Identification and Expression Analysis of DUF4228 Domain Containing (DDP) Genes in Potato Under Abiotic and Phytohormone Stress

DUF4228 (Domain of unknown function 4228) proteins are widely distributed in plants and performed a vital role in abiotic stress response. In potato (Solanum tuberosum), the study of the DUF4228 gene family is sparse. To understant the role of DUF4228 in potato a comprehensive genome-wide analysis was carried out in potato genome. Further, the StDUAF4228 genes relative expression was also evaluated in various plant tissues. In the present study identified 31 StDUF4228 genes were clustered into six groups. The promoter analysis of StDUF4228 revealed various cis-acting regulatory components response to abiotic and phytohormones stresses. Under selection pressure, gene duplication also showed various positive and purifying selections in StDUF4228 genes. Expression profiling indicated that StDUF4228 genes were broadly expressed in various tissues and organs in potato. Moreover, the StDUF4228 genes profiles expression was characterized through RNA-sequence analysis data under salt and heat treatments. In potato, StDUF4228-4, StDUF4228-14, StDUF4228-21, and StDUF4228-30 represented a high expression under salt and heat stress.Furthermore, 23 genes under IAA treatment and 18 under ABA treatment, showed that IAA and ABA played a vital role in stresses. The RT-qPCR and expression profilling exhibited high expression in root tissues. Moreover multiple miRNA target StDUF4228 genes in potato.These expression results provide primary reference data and functional analysis data for other commercial crops.