scholarly journals Differential diagnostics of the presence of damage by the method of digital polarization mapping of microsocopy images of hystological sections of human organs

2021 ◽  
pp. 70-78
Author(s):  
Oleksandra Litvinenko ◽  
Oleh Vanchulyak ◽  
Irina Soltys ◽  
Olga Mikhailova ◽  
Artem Motrich
Keyword(s):  
Lung Tissue ◽  
Experimental Testing ◽  
Biological Tissues ◽  
Differential Diagnostics ◽  
Time Interval ◽  
Diagnostic Efficiency ◽  
Internal Organs ◽  
Microscopic Images ◽  
Histological Sections ◽  
Ellipticity Of Polarization

The article presents the results of experimental testing of methods of azimuthal-invariant polarization mapping of microscopic images of samples of histological sections of the myocardium and lung tissue; time monitoring of changes in the magnitude of statistical moments of the 1-4th orders, characterizing the distributions of the azimuth and ellipticity of polarization of microscopic images of myocardial and lung tissue samples with different age of damage; determination of the diagnostic efficiency (time interval and accuracy) of establishing the age of damage to human internal organs by digital histological methods of mapping azimuth maps and polarization ellipticity of microscopic images. Aim of the work. Development of a digital histology technique for samples of human internal organs. Materials and methods. The object of the study was the histology of samples of human internal organs (myocardium, lung tissue) with different duration of damage from 1 hour to 120 hours. For control, we used BT samples from those who died from coronary artery disease with different duration of damage from 1 hour to 120 hours. The studies were carried out using the method of digital polarization mapping of microscopic images of histological sections of biological tissues of human internal organs. Results. Revealed the following ranges ramp variation quantity statistics polarizing digital histology and accuracy of the limitation of damage: a. Arts azimuth polarization microscopic image of a magnification ×40 (asymmetry – 12 hours, kurtosis – 12 hours, accuracy – 55-60 min), maps of ellipticity of polarization of microscopic images with a magnification of ×40 (asymmetry – 12 hours, kurtosis – 12 hours, accuracy – 65-75 min). Conclusions. Scenarios of changes in the statistical structure of maps of azimuth and ellipticity of polarization of microscopic images of histological sections of human internal organs have been determined - with an increase in the duration of damage, the value of the mean and variance decreases, the asymmetry and kurtosis increase.

scholarly journals Differential Mueller matrix imaging of partially depolarizing optically anisotropic biological tissues

2019 ◽  
Vol 35 (4) ◽  
pp. 877-891 ◽  
Author(s):  
L. Trifonyuk ◽  
A. Sdobnov ◽  
W. Baranowski ◽  
V. Ushenko ◽  
O. Olar ◽  
...  
Keyword(s):  
Biological Tissues ◽  
Mueller Matrix ◽  
Differential Diagnostics ◽  
Matrix Elements ◽  
Polycrystalline Structure ◽  
First Order ◽  
Histological Sections ◽  
Laser Polarimetry ◽  
Matrix Mapping ◽  
Differential Matrix

AbstractSince recently, a number of innovative polarization-based optical imaging modalities have been introduced and extensively used in various biomedical applications, with an ultimate aim to attain the practical tool for the optical biopsy and functional characterization of biological tissues. The techniques utilize polarization properties of light and Mueller matrix mapping of microscopic images of histological sections of biological tissues or polycrystalline films of biological fluids. The main drawback of currently developed laser polarimetry approaches and Mueller matrix mapping techniques is poor reproducibility of experimental data. This is due to azimuthal dependence of polarization and ellipticity values of most matrix elements to sample orientation in respect to incidence light polarization. Current study aims to generalize the methods of laser polarimetry for diagnosis of partially depolarizing optically anisotropic biological tissues. A method of differential Mueller matrix mapping for reconstruction of linear and circular birefringence and dichroism parameter distributions of partially depolarizing layers of biological tissues of different morphological structure is introduced and practically implemented. The coordinate distributions of the value of the first-order differential matrix elements of histological sections of brain tissue with spatially structured, optically anisotropic fibrillar network, as well as of parenchymatous tissue of the rectum wall with an “islet” polycrystalline structure are determined. Within the statistical analysis of polarization reproduced distributions of the averaged parameters of phase and amplitude anisotropy, the significant sensitivity of the statistical moments of the third and fourth orders to changes in the polycrystalline structure of partially depolarizing layers of biological tissue is observed. The differentiation of female reproductive sphere connective tissue is realized with excellent accuracy. The differential Mueller matrix mapping method for reconstruction of distributions of linear and circular birefringence and dichroism parameters of partially depolarizing layers of biological tissues of different morphological structures is proposed and substantiated. Differential diagnostics of changes in the phase (good balanced accuracy) and amplitude (excellent balanced accuracy) of the anisotropy of the partially depolarizing layers of the vagina wall tissue with prolapse of the genitals is realized. The maximum diagnostic efficiency of the first-order differential matrix method was demonstrated in comparison with the traditional methods of polarization and Mueller matrix mapping of histological sections of light-scattering biological tissues.

