Collective cell behavior is essential for tissue growth, development and function, e.g. heartbeat1, immune responses2 and cerebral consciousness3. In recent years, studies on population cells uncover that collective behavior emerges in both inter- and intra-cellular activities, e.g. synchronized signal cascade4, and collective migration5. As the movement and shape transition of cells within the crowded environment of biological tissue can generate mechanical cues at the cell-cell interface, which may affect the signaling cascade6,7, we suspect that the inter- and intra-cellular collective behavior interplay with one another and cooperatively regulate life machinery. To verify our hypothesis, we study the collective responses of fibroblasts in a confluent cell monolayer (CCM). Our results demonstrate that cells in CCM show distinctive behavior as compared to the stand-alone (SA) cells, suggesting effect of inter-cellular interactions. Upon periodic TNF-α stimulation, collective behavior emerges simultaneously in NF-κB signaling cascade and nuclear shape fluctuations in CCM but not SA cells. We then model the inter-cellular interactions in CCM using a customized microfluidic device, and discover a feedback loop intrinsic to CCM, in which dynamic mechanical cues and mechano-signaling act as link connecting the inter- and intra-cellular collective activities. We found that mechano-signaling triggered by the dynamic mechanical cues causes collective nuclear shape fluctuation (NSF), which subsequently facilitates the collective behavior in NF-κB dynamics. Furthermore, our studies reveal that regardless of the input TNF-α periodicity, cellular responses of single fibroblasts are elevated when the dynamic mechanical cues synergize with the chemical inputs, and inhibited when there is phase-mismatching. We, therefore, postulate that besides the biological significance of mechano-signaling in regulating collective cell responses, the induction of dynamic mechanical cues to human body may be a potential therapeutic approach, allowing us to regulate the action of single cells to achieve optimal tissue performance.