Witnesses to crimes are sometimes reluctant to identify the culprit in a lineup (e.g., for fear of retribution). We introduce an ERP-based guilty knowledge test for sequential lineup identifications, using an oddball paradigm to evoke the P300 component when a witness sees a photo of a culprit compared to those evoked by an innocent familiar face. At the group level, clear differences were found between P300 amplitudes evoked by the culprit’s face and the innocent filler face. At the individual level, the participants’ waveforms were less diagnostic. This method of eyewitness assessment may prove useful if the procedure can be improved in ways that clarify P300 amplitudes for individual participants. Success in this endeavor would be best applied with witnesses who recognize the culprit easily but are compelled to claim falsely that they do not.