Auto-qPCR; a python-based web app for automated and reproducible analysis of qPCR data

Quantifying changes in DNA and RNA levels is essential in numerous molecular biology protocols. Quantitative real time PCR (qPCR) techniques have evolved to become commonplace, however, data analysis includes many time-consuming and cumbersome steps, which can lead to mistakes and misinterpretation of data. To address these bottlenecks, we have developed an open-source Python software to automate processing of result spreadsheets from qPCR machines, employing calculations usually performed manually. Auto-qPCR is a tool that saves time when computing qPCR data, helping to ensure reproducibility of qPCR experiment analyses. Our web-based app (https://auto-q-pcr.com/) is easy to use and does not require programming knowledge or software installation. Using Auto-qPCR, we provide examples of data treatment, display and statistical analyses for four different data processing modes within one program: (1) DNA quantification to identify genomic deletion or duplication events; (2) assessment of gene expression levels using an absolute model, and relative quantification (3) with or (4) without a reference sample. Our open access Auto-qPCR software saves the time of manual data analysis and provides a more systematic workflow, minimizing the risk of errors. Our program constitutes a new tool that can be incorporated into bioinformatic and molecular biology pipelines in clinical and research labs.

AUTOMATED PREDICTION SYSTEM FOR VEGETATION COVER BASED ON MODIS-NDVI SATELLITE DATA AND NEURAL NETWORKS

Around the world, vegetation cover functioning as shelter to wildlife, clean water, food security as well as treat large part of air pollution problem. Accurate predictive data early warn and provide knowledge for decision makers to reduce the effects of changes in vegetation cover. In this paper, an automated prediction system was developed to forecast vegetation cover. Prediction system based on moderate satellite data spatial resolution and global coverage data. The tools of system automate processing Moderate Resolution Imaging Spectroradiometer (MODIS) images and training neural networks (NN) model based on 60,000 observations to forecast future density of Normalized Difference Vegetation Index (NDVI). Zonguldak data, located in north of Turkey as dense vegetation cover area utilized as case study for system application. This system significantly facilitates predictive process for users than previous long and complex models.