Multi-Omics Comparison of the Spontaneous Diabetes Mellitus and Diet-Induced Prediabetic Macaque Models

The prevalence of diabetes mellitus has been increasing for decades worldwide. To develop safe and potent therapeutics, animal models contribute a lot to the studies of the mechanisms underlying its pathogenesis. Dietary induction using is a well-accepted protocol in generating insulin resistance and diabetes models. In the present study, we reported the multi-omics profiling of the liver and sera from both peripheral blood and hepatic portal vein blood from Macaca fascicularis that spontaneously developed Type-2 diabetes mellitus with a chow diet (sDM). The other two groups of the monkeys fed with chow diet and high-fat high-sugar (HFHS) diet, respectively, were included for comparison. Analyses of various omics datasets revealed the alterations of high consistency. Between the sDM and HFHS monkeys, both the similar and unique alterations in the lipid metabolism have been demonstrated from metabolomic, transcriptomic, and proteomic data repeatedly. The comparison of the proteome and transcriptome confirmed the involvement of fatty acid binding protein 4 (FABP4) in the diet-induced pathogenesis of diabetes in macaques. Furthermore, the commonly changed genes between spontaneous diabetes and HFHS diet-induced prediabetes suggested that the alterations in the intra- and extracellular structural proteins and cell migration in the liver might mediate the HFHS diet induction of diabetes mellitus.

Treatment of Upper Gastrointestinal Bleeding by Percutaneous Transsplenic Varices Embolization in Chronic Hepatic Schistosomiasis Japonicum Patients

To evaluate percutaneous transsplenic varices embolization (PTSVE) in the treatment of upper gastrointestinal bleeding (UGIB) in patients with chronic hepatic schistosomiasis japonicum (CHS), 29 CHS patients (20 males and 9 females) complicated with UGIB were selected as the investigation subjects. The patients were treated by PTSVE under the guidance of X-ray fluoroscopy. The success rate of PTSVE and the rate of complications were observed. In addition, the degrees of varices before and after PTSVE were evaluated by abdominal computed tomography (CT). Results showed that 26 CHS patients (89.6%) were successfully treated with PTSVE. Three cases (10.3%) failed, and two experienced intraperitoneal bleeding within 1 week after PTSVE. The abdominal CT showed a significant decrease of the varices stage in coronary (P < 0.001), esophageal (P = 0.006), and paraesophageal (P = 0.013) varices, but slightly increased perisplenic varices within 1 month of the intervention (P = 0.014). PTSVE may be a safe and effective procedure for the treatment of UGIB in CHS patients, particularly suitable for those with a widened hepatic fissure and exposed hepatic portal vein trunk and an enlarged spleen.

EXTRA-HEPATIC PART OF PORTAL VEIN – A CADAVERIC STUDY IN ADULTS & FOETUSES

Background: Variations of the Hepatic Portal Vein are encountered during abdominal surgeries. Aim: The present study is an observational study of the extrahepatic part of Portal Vein & its variations on cadavers. Materials & Methods: A total of 50 upper abdomen dissections were carried out to delineate extra-hepatic Portal Vein anatomy. The Results: mean length of Portal Vein was 5.96cm. The mean diameter of Portal Vein was 1.35cm. The most common type of formation of Portal Vein was Type I, found in 64%. The Right Gastric Vein commonly emptied into the trunk of the Portal Vein. The Left Gastric Vein commonly emptied into the trunk of the Portal Vein in 87.18%. The most common type of Portal Vein termination was Extra-Hepatic, found in 76% of 50 dissections. The Bifurcation pattern was the most common pattern of Portal Vein termination, found in 94% of dissections. The ndings of the present Conclusion: study highlight the variations that may occur in the hepatic portal venous system in its extra-hepatic part. Sound knowledge of portal venous anatomy is needed for success in the surgeries of the liver & adjacent viscera.