Analysis of the genetic diversity of the coastal and island endangered plant species Elaeagnus macrophylla via conserved DNA-derived polymorphism marker

The genetic diversity and genetic structure of five natural populations of the island and coastal endangered plant species Elaeagnus macrophylla were analyzed via conserved DNA-derived polymorphism molecular markers. A total of 289 discernible loci were obtained from 102 individuals via fifteen primers, and 100% of the loci were polymorphic. The observed number of alleles was 1.9654, and the effective number of alleles was 1.2604. Nei’s genetic diversity index was 0.1724 on average, and Shannon’s information index was 0.2869, indicating that Elaeagnus macrophylla had lower levels of genetic diversity than those reported for its continental relatives and other continental species. The average percentage of polymorphic loci was 42.1%, and the maximum and minimum were 80.97% and 14.88%, respectively, which were associated with the Nanji Island and Liugong Island populations, respectively. The populations of Elaeagnus macrophylla were highly differentiated. Cluster analysis revealed that the similarity between the tested samples was related to their geographical location, that the samples from the same island tended to cluster together, and that there was no cross-clustering between samples. The Nanji Island and Da Rushan populations differentiated into two subpopulations. Last, we detected no correlation between genetic distance and geographic distance between populations (Pearson’s correlation coefficient r = 0.256579, p-value = 0.8309).

Analysis of genetic diversity in the coastal and island endangered plant Elaeagnus macrophylla by conserved DNA-derived polymorphism markers

The genetic diversity and genetic structure of five natural populations of the island and coastal endangered plant Elaeagnus macrophylla were analyzed by conserved DNA-derived polymorphism (CDDP) molecular markers. A total of 289 discernible loci were obtained from 102 individuals using fifteen primers, and 100% of the loci were polymorphic. The observed number of alleles (Na) was 1.9654, the effective number of alleles (Ne) was 1.2604, the average of Nei’s genetic diversity index (H) was 0.1724, and Shannon's information index was 0.2869, indicating that E. macrophylla had levels of genetic diversity lower than those reported for continental relatives and other coastal species. The average percentage of polymorphic loci (PPL) was 42.1%, and the maximum and minimum PPL values were 80.97% and 14.88%, belonging to the Nanji Island (NJD) and Liugong Island (LGD) populations, respectively. Populations of E. macrophylla were highly differentiated (Gst=0.3263). The unweighted pair group method with arithmetic mean (UPGMA) clustering results revealed 5 groups: one group comprising the Liugong Island (LGD) and Da Rushan (DRS) populations, one comprising the Lingshan Island (LSD), Laoshan (LS) and Daguan Island (DGD) populations, one comprising the Putuo Island (PTD), and two groups representing the Nanji Island (NJD) samples, which were differentiated into a northwestern group and a southeastern group. There was no cross-clustering among the samples, and the similarity of the relatives was strictly related to geographical location. R software analysis showed no correlation between genetic distance and geographic distance between populations (r = 0.256579, p = 0.8309).

Analysis of genetic diversity in the coastal and island endangered plant Elaeagnus macrophylla by conserved DNA-derived polymorphism markers

The genetic diversity and genetic structure of five natural populations of the island and coastal endangered plant Elaeagnus macrophylla were analyzed by conserved DNA-derived polymorphism (CDDP) molecular markers. A total of 289 discernible loci were obtained from 102 individuals using fifteen primers, and 100% of the loci were polymorphic. The observed number of alleles (Na) was 1.9654, the effective number of alleles (Ne) was 1.2604, the average of Nei’s genetic diversity index (H) was 0.1724, and Shannon's information index was 0.2869, indicating that E. macrophylla had levels of genetic diversity lower than those reported for continental relatives and other coastal species. The average percentage of polymorphic loci (PPL) was 42.1%, and the maximum and minimum PPL values were 80.97% and 14.88%, belonging to the Nanji Island (NJD) and Liugong Island (LGD) populations, respectively. Populations of E. macrophylla were highly differentiated (Gst=0.3263). The unweighted pair group method with arithmetic mean (UPGMA) clustering results revealed 5 groups: one group comprising the Liugong Island (LGD) and Da Rushan (DRS) populations, one comprising the Lingshan Island (LSD), Laoshan (LS) and Daguan Island (DGD) populations, one comprising the Putuo Island (PTD), and two groups representing the Nanji Island (NJD) samples, which were differentiated into a northwestern group and a southeastern group. There was no cross-clustering among the samples, and the similarity of the relatives was strictly related to geographical location. R software analysis showed no correlation between genetic distance and geographic distance between populations (r = 0.256579, p = 0.8309).