Transcription Factor ChbZIP1 from Alkaliphilic Microalgae Chlorella sp. BLD Enhancing Alkaline Tolerance in Transgenic Arabidopsis thaliana

Saline-alkali soil has become an important environmental problem for crop productivity. One of the most effective approaches is to cultivate new stress-tolerant plants through genetic engineering. Through RNA-seq analysis and RT-PCR validation, a novel bZIP transcription factor ChbZIP1, which is significantly upregulated at alkali conditions, was obtained from alkaliphilic microalgae Chlorella sp. BLD. Overexpression of ChbZIP1 in Saccharomyces cerevisiae and Arabidopsis increased their alkali resistance, indicating ChbZIP1 may play important roles in alkali stress response. Through subcellular localization and transcriptional activation activity analyses, we found that ChbZIP1 is a nuclear-localized bZIP TF with transactivation activity to bind with the motif of G-box 2 (TGACGT). Functional analysis found that genes such as GPX1, DOX1, CAT2, and EMB, which contained G-box 2 and were associated with oxidative stress, were significantly upregulated in Arabidopsis with ChbZIP1 overexpression. The antioxidant ability was also enhanced in transgenic Arabidopsis. These results indicate that ChbZIP1 might mediate plant adaptation to alkali stress through the active oxygen detoxification pathway. Thus, ChbZIP1 may contribute to genetically improving plants’ tolerance to alkali stress.

The Effect of Chemical Sulfide Oxidation on the Oxygenic Activity of an Alkaliphilic Microalgae Consortium Deployed for Biogas Upgrading

The oxygenic photosynthetic activity (OPA) of an alkaliphilic microalgae consortium was evaluated at different concentrations of dissolved sulfide under room temperature and well-defined conditions of irradiance and pH in a tubular closed photobioreactor. The kinetic assays showed that it was optimal at a sulfide concentration of 3.2 mg/L under an external photosynthetically active radiation of 50 and 120 μE/m2 s together with a pH of 8.5 and 9.2. In contrast, the oxygenic photosynthetic activity was insignificant at 15 μE/m2 s with a pH of 7.3, both in the absence and presence of sulfide. Consecutive pulse additions of dissolved sulfide evidenced that the accumulation rate of dissolved oxygen was decreased by the spontaneous chemical oxidation of sulfide with dissolved oxygen in alkaline culture media, mainly at high sulfide levels. At 3.2 mg/L of sulfide, the oxygenic photosynthetic activity was improved by around 60% compared to the treatment without sulfide at external irradiances of 120 μE/m2 s, 30 °C, and pH of 8.5 and 9.2. Additionally, an even higher OPA enhancement (around 85%) was observed in the same previous conditions but using 16 mg/L of sulfide. Thiosulfate was the major end-product of sulfide by oxic chemical reaction, both in biotic and abiotic assays with yields of 0.80 and 0.68, respectively.

Evaluation of Ankistrodesmus falcatus for Bicarbonate-Based Integrated Carbon Capture System (BICCAPS)

This study evaluates the performance of alkaliphilic microalgae Ankistrodesmus falcatus in the Bicarbonate-based Integrated Carbon Capture and Algae Production System (BICCAPS). The system utilized bicarbonate as carbon source for microalgae production. BICCAPS parameters such as pH, algal biomass productivity and CO2 utilization (inorganic carbon conversion, Ci) were observed at different sodium bicarbonate (NaHCO3) loading concentration and type of culture media. The highest productivity was observed at 10 g/L of NaHCO3 loading in BRSP medium at 3.5539 mg/L/day. This value is 30% lower compared to the control experiment (continuously aerated bioreactor). The Ci values of the different system ranges from 1.17 x 10-4 to 1.51 x 10-4 moles/L/day. Both the pH of the BRSP and NPK media at 10 g/L and 30g/L loading of NaHCO3 increased through time. The result shows that A. falcatus has a potential in BICCAPS utilization.