Effector-driven marker development and cloning of resistance genes against Phytophthora infestans in potato breeding clone SW93-1015

2015 ◽  
Vol 129 (1) ◽  
pp. 105-115 ◽  
Author(s):  
Marit Lenman ◽  
Ashfaq Ali ◽  
Per Mühlenbock ◽  
Ulrika Carlson-Nilsson ◽  
Erland Liljeroth ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Resistance Genes ◽  
Potato Breeding ◽  
Marker Development ◽  
Breeding Clone

scholarly journals Wide Phenotypic Diversity for Resistance to Phytophthora infestans Found in Potato Landraces from Peru

Plant Disease ◽  
2014 ◽  
Vol 98 (11) ◽  
pp. 1530-1533 ◽  
Author(s):  
Willmer Pérez ◽  
Miriam Ñahui ◽  
David Ellis ◽  
Gregory A. Forbes
Keyword(s):  
Phytophthora Infestans ◽  
Resistance Genes ◽  
Potato Breeding ◽  
Phenotypic Diversity ◽  
Small Sample ◽  
Good Effect ◽  
Oomycete Pathogen ◽  
Susceptible Control ◽  
Cultivated Species ◽  
Better Than

The wild and cultivated species of potato have been utilized in potato breeding to good effect but only a very small sample of the available biodiversity has been exploited. In total, 468 accessions of wild and cultivated species of potato were assessed for resistance to the oomycete pathogen Phytophthora infestans using greenhouse assays. Wide phenotypic variation for resistance was found within a species (i.e., among accessions) but not among species which, on average, were similar. Nineteen accessions had resistance levels better than or similar to the variety Chucmarina, which is routinely used by the International Potato Center as a resistant control. Surprisingly, a number of accessions were significantly more susceptible than the susceptible control, Tomasa Condemayta. Frequency histograms of species indicated continuous variation for resistance with little evidence for functional resistance genes.

scholarly journals Characterisation of resistance genes resources against late blight available for Czech potato breeding by means of selected DNA markers

2011 ◽  
Vol 51 (No. 2) ◽  
pp. 82-86 ◽  
Author(s):  
P. Sedlák ◽  
P. Vejl ◽  
M. Melounová ◽  
P. Křenek ◽  
J. Domkářová ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Dna Markers ◽  
Marker Assisted Selection ◽  
Potato Breeding ◽  
Potato Research ◽  
Plant Production ◽  
Potato Chromosome ◽  
Research Institutes ◽  
New Varieties ◽  
Developing Area

Marker assisted selection (MAS) in potato breeding is the most developing area at present time. Methods of DNA markers are developed in all top world potato research institutes and universities oriented on plant production. This paper presents results obtained from the testing of gene resources encompassing different resistance genes against Phytophthora infestans potentially exploitable for Czech potato breeding. Three different DNA markers were studied that are linked to R1 locus in the potato chromosome V operating as a resistance against some races of P. infestans. Markering capability and frequencies of these markers was evaluated with respect to their usage in practical Czech plant breeding that has vital importance in finding the tools as a prerequisite for creating new varieties efficiently.

scholarly journals Mating type, mefenoxam sensitivity, and pathotype diversity in Phytophthora infestans isolates from tomato in Brazil

2010 ◽  
Vol 45 (7) ◽  
pp. 671-679 ◽  
Author(s):  
Bruno Eduardo Cardozo de Miranda ◽  
Nelson Dias Suassuna ◽  
Ailton Reis
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Resistance Genes ◽  
Virulence Genes ◽  
Average Frequency ◽  
Breeding Programs ◽  
Low Frequencies ◽  
Distrito Federal ◽  
Tomato Breeding ◽  
Major Resistance Genes

The objective of this work was to characterize 79 Phytophthora infestans isolates collected in tomato (Solanum lycopersicum) fields, as to mating type, mefenoxam sensitivity, and pathotype composition. The isolates were sampled in 2006 and 2007 in seven Brazilian states as well as in the Distrito Federal. They were characterised as to mating type (n=79), sensitivity to fungicide mefenoxam (n=79), and virulence to three major resistance genes Ph-1, Ph-2, and Ph-3/Ph-4 (n=62). All isolates were of the mating type A1. Resistant isolates were detected in all sampled states, and its average frequency was superior to 50%. No difference was detected in pathotype diversity, neither between subpopulations collected in 2006 and 2007 nor between isolates grouped as resistant or intermediately sensitive to mefenoxam. All major resistance genes were overcome at different frequencies: Ph-1, 88.7%; Ph-2, 64.5%; and Ph-3/Ph-4, 25.8%. Isolates with virulence genes able to overcome all major resistance genes were detected at low frequencies. Tomato breeding programs in Brazil must avoid the development of cultivars with resistance based exclusively on major genes.

scholarly journals The effect of pyramiding Phytophthora infestans resistance genes R Pi-mcd1 and R Pi-ber in potato

2010 ◽  
Vol 121 (1) ◽  
pp. 117-125 ◽  
Author(s):  
M. Y. Adillah Tan ◽  
Ronald C. B. Hutten ◽  
Richard G. F. Visser ◽  
Herman J. van Eck
Keyword(s):  
Phytophthora Infestans ◽  
Resistance Genes

scholarly journals A novel approach to locate Phytophthora infestans resistance genes on the potato genetic map