scholarly journals ESTIMATION OF THE TIME SINCE DEATH BY USING THE POLARIZATION MULLER-MATRIX MICROSCOPY OF BIOLOGICAL TISSUE IN CASES OF ALCOHOL AND CARBON MONOXIDE POISONING

2020 ◽  
Vol 1 ◽  
pp. 41-47
Author(s):  
Ihor Ivaskevich ◽  
Oleh Vanchuliak ◽  
Viktor Bachynskіy ◽  
Sviatoslava Yurniuk
Keyword(s):  
Carbon Monoxide ◽  
Adrenal Glands ◽  
Carbon Monoxide Poisoning ◽  
Biological Tissues ◽  
Time Since Death ◽  
Diagnostic Efficiency ◽  
Histological Sections ◽  
Sensitivity Range ◽  
Diagnostic Capabilities ◽  
The Brain

Introduction: This paper aims to investigate and substantiate the information capabilities of the objective method of azimuthal-invariant polarization Müller-matrix microscopy of histological sections of biological tissues (BT) in the diagnosis of time since death (TSD) in alcohol and monoxide poisoning. Objectives: The purpose of the study was to evaluate the diagnostic capabilities of the azimuthal-invariant Muller-matrix polarimetry technique of the polycrystalline constituent of BT and human body fluids in determining the TSD in cases of alcohol and carbon monoxide poisoning. Results: A comprehensive experimental study of the diagnostic efficiency of the azimuthal-invariant Muller-matrix polarimetric microscopy of the polycrystalline component of histological sections of the brain, myocardium, adrenal glands, liver, and polycrystalline blood films from dead persons for the diagnosis of TSD in cases of alcohol and carbon monoxide poisoning.A sensitivity range of 24 hours was determined using the method of the azimuthal-invariant Muller-matrix polarimetric microscopy of the polycrystalline component of histological sections of the myocardium for the diagnosis of TSD in cases of alcohol and carbon monoxide poisoning. Conclusion: The results showed the effectiveness of the studied methodology and the prospect of further research in this direction.

scholarly journals INFORMATIVELY OF HISTOLOGICAL STUDY BY METHOD OF MAPPING ELLIPTICITY OF POLARIZATION OF MICROSCOPIC MYOCARDIAL IMAGES

2019 ◽  
Vol 43 (4) ◽  
pp. 38-42
Author(s):  
O.YA. Vanchulyak ◽  
O. YU. Lytvynenko ◽  
O. H. Ushenko ◽  
A. O. Andriychuk
Keyword(s):  
Sudden Cardiac Death ◽  
Histological Examination ◽  
Cardiac Death ◽  
Histological Study ◽  
Human Myocardium ◽  
Diagnostic Efficiency ◽  
Coronary Insufficiency ◽  
Microscopic Images ◽  
Optical Magnification ◽  
Ellipticity Of Polarization

Abstract Purpose of the study. To investigate the informational value of the histological examination of the human myocardium in case of sudden cardiac death and the possibility of a method of mapping ellipticity of polarization of microscopic images of the myocardium in the diagnosis and objective differentiation of acute coronary insufficiency (ACI) and chronic coronary heart disease (CIHD). Materials and methods. Samples of human myocardium from 89 people who died as a result of ACI and CIHD were investigated, using traditional histological and laser polarization techniques, with a standard-scheme laser polarimeter. Subsequently, statistical processing of the obtained results was conducted. Results. The results obtained show that the values of the statistical moments of the 3rd and 4th order, which characterize the coordinate distributions of the ellipticity of polarization of microscopic images of myocardial samples on the scale of optical magnification ×56, were the most informative for differential diagnosis of ACI and CIHD. Accuracy of ACI and CIHD detection by mapping the ellipticity of polarization of myocardial microscopic images at an optical magnification of ×56 reaches ~ 97%, as opposed to the traditional histological examination ~ 81%. Conclusion. On the basis of the obtained data, we confirm the diagnostic efficiency and accuracy of the method of mapping the values of ellipticity polarization of microscopic images of histological sections of the myocardium in the problem of diagnosis and objective differentiation of ACI and CIHD. Keywords: laser polarimetry, histology, myocardium, sudden cardiac death.

