Optimization of Protoplast Formation, Regeneration, and Viability in Sorangium cellulosum

Sorangium cellulosum can product many secondary metabolites that is unique structural and makes these microorganisms highly attractive for drug development, especially epothilone, on cancer cells a cytotoxic macrolide which is naturally produced by Soxhlet cellulose that have the action of microtubule stabilization, is a promising anticancer drug. In this research, the factors affecting the regeneration and preparation of the protoplast of Sorangium cellulosum were discussed, those were regeneration media, enzymes and osmotic stabilizers. This study provide the distruction for improving the production of epothilone through genome shuffling, mutation, fusion and transformation.

Enantioselective formal synthesis of the marine macrolide (−)-callyspongiolide

An enantioselective formal synthesis of the cytotoxic macrolide (−)-callyspongiolide, in eight steps from aldehyde 4, is reported.

Total Synthesis of Biselide A, A Cytotoxic Macrolide of Marine Origin

The total synthesis of biselide A based on our earlier strategy of synthesizing haterumalides is reported. The highlights of this approach are the use of regioselective enzymatic hydrolysis for the installation of a C20 oxygen functional group and an asymmetric aldol reaction for the stereoselective introduction of a C3 oxygen functional group.