EVALUASI METODE PENGUJIAN ANGKA LEMPENG TOTAL MENGGUNAKAN METODE PETRIFILM AEROBIC COUNT PLATE TERHADAP METODE UJI SNI 01.2332.2006 PADA PRODUK PERIKANAN DI LPPMHP SURABAYA

The study aims to evaluate the testing methods Total Plate Count (ALT) which is easierand faster is Petrifilm Aerobic Plate Count (PAPC) against resistance in TPC testing infishery products, as well as compliance with the Indonesian National Standard (SNI) 012332.3-2006.This study held in Laboratory Quality Control and Testing of FisheryProducts (LPPMHP) Surabaya, East Java. This study three types of cultures breed bacteriaand 24 types of fishery products by using two methods of testing, as the reference test SNI01-2332.3-2006 and in comparison to the PAPC. The results of the test, then analyzedusing statistical calculations to evaluate the suitability of the ISO 4833 test method.Finally, note that ALT testing using PACP in accordance with ISO 01-2332.3-2006 as astandard method of testing, and has a good fit in the ALT test with a sample of fisheryproductsKeywords : total plate count, ISO 4833, petrifilm aerobic count plate, SNI. 01-2332.3-2006

Evaluation of the Petrifilm Aerobic Count Plate for Enumeration of Aerobic Marine Bacteria from Seawater and Caulerpa lentillifera

The enumeration and evaluation of the activity of marine bacteria are important in the food industry. However, detection of marine bacteria in seawater or seafood has not been easy. The Petrifilm aerobic count plate (ACP) is a ready-to-use alternative to the traditional enumeration media used for bacteria associated with food. The purpose of this study was to evaluate the usefulness of a simple detection and enumeration method utilizing the Petrifilm ACP for enumeration of aerobic marine bacteria from seawater and an edible seaweed, Caulerpa lentillifera. The efficiency of enumeration of total aerobic marine bacteria on Petrifilm ACP was compared with that using the spread plate method on marine agar with 80 seawater and 64 C. lentillifera samples. With sterile seawater as the diluent, a close correlation was observed between the method utilizing Petrifilm ACP and that utilizing the conventional marine agar (r = 0.98 for seawater and 0.91 for C. lentillifera). The Petrifilm ACP method was simpler and less time-consuming than the conventional method. These results indicate that Petrifilm ACP is a suitable alternative to conventional marine agar for enumeration of marine microorganisms in seawater and C. lentillifera samples.