scholarly journals MAPPING OF AZIMUTHS OF POLARIZATION OF LASER MICROSCOPIC IMAGES OF HISTOLOGICAL SECTIONS OF HUMAN RGANS IN THE DIFFERENTIATION OF POISONING BY ETHANOL AND CARBON MONOXIDE

2019 ◽  
Vol 43 (4) ◽  
pp. 70-74
Author(s):  
I.B. Ivaskevych ◽  
V. T. Bachynskyy ◽  
O. Y. Vanchulyak ◽  
O. H. Palyvoda
Keyword(s):  
Carbon Monoxide ◽  
Adrenal Glands ◽  
Statistical Processing ◽  
Biological Tissues ◽  
Statistical Moments ◽  
Co Poisoning ◽  
Microscopic Images ◽  
Histological Sections ◽  
The Brain ◽  
Keywords Laser

Abstract Purpose of the study. This work is aimed at research and substantiation of information possibilities of objective method of polarization mapping of microscopic images of histological sections of human biological tissues for the establishment and differentiation of С2Н5ОН and CO poisoning. Materisls and methods. Native histological sections of the brain, myocardium and adrenal glands from ethanol poisoning (n = 30) and CO (n = 20) were investigated using a laser polarimeter of standard scheme. Subsequently, statistical processing of the obtained results was conducted. Results. Statistical moments of 1–4th order were determined by the method of polarization mapping of microscopic images of histological sections of human biological tissues. The values of the statistical moments of the 1–4th order for brain tissue for С2Н5ОН and CO poisoning reach differences by 2–2,5 times, for myocardial tissue – 2,2–2,9 times, the adrenal glands – 1,4–1,8 times. Accordingly, the statistical moments of the 3rd and 4th order are more sensitive to the detection and differentiation of poisons, whereby the accuracy of the method of differential diagnosis of poisoning С2Н5ОН and CO reaches an adequate level (~ 90%) in the study of histological sections of the myocardium and brain. Conclusion. The obtained results show that the method of polarization mapping of microscopic images of human biological tissues gives an objective possibility of reliable establishment and differentiation of С2Н5ОН and СО poisoning. Keywords: laser polarimetry, poisoning, ethanol, carbon monoxide, forensic medicine.

scholarly journals Spatial-Frequency Azimuthally Stable Cartography of Biological Polycrystalline Networks

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
V. A. Ushenko ◽  
N. D. Pavlyukovich ◽  
L. Trifonyuk
Keyword(s):  
Spatial Frequency ◽  
Optical Activity ◽  
Polarization Plane ◽  
Biological Tissues ◽  
Uterine Wall ◽  
Beam Polarization ◽  
Linear Birefringence ◽  
Histological Sections ◽  
The Matrix ◽  
Anisotropic Structures

A new azimuthally stable polarimetric technique processing microscopic images of optically anisotropic structures of biological tissues histological sections is proposed. It has been used as a generalized model of phase anisotropy definition of biological tissues by using superposition of Mueller matrices of linear birefringence and optical activity. The matrix elementM44has been chosen as the main information parameter, whose value is independent of the rotation angle of both sample and probing beam polarization plane. For the first time, the technique of concerted spatial-frequency filtration has been used in order to separate the manifestation of linear birefringence and optical activity. Thereupon, the method of azimuthally stable spatial-frequency cartography of biological tissues histological sections has been elaborated. As the analyzing tool, complex statistic, correlation, and fractal analysis of coordinate distributions ofM44element has been performed. The possibility of using the biopsy of the uterine wall tissue in order to differentiate benign (fibromyoma) and malignant (adenocarcinoma) conditions has been estimated.

