Lampu Sein Helm Sepeda Berbasis Voice Recognition

Bicycles are not equipped with the turn signal. For driving safety, a bicycle helmet with a turn signal is designed with voice rrecognition. It is using the Arduino Nano as a controller to control the ON and OFF of turn signal lights with voice commands. This device uses a Voice Recognition sensor and microphone that placed on a bicycle helmet. When the voice command is mentioned in the microphone, the Voice Recognition sensor will detect the command specified, the sensor will automatically read and send a signal to Arduino, then the turn signal will light up as instructed, the Arduino on the helmet will send an indicator signal via the Bluetooth Module. The device is able to detect sound with a percentage of 80%. The tool can work with a distance of <2 meters with noise <71 db.

Use of fluorescent Ca2+ dyes with green fluorescent protein and its variants: problems and solutions

We have studied the degree to which fluorescent Ca2+ indicator dyes, and green fluorescent protein and its variants, can be used together. We find that the most commonly used fluorescent protein, enhanced green fluorescent protein (EGFP), seriously contaminates fura 2 signals. We suggest two alternative combinations for which there is no detectable contamination of the Ca2+ indicator signal by the fluorescent protein. Blue fluorescent protein can be used with the Ca2+ indicator Fura Red; EGFP can be used with the Ca2+ indicator X-Rhod 1. The use of these combinations will permit the accurate measurement of Ca2+ signals in cells transfected with fluorescent proteins.