Eavesdropping on Referential Yellow Warbler Alarm Calls by Red-Winged Blackbirds Is Mediated by Brood Parasitism Risk

Referential alarm calls that denote specific types of dangers are common across diverse vertebrate lineages. Different alarm calls can indicate a variety of threats, which often require specific actions to evade. Thus, to benefit from the call, listeners of referential alarm calls must be able to decode the signaled threat and respond to it in an appropriate manner. Yellow warblers (Setophaga petechia) produce referential “seet” calls that signal to conspecifics the presence of nearby obligate brood parasitic brown-headed cowbirds (Molothrus ater), which lay their eggs in the nests of other species, including yellow warblers. Our previous playback experiments have found that red-winged blackbirds (Agelaius phoeniceus), a species also parasitized by brown-headed cowbirds, eavesdrop upon and respond strongly to yellow warbler seet calls during the incubation stage of breeding with aggression similar to responses to both cowbird chatters and predator calls. To assess whether red-winged blackbird responses to seet calls vary with their own risk of brood parasitism, we presented the same playbacks during the nestling stage of breeding (when the risk of brood parasitism is lower than during incubation). As predicted, we found that blackbirds mediated their aggression toward both cowbird chatter calls and the warblers’ anti-parasitic referential alarm calls in parallel with the low current risk of brood parasitism during the nestling stage. These results further support that red-winged blackbirds flexibly respond to yellow warbler antiparasitic referential calls as a frontline defense against brood parasitism at their own nests.

Referential alarm calling elicits future vigilance in a host of an avian brood parasite

Yellow warblers ( Setophaga petechia ) use referential ‘seet’ calls to warn mates of brood parasitic brown-headed cowbirds ( Molothrus ater ). In response to seet calls during the day, female warblers swiftly move to sit tightly on their nests, which may prevent parasitism by physically blocking female cowbirds from inspecting and laying in the nest. However, cowbirds lay their eggs just prior to sunrise, not during daytime. We experimentally tested whether female warblers, warned by seet calls on one day, extend their anti-parasitic responses into the future by engaging in vigilance at sunrise on the next day, when parasitism may occur. As predicted, daytime seet call playbacks caused female warblers to leave their nests less often on the following morning, relative to playbacks of both their generic anti-predator calls and silent controls. Thus, referential calls do not only convey the identity or the type of threat at present but also elicit vigilance in the future to provide protection from threats during periods of heightened vulnerability.

Post-glacial expansion dynamics, not refugial isolation, shaped the genetic structure of a migratory bird, the Yellow Warbler (Setophaga petechia)

During the glacial periods of the Pleistocene, swathes of the Northern Hemisphere were covered by ice sheets, tundra and permafrost leaving large areas uninhabitable for temperate and boreal species. The glacial refugia paradigm proposes that, during glaciations, species living in the Northern Hemisphere were forced southwards, forming isolated, insular populations that persisted in disjunct regions known as refugia. According to this hypothesis, as ice sheets retreated, species recolonised the continent from these glacial refugia, and the mixing of these lineages is responsible for modern patterns of genetic diversity. However, an alternative hypothesis is that complex genetic patterns could also arise simply from heterogenous post-glacial expansion dynamics, without separate refugia. Both mitochondrial and genomic data from the North American Yellow warbler (Setophaga petechia) shows the presence of an eastern and western clade, a pattern often ascribed to the presence of two refugia. Using a climate-informed spatial genetic modelling (CISGeM) framework, we were able to reconstruct past population sizes, range expansions, and likely recolonisation dynamics of this species, generating spatially and temporally explicit demographic reconstructions. The model captures the empirical genetic structure despite including only a single, large glacial refugium. The contemporary population structure observed in the data was generated during the expansion dynamics after the glaciation and is due to unbalanced rates of northward advance to the east and west linked to the melting of the icesheets. Thus, modern population structure in this species is consistent with expansion dynamics, and refugial isolation is not required to explain it, highlighting the importance of explicitly testing drivers of geographic structure.

Comparison of prey availability for Setophaga petechia gundlachi (Aves: Parulidae) between two mangroves with different vegetation structure

The subspecies Setophaga petechia gundlachi is characterized by its specificity to mangrove habitat during the breeding season. The objective of this work was to compare the prey availability for S. petechia gundlachi during the breeding season in two mangrove sites with different vegetation structure in Havana, Cuba. To describe different levels of vegetation structure between both mangrove sites, we took several variables at 50 plots. Besides, we recorded the foraging behavior of S. petechia gundlachi to establish the design of prey availability sampling. We measured prey availability by using the branch clipping method, about 90 samples were collected at each mangrove site. Bajo de Santa Ana mangrove, with a lower height and diameter at breast height, showed evidence of deforestation. Laguncularia racemosa was the most used mangrove species for foraging and offered the highest biomass of available prey in the Laguna de Cobre-Itabo mangrove site. The greatest biomass of available prey was found at the Laguna de Cobre-Itabo, with a value of 5.1 (CI: 4.0 - 6.2) mg / 100 g of branch clipping. The Bajo de Santa Ana site had a lower value than expected 1.6 (CI: 0.8 - 1.7) mg / 100 g of branch clipping. The results provide evidence of the possible influence of mangrove vegetation structure changes on food availability for S. petechia gundlachi in this urban landscape.