Optimization of Protoplast Formation, Regeneration, and Viability in Sorangium cellulosum

Sorangium cellulosum can product many secondary metabolites that is unique structural and makes these microorganisms highly attractive for drug development, especially epothilone, on cancer cells a cytotoxic macrolide which is naturally produced by Soxhlet cellulose that have the action of microtubule stabilization, is a promising anticancer drug. In this research, the factors affecting the regeneration and preparation of the protoplast of Sorangium cellulosum were discussed, those were regeneration media, enzymes and osmotic stabilizers. This study provide the distruction for improving the production of epothilone through genome shuffling, mutation, fusion and transformation.

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Interaction of Alkylphospholipid Formulations with Breast Cancer Cells in the Context of Anticancer Drug Development

Breast Cancer - Focusing Tumor Microenvironment, Stem cells and Metastasis ◽

10.5772/20584 ◽

2011 ◽

Author(s):

Tilen Koklic ◽

Rok Podlipec ◽

Janez Mravljak ◽

Marjeta Sentjurc ◽

Reiner Zeisig

Keyword(s):

Breast Cancer ◽

Drug Development ◽

Cancer Cells ◽

Anticancer Drug ◽

Breast Cancer Cells ◽

Anticancer Drug Development

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Differential Cytostatic and Cytotoxic Action of Metallocorroles against Human Cancer Cells: Potential Platforms for Anticancer Drug Development

Chemical Research in Toxicology ◽

10.1021/tx200452w ◽

2012 ◽

Vol 25 (2) ◽

pp. 400-409 ◽

Cited By ~ 49

Author(s):

Punnajit Lim ◽

Atif Mahammed ◽

Zoya Okun ◽

Irena Saltsman ◽

Zeev Gross ◽

...

Keyword(s):

Drug Development ◽

Cancer Cells ◽

Anticancer Drug ◽

Human Cancer ◽

Cytotoxic Action ◽

Human Cancer Cells ◽

Anticancer Drug Development

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Telomerase inhibition enhances the response to anticancer drug treatment in human breast cancer cells

Molecular Cancer Therapeutics ◽

10.1158/1535-7163.mct-06-0033 ◽

2006 ◽

Vol 5 (7) ◽

pp. 1669-1675 ◽

Cited By ~ 41

Author(s):

Maria Antonietta Cerone ◽

J. Arturo Londoño-Vallejo ◽

Chantal Autexier

Keyword(s):

Breast Cancer ◽

Drug Treatment ◽

Cancer Cells ◽

Anticancer Drug ◽

Human Breast Cancer ◽

Breast Cancer Cells ◽

Human Breast Cancer Cells ◽

Human Breast ◽

Telomerase Inhibition

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Use of Half-Generation PAMAM Dendrimers (G0.5–G3.5) with Carboxylate End-Groups to Improve the DACHPtCl2 and 5-FU Efficacy as Anticancer Drugs

Molecules ◽

10.3390/molecules26102924 ◽

2021 ◽

Vol 26 (10) ◽

pp. 2924

Author(s):

Cláudia Camacho ◽

Helena Tomás ◽

João Rodrigues

Keyword(s):

Cancer Cells ◽

Cell Lines ◽

Cancer Cell ◽

Anticancer Drug ◽

Water Solubility ◽

Cancer Cell Lines ◽

Pamam Dendrimers ◽

Binding Assays ◽

End Groups ◽

Ic50 Values

The DACHPtCl2 compound (trans-(R,R)-1,2-diaminocyclohexanedichloroplatinum(II)) is a potent anticancer drug with a broad spectrum of activity and is less toxic than oxaliplatin (trans-l-diaminocyclohexane oxalate platinum II), with which it shares the active metal fragment DACHPt. Nevertheless, due to poor water solubility, its use as a chemotherapeutic drug is limited. Here, DACHPtCl2 was conjugated, in a bidentate form, with half-generation PAMAM dendrimers (G0.5–G3.5) with carboxylate end-groups, and the resulting conjugates were evaluated against various types of cancer cell lines. In this way, we aimed at increasing the solubility and availability at the target site of DACHPt while potentially reducing the adverse side effects. DNA binding assays showed a hyperchromic effect compatible with DNA helix’s disruption upon the interaction of the metallodendrimers and/or the released active metallic fragments with DNA. Furthermore, the prepared DACHPt metallodendrimers presented cytotoxicity in a wide set of cancer cell lines used (the relative potency regarding oxaliplatin was in general high) and were not hemotoxic. Importantly, their selectivity for A2780 and CACO-2 cancer cells with respect to non-cancer cells was particularly high. Subsequently, the anticancer drug 5-FU was loaded in a selected metallodendrimer (the G2.5COO(DACHPt)16) to investigate a possible synergistic effect between the two drugs carried by the same dendrimer scaffold and tested for cytotoxicity in A2780cisR and CACO-2 cancer cell lines. This combination resulted in IC50 values much lower than the IC50 for 5-FU but higher than those found for the metallodendrimers without 5-FU. It seems, thus, that the metallic fragment-induced cytotoxicity dominates over the cytotoxicity of 5-FU in the set of considered cell lines.

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