In Vivo Evaluation of Micronucleus Frequencies in Buccal Mucosal Cells of Orthodontic Patients with and Without Fluoride Use

2021 ◽  
pp. 030157422110373
Author(s):  
Prasad Chitra ◽  
GS Prashantha ◽  
Arun Rao ◽  
Harshvardhan S Jois
Keyword(s):  
Metal Ion ◽  
Orthodontic Treatment ◽  
Biological Tissues ◽  
Control Group ◽  
Time Interval ◽  
Ion Release ◽  
In Vivo Evaluation ◽  
Metal Ion Release ◽  
Mucosal Cells

Introduction: Fluoride agents to prevent white spot lesions are used often during orthodontic treatment. The beneficial effects of fluoride, when consumed within permissible limits on dental structures, are well known. Their implications on underlying biological tissues, however, are unknown. Mouthwashes and dentifrices with fluorides are associated with metal ion release into the mouth with possible cell genotoxicity. Since these cariostatic agents are frequently used during orthodontic therapy, a deeper understanding of the effects of fluoride on oral tissues was considered necessary. Methodology: Three groups of patients (30 each)—group 1 (untreated controls), group 2 (non-fluoridated), and group 3 (Fluoridated) were analyzed. Patients in groups 2 and 3 were bonded with the same bracket prescription and treated with similar archwire sequences. Buccal mucosal cells at 4 specific time periods (before treatment, 1 week, 30 days, and 6 months) were collected, using a wooden tongue depressor, and assessed for any nuclear abnormalities. Comparisons of changes were made with an untreated control group and also between the non-fluoridated and fluoridated groups. Relevant conclusions were drawn after analysis of the results. Results: Greater number of nuclei were observed at the 30-day time interval in the fluoridated group, which was statistically significant at P < .001. Conclusion: Use of fluoridated oral hygiene products in patients undergoing fixed orthodontic treatment with NiTi archwires could increase the risk of micronuclei formation in buccal mucosal cells.

scholarly journals A Systematic Approach to the Application of Soft Tissue Histopathology in Paleopathology

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Christina Grove ◽  
Oliver Peschel ◽  
Andreas G. Nerlich
Keyword(s):  
Soft Tissue ◽  
Connective Tissue ◽  
Time Interval ◽  
Tissue Preservation ◽  
Fat Tissue ◽  
Short Term ◽  
Internal Organs ◽  
Time Intervals ◽  
Pathological Conditions ◽  
Desert Areas

The application of histology to soft tissue remains offers an important technique to obtain diagnostically important information on various physiological and pathological conditions in paleopathology. In a series of 29 cases with mummified tissue ranging between 16 months and c. 5.200 years of postmortem time interval, we systematically investigated paleohistology and the preservation of various tissues. We established a reproducible histological ranking system for the evaluation of mummified tissue preservation. The application of this scheme to the series showed good tissue preservation of tissues with high connective tissue content but also fat tissue and connective tissue rich organs, such as lung tissue, while most other internal organs were less well preserved despite highly different postmortem time intervals. There are some organs with only poor conservation even in short term periods such as the kidneys and CNS. Artificial mummification does not provide better conservation than naturally mummified tissues; “cold” mummies may be much better conserved than those from desert areas. The identification of specific pathologies underlines the potential power of paleohistology.

Relationship of components of the body among mature cows as related to size, lactation potential and possible effects on productivity

1986 ◽  
Vol 43 (2) ◽  
pp. 245-254 ◽  
Author(s):  
T. G. Jenkins ◽  
C. L. Ferrell ◽  
L. V. Cundiff
Keyword(s):  
Milk Production ◽  
Lung Tissue ◽  
The Body ◽  
Performance Potential ◽  
Production Potential ◽  
Internal Organs ◽  
Body Components ◽  
Breed Crosses ◽  
Basal Energy Expenditure ◽  
Relationship Of

ABSTRACTAttributes of lactation and weight of components of the empty body were analysed to evaluate relationships between performance potential of various breeds or breed crosses and the proportion of empty-body components relative to slaughter weight. Differences in major internal organs attributable to breed, breed cross, level of food intake, mature body size and milk production potential were observed. Milk yield was positively related to proportion of lung tissue, tended to be positively related to head and liver tissues but was negatively related to the proportion of warm carcass. As estimated from a three breed diallel, significant additive breed direct effects were observed for several empty-body components. In general, heterosis tended to be non-significant for all proportions of body components with the exception of proportion of head, fore and hind feet and lung tissue. Breed crosses characterized as having greater potential for mature weight were heavier at slaughter and had a greater proportion of structural components such as head, hide and fore and hind feet. Differences among visceral organs associated with production potential for size and milk production are discussed relative to the possible effect on basal energy expenditure and the effect these differences may have on the energy requirements for a cow/calf livestock enterprise.

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