Nomenclatural standards and genetic passports of potato cultivars bred by the Tatar Research Institute of Agriculture «Kazan Scientific Center of the Russian Academy of Sciences»

Breeding of potato cultivars adapted to the climatic conditions of the Middle Volga region and resistant to various harmful organisms has always been an urgent task for breeders of the Tatarstan Republic. In the present paper, the potato cultivars bred at the Tatar Research Institute of Agriculture – Kazan Scientific Center of the Russian Academy of Sciences, were taken as an example for demonstrating the results of application and elaboration of methodological approaches that are currently developed at the N.I. Vavilov Institute of Plant Genetic Resources (VIR) for the preparing of nomenclatural standards and their genotyping. At the initiative of the VIR researchers in collaboration with the authors of potato cultivars E.A. Gimaeva and Z. Stashevski plant material was collected and nomenclatural standards for potato cultivars bred at the Kazan Scientific Center of the Russian Academy of Sciences were prepared. Four nomenclatural standards for Tatarstan potato cultivars (‘Zumba’, ‘Kortni’, ‘Reggi’, ‘Samba’) were prepared in accordance with the provisions of the International Code of Nomenclature For Cultivated Plants, registered in the VIR Herbarium Database, and transferred to the VIR herbarium type collection. Besides, voucher specimens of cv. ‘Tango’ and breeding clone ‘Sal’sa’, which is undergoing the State Variety Test at present, were produced. Genetic passports of Tatarstan varieties (‘Zumba’, ‘Kortni’, ‘Reggi’, ‘Samba’) and one breeding clone (‘Sal’sa’)* were developed using DNA samples from plant material transferred by the authors of the cultivars to the VIR herbarium. The genetic passports include information of the polymorphism of eight chromosome-specific microsatellites, data on the presence/ absence of the diagnostic fragments of 15 markers of the 11 R-genes conferring resistance to various harmful organisms and the information about cytoplasm types. A comparison of the data from genetic passports developed using DNA samples from nomenclatural standard specimens with the results of genotyping of 22 samples of Tatarstan cultivars obtained from different sources (e.g., samples undergoing ecogeographic tests within the framework of the Comprehensive Research Plan of the subprogram “Development of potato breeding and seed production in the Russian Federation”; as well as accessions from the VIR in vitro collection) made it possible to assess the authenticity and uniformity of the studied plant material.

Influence of plasmogenes on the productivity of morphogenesis in strawberry (Fragaria x ananassa Duch.)

Plasmogenes are largely located in mitochondria or plastids and they can influence the inheritance of many plant characteristics. This phenomenon is called cytoplasmic inheritance and can be detected on the basis of the expression of a trait in progeny F₁ obtained from single and reciprocal crosses. The aim of this study was to examine the cytoplasmic inheritance of <i>in vitro</i> productivity of morphogenesis in three genotypes of Fragaria x ananassa Duch., i.e. the cultivars 'Dukat', 'Teresa' and the breeding clone no. 590. Single and reciprocal crosses were done according to Griffi ng's method 3. The value of general combining ability (GCA) indicated cv. 'Teresa' as the best maternal component for crossing and 'Dukat' as the worst. The negative reciprocal cross effects (r_ij) revealed the cytoplasmic inheritance for cv. 'Dukat' as maternal form and positive r_ij for the breeding clone no. 590 indicated the nuclear inheritance of morphogenetic ability. Cv. 'Teresa', as maternal component, showed nuclear inheritance of that trait in crossing with cv. 'Dukat' and with 590 cytoplasmic inheritance. The productivity of morphogenesis in strawberry depended on the parental combination and the direction of crossing.

Quantitative assessment of the morphogenetic efficiency in strawberry Fragaria × ananassa Duch

A quantitative assessment of the morphogenetic capability of strawberry was performed. In the experiment, three genotypes of strawberry Fragaria × ananassa Duch. were tested, including two cultivars: ‘Plena’ and ‘Kent’, and one breeding clone no. „394‟. Morphogenetic efficiency was expressed by the mean number of microplants produced by the explant of progeny combinations F1 in two subsequent subcultures. Progeny F1 was obtained after crossings, which were carried out according to Griffing's method 3, with the use of the parental genotypes given above. Quantitative parameters: combining ability and the effect of reciprocal crosses estimated in the progeny F1 allowed to assess the genetic control of the analysed feature. The high positive effect of reciprocal crosses showed that in „Plena‟ the morphogenetic capability is genetically controlled mainly by nuclear genes. On the other hand, aspects of extranuclear inheritance of the analysed trait were observed in ‘Kent’, which proved a weak explant ability of the progeny combination F1 to produce microplants. This resulted in the high negative effect of reciprocal crosses for that cultivar