osella

A tremendous effort is currently devoted to the generation of novel hybrid materials with enhanced electronic properties for the creation of artificial photosynthetic systems. This compelling and challenging problem is well-defined from an experimental point of view, as the design of such materials relies on combining organic materials or metals with biological systems like light harvesting and redox-active proteins. Such hybrid systems can be used, e.g., as bio-sensors, bio-fuel cells, biohybrid photoelectrochemical cells, and nanostructured photoelectronic devices. Despite these efforts, the main bottleneck is the formation of efficient interfaces between the biological and the organic/metal counterparts for efficient electron transfer (ET). It is within this aspect that computation can make the difference and improve the current understanding of the mechanisms underneath the interface formation and the charge transfer efficiency. Yet, the systems considered (i.e., light harvesting protein, self-assembly monolayer and surface assembly) are more and more complex, reaching (and often passing) the limit of current computation power. In this review, recent developments in computational methods for studying complex interfaces for artificial photosynthesis will be provided and selected cases discussed, to assess the inherent ability of computation to leave a mark in this field of research.


2021 ◽  
Vol 57 (12) ◽  
pp. 1454-1457
Author(s):  
Roelof Steeno ◽  
Andrea Minoia ◽  
Maria C. Gimenez-Lopez ◽  
Matthew O. Blunt ◽  
Neil R. Champness ◽  
...  

The presence of an opportune impurity can change the on-surface assembly behavior via preferential adsorption and nucleation.


2020 ◽  
Vol 46 (11) ◽  
pp. 19499-19505 ◽  
Author(s):  
Abdul Jabbar Khan ◽  
Asad Khan ◽  
Muhammad Sufyan Javed ◽  
Muhammad Arshad ◽  
Sumreen Asim ◽  
...  

2020 ◽  
Author(s):  
Ce Dong ◽  
Jie Qiao ◽  
Xinping Wang ◽  
Wenli Sun ◽  
Lixia Chen ◽  
...  

Abstract Backgrounds: Engineering yeast as a consolidated bioprocessing (CBP) microorganism by surface assembly of cellulosomes has been aggressively utilized for cellulosic ethanol production. However, most of the previous studies focused on Saccharomyces cerevisiae, achieving efficient conversion of phosphoric acid-swollen cellulose (PASC) or microcrystalline cellulose (Avicel) but not carboxymethyl cellulose (CMC) to ethanol, with an average titer below 2 g/L. Results: Harnessing an ultra-high-affinity IM7/CL7 protein pair, here we describe a method to engineer Pichia pastoris with minicellulosomes by in vitro assembly of three recombinant cellulases including an endoglucanase (EG), an exoglucanase (CBH) and a β-glucosidase (BGL), as well as a carbohydrate binding module (CBM) on the cell surface. For the first time, the engineered yeasts enable efficient and direct conversion of CMC to bioethanol, observing an impressive ethanol titer of 5.1 g/L. Conclusions: The research promotes the application of P. pastoris as a CBP cell factory in cellulosic ethanol production and provides a promising platform for screening the cellulases from different species to construct surface-assembly celluosome.


2020 ◽  
Vol 124 (23) ◽  
pp. 12502-12511 ◽  
Author(s):  
Kerstin Oppelt ◽  
Mathias Mosberger ◽  
Jeannette Ruf ◽  
Ricardo Fernández-Terán ◽  
Benjamin Probst ◽  
...  

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