Differential diagnostics of the presence of damage by the method of digital polarization mapping of microsocopy images of hystological sections of human organs

The article presents the results of experimental testing of methods of azimuthal-invariant polarization mapping of microscopic images of samples of histological sections of the myocardium and lung tissue; time monitoring of changes in the magnitude of statistical moments of the 1-4th orders, characterizing the distributions of the azimuth and ellipticity of polarization of microscopic images of myocardial and lung tissue samples with different age of damage; determination of the diagnostic efficiency (time interval and accuracy) of establishing the age of damage to human internal organs by digital histological methods of mapping azimuth maps and polarization ellipticity of microscopic images. Aim of the work. Development of a digital histology technique for samples of human internal organs. Materials and methods. The object of the study was the histology of samples of human internal organs (myocardium, lung tissue) with different duration of damage from 1 hour to 120 hours. For control, we used BT samples from those who died from coronary artery disease with different duration of damage from 1 hour to 120 hours. The studies were carried out using the method of digital polarization mapping of microscopic images of histological sections of biological tissues of human internal organs. Results. Revealed the following ranges ramp variation quantity statistics polarizing digital histology and accuracy of the limitation of damage: a. Arts azimuth polarization microscopic image of a magnification ×40 (asymmetry – 12 hours, kurtosis – 12 hours, accuracy – 55-60 min), maps of ellipticity of polarization of microscopic images with a magnification of ×40 (asymmetry – 12 hours, kurtosis – 12 hours, accuracy – 65-75 min). Conclusions. Scenarios of changes in the statistical structure of maps of azimuth and ellipticity of polarization of microscopic images of histological sections of human internal organs have been determined - with an increase in the duration of damage, the value of the mean and variance decreases, the asymmetry and kurtosis increase.

CLASSIFICATION OF MULTIDIMENSIONAL POLARIZATION MICROSCOPY RESULTS IN THE TECHNOLOGY OF FORENSIC INTELLECTUAL MONITORING OF HEART DISEASES

The work combines methods of multidimensional polarization microscopy, statistical processing of data and inductive modeling with the purpose of constructing a methodology for creation of intelligent systems for multi-level forensic medical monitoring based on the example of the post-mortem diagnosis of coronary heart disease and acute coronary insufficiency. The task of classifying the results of the study of biological materials for obtaining a diagnosis was solved. To obtain informative features, a model of biological tissue of the myocardium was developed and the main diagnostic parameters were determined (statistical moments of 1–4 orders of coordinate distributions of the values of azimuths and the ellipticity of polarization and their autocorrelation functions, as well as wavelet coefficients of the corresponding distributions), which are dynamic due to its necrotic changes. The classification of these data was provided by constructing a deciding rule in the multi – raw algorithm of the GMDH. The effectiveness of the described methodology has been experimentally proved.

INFORMATIVELY OF HISTOLOGICAL STUDY BY METHOD OF MAPPING ELLIPTICITY OF POLARIZATION OF MICROSCOPIC MYOCARDIAL IMAGES

Purpose of the study. To investigate the informational value of the histological examination of the human myocardium in case of sudden cardiac death and the possibility of a method of mapping ellipticity of polarization of microscopic images of the myocardium in the diagnosis and objective differentiation of acute coronary insufficiency (ACI) and chronic coronary heart disease (CIHD). Materials and methods. Samples of human myocardium from 89 people who died as a result of ACI and CIHD were investigated, using traditional histological and laser polarization techniques, with a standard-scheme laser polarimeter. Subsequently, statistical processing of the obtained results was conducted. Results. The results obtained show that the values of the statistical moments of the 3rd and 4th order, which characterize the coordinate distributions of the ellipticity of polarization of microscopic images of myocardial samples on the scale of optical magnification ×56, were the most informative for differential diagnosis of ACI and CIHD. Accuracy of ACI and CIHD detection by mapping the ellipticity of polarization of myocardial microscopic images at an optical magnification of ×56 reaches ~ 97%, as opposed to the traditional histological examination ~ 81%. Conclusion. On the basis of the obtained data, we confirm the diagnostic efficiency and accuracy of the method of mapping the values of ellipticity polarization of microscopic images of histological sections of the myocardium in the problem of diagnosis and objective differentiation of ACI and CIHD. Keywords: laser polarimetry, histology, myocardium, sudden cardiac death.

СТАТИСТИЧНИЙ АНАЛІЗ ЛАЗЕРНИХ ПОЛЯРИЗАЦІЙНИХ ЗОБРАЖЕНЬ ПЛАЗМИ КРОВІ В ДІАГНОСТИЦІ ПАТОЛОГІЇ МОЛОЧНИХ ЗАЛОЗ

There are changes tendentious statistical moments of two-dimensional distribution of azimuth and ellipticity of polarization of a laser image of human blood plasma. Evaluation was carried out under the assumption that small and high level of birefringence reflects the state plasma "healthy person" and «pathology of mammary glands».