2009 ◽  
Vol 120 (4) ◽  
pp. 785-796 ◽  
Author(s):  
Mirjam M. J. Jacobs ◽  
Ben Vosman ◽  
Vivianne G. A. A. Vleeshouwers ◽  
Richard G. F. Visser ◽  
Betty Henken ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Genetic Map ◽  
Resistance Genes ◽  
Novel Approach

scholarly journals Marker-assisted pyramiding of potato late blight resistance genes Rpi-rzc1 and Rpi-phu1 on di- and tetraploid levels

2020 ◽  
Vol 40 (9) ◽  
Author(s):  
Emil Stefańczyk ◽  
Jarosław Plich ◽  
Marta Janiszewska ◽  
Paulina Smyda-Dajmund ◽  
Sylwester Sobkowiak ◽  
...  
Keyword(s):  
Late Blight ◽  
Resistance Genes ◽  
Potato Breeding ◽  
Late Blight Resistance ◽  
Breeding Strategy ◽  
Blight Resistance ◽  
Pcr Markers ◽  
Tetraploid Potato ◽  
Potato Wild Relatives ◽  
Tetraploid Level

Abstract Late blight is a disease with the biggest economic impact on potato cultivation worldwide. Pyramiding of the resistance genes originating from potato wild relatives is a breeding strategy that has a potential to produce potato cultivars durably resistant to late blight. Growing such cultivars would allow limiting the intensive chemical control of the disease. The goal of this work was to transfer the late blight resistance gene Rpi-rzc1 from Solanum ruiz-ceballosii to the tetraploid level of cultivated potato and to pyramid it with the Rpi-phu1 gene. We obtained two diploid and, through 4x-2x cross, a tetraploid potato population segregating for the Rpi-rzc1 presence, as well as one diploid and one tetraploid population where both genes were introgressed. In total, 754 progeny clones were tested for resistance to late blight in detached leaflet assays. Pathogen isolates avirulent on plants with both genes and virulent on plants with the Rpi-phu1 were used. The selection was assisted by two PCR markers flanking the Rpi-rzc1 gene and a newly designed, highly specific intragenic marker indicating the Rpi-phu1 gene presence. We obtained 26 diploid and 49 tetraploid potato clones with pyramid of both genes that should enhance the durability and spectrum of their late blight resistance and that can be exploited in potato breeding. The specificity of the marker for the Rpi-phu1 gene and the precision of the Rpi-rzc1 mapping were improved in this work.

scholarly journals Field Evaluation of Potato Resistance Against Phytophthora Infestans under Natural Infection Pressure

2017 ◽  
Vol 76 (1) ◽  
pp. 25-28
Author(s):  
Jarosław Plich ◽  
Beata Tatarowska
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Potato Breeding ◽  
Natural Infection ◽  
Field Evaluation ◽  
Field Resistance ◽  
Field Conditions ◽  
Breeding Programs ◽  
Infection Pressure ◽  
Potato Resistance

Abstract Although development of potato cultivars resistant to late blight has long been one of the primary goals of potato breeding programs, several different methods of field resistance evaluation have been used by potato breeders. Recently, within the EucaBlight network (www.euroblight.net), common methods of late blight evaluation have been agreed on, and these methods are currently highly recommended for use by all those interested in evaluating the resistance of the potato to late blight. Here, we describe a method of evaluating potato resistance in field conditions routinely used at Młochów Research Center, which is compliant with recommendations of the EucaBlight protocol.

scholarly journals Comparison of defense responses of transgenic potato lines expressing three different Rpi genes to specific Phytophthora infestans races based on transcriptome profiling

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9096
Author(s):  
Xiaohui Yang ◽  
Xiao Guo ◽  
Guangxia Chen ◽  
Daofeng Dong ◽  
Fang Liu ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Resistance Genes ◽  
Plant Pathogen ◽  
Biological Process ◽  
Durable Resistance ◽  
Transcriptome Profiling ◽  
Defense Responses ◽  
Wild Type ◽  
Transgenic Lines

Potato late blight, one of the most devastating diseases in potato, is caused by the oomycete Phytophthora infestans. Over 20 resistance genes have been cloned including R1, R3a, and R3b. The distinctions between defense response mechanisms mediated by different resistance genes are still unclear. Here we performed transcriptome profiling in three transgenic lines, R1, R3a, and R3b, and wild-type Desiree under inoculation with two P. infestans isolates, 89148 (race 0) and CN152 (super race), using RNA-seq. Compared with wild type, specific differentially expressed genes (DEGs) were identified in the three transgenic lines. The highest number of DEGs occurred in transgenic R3b, with 779 DEGs in response to isolate 89148 and 864 DEGs in response to infection by CN152, followed by transgenic R1 lines with 408 DEGs for isolate 89148 and 267 DEGs for CN152. Based on gene ontology, the most common GO terms (15 for 89148 and 20 for CN152) were enriched in transgenic R3a and R3b lines. This indicates that the defense pathways mediated by R3a and R3b are more similar than those mediated by R1. Further separate GO analysis of up- or down-regulated DEGs showed that the down-regulated DEGs mainly functioned in mediating the resistance of potato to P. infestans 89148 by response to stress biological process and to CN152 by oxidation reduction biological process. KEGG pathways of DNA replication, plant-pathogen interaction and pentose and glucuronate interconversions are unique for transgenic R1, R3a, and R3b lines in incompatible interactions. Quantitative real-time PCR experimental validation confirmed the induced expression of DEGs in the late blight resistance signaling pathway. Our results will lay a solid foundation for further understanding the mechanisms of plant-pathogen interactions, and provide a theoretical reference for durable resistance in potato.

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