Effect of Alkaloids Isolated fromPhyllodium pulchellumon Monoamine Levels and Monoamine Oxidase Activity in Rat Brain

Phyllodium pulchellum(P. pulchellum) is a folk medicine with a significant number of bioactivities. The aim of this study was to investigate the effects displayed by alkaloids fractions, isolated from the roots ofP. pulchellum, on neurotransmitters monoamine levels and on monoamine oxidase (MAO) activity. Six alkaloids, which had indolealkylamine orβ-carboline skeleton, were obtained by chromatographic technologies and identified by spectroscopic methods such as NMR and MS. After treatment with alkaloids ofP. pulchellum, the reduction of DA levels (54.55%) and 5-HT levels (35.01%) in rat brain was observed by HPLC-FLD. The effect of alkaloids on the monoamines metabolism was mainly related to MAO inhibition, characterized by IC50values of37.35±6.41and126.53±5.39 μg/mL for MAO-A and MAO-B, respectively. The acute toxicity indicated thatP. pulchellumextract was nontoxic.

The state of biogenic monoamine metabolism and processes of their oxidative deamination in patients with gastrocancerogenesis

Biogenic monoamines metabolism and activity of oxidative deamination and their prognostic significance were investigated in patients with gastrocarcinogenesis. Investigation showed dynamic changes in serum levels of biogenic monoamines and activity of platelet MAO-B. Serum dopamine, epinephrine, norepinephrine, DOPA levels decreased, respectively, for II, III and IV stages of gastric cancer. An increase of serotonin and monoamine oxidase activity was observed. Analysis of obtained data showed inhibition of ergotropic body functions, activation of trophotropic processes and processes of biogenic amines deamination were observed in gastric cancer, which might be one of the major reasons for the accumulation of serum ammonia, aldehydes and peroxides-oxidizing agents and endotoxins.