INVERSION POLYMORPHISM OF NATURAL POPULATIONS ANOPHELES BEKLEMISHEVI STEGNII ET KABANOVA IN WESTERN SIBERIA

The geographical distribution and inversion polymorphism of malaria mosquitoes Anopheles beklemishevi Stegnii et Kabanova in the West Siberia were investigated. X chromosome homozygous cytotypes were defined by fluorescent in situ hybridization of microdissected DNA-probe, labeling the breakpoints region of X chromosome inversions. For the first time the samples, which are homozygous and hemizygous by inversions X1 и X2 were detected. Cytotypes representation and frequencies have not differences between northern and southern (Altay) population of the malaria mosquitoes.

The Development of Cytogenetic Maps for Malaria Mosquitoes

Anopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a Plasmodium transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference assemblies. High-resolution cytogenetic maps are essential for anchoring genome sequences to chromosomes as well as for studying breakpoints of chromosome rearrangements and chromatin protein localization. Here we describe a detailed pipeline for the development of high-resolution cytogenetic maps using polytene chromosomes of malaria mosquitoes. We apply this workflow to the refinement of the cytogenetic map developed for Anopheles beklemishevi.