Putative regulatory functions of SNPs associated with bronchial asthma, arterial hypertension and their comorbid phenotype

Linkage disequilibrium (LD) of single nucleotide polymorphisms (SNPs) of TLR4/AL160272.2 (rs1927914, rs1928298, rs7038716, rs7026297, rs7025144) was estimated in the Slavs of West Siberia. We further investigated an association of SNPs in TLR4/AL160272.2 (rs1927914, rs7038716, rs7025144), SERPINA1 (rs1980616), ATXN2/BRAP (rs11065987), IL2RB (rs2284033), NT5C2 (rs11191582), CARD8 (rs11669386), ANG/RNASE4 (rs1010461), and ABTB2/ САТ (rs2022318) genes with bronchial asthma (BA), arterial hypertension (AH) and their comorbidity. Then, the disease-associated SNPs were annotated in silico in relation to their potential regulatory functions. Strong LD was detected between rs1928298 and rs1927914, as well as rs7026297 and rs7038716 in the Slavs of West Siberia. It was found that the rs1927914 G allele of the TLR4 gene and the rs1980616 C allele of the SERPINA1 gene are associated with the predisposition to BA. These SNPs can affect binding affinity of transcription factors of the Pou and Klf4 families, as well as the expression levels of the TLR4 and SERPINA1 genes. The rs11065987 allele A of the ATXN2/BRAP genes, the rs11669386 A allele of the CARD8 gene, the rs2284033 allele G of the IL2RB gene, and the rs11191582 allele G of the NT5C2 gene were associated with the risk of AH. These variants can alter binding affinity of the Hoxa9, Irf, RORalpha1 and HMG-IY transcription factors, as well as the expression levels of the ALDH2, CARD8, NT5C2, ARL3, and SFXN2 genes in blood cells/vessels/heart, respectively. The risk of developing a comorbid phenotype of AD and AH is associated with the A allele of rs7038716 and the T allele of rs7025144 of the TLR4/AL160272.2 genes, the A allele of rs1010461 of the ANG gene and the C allele of rs2022318 of the ABTB2/CAT genes. Variants rs7038716 and rs7025144 can change the expression levels of the TLR4 gene in blood cells, while rs1010461 and rs2022318 influence the expression levels of the ANG and RNASE4 genes as well as the CAT and ABTB2 genes in blood cells, lungs/vessels/heart.

Dendroarchaeology Potential in Various Natural Areas in West Siberia

Scientists face with many limitations that affect the success of dendrochronological dating working with archaeological (architectural) sites in Western Siberia. Here we analysed 134 sites and carried out the existing limitations of the method in different natural areas. As a result, a connection between the efficiency of dendrochronological dating and the physical and geographical conditions of sites was found. Created algorithm allowed to assess the potential of dendrochronology for the forest-steppe, taiga and forest-tundra areas of Western Siberia. The scientific results presented in the article can be used to solve the problems of chronology and periodization of archaeological and architectural sites of Western Siberia, as well as to increase the overall efficiency of the application of the dendrochronological method.

Soil genetics in its complex stabilization

The problem of the inter-repair time of automobile roads in Russia can be solved by the reinforcing the subgrade soils with cement. Soil is a multicomponent system affecting the deformation and strength properties of the composition (cement and soil). The cement-soil composition has drawbacks, especially in seasonal freezing regions, which affects its durability.In order to increase the composition efficiency, the Nicoflok polymer-mineral additive is used to strengthen subgrade with cement. However, the influence of the type regional and genetic soil on strength properties of the cement-soil + Nicoflok composition is yet studied.The paper presents the factor analysis of the influence of soil genetics on the composition strength properties. The study of the multicomponent system includes the response surface of the soil genetics on the strength properties of the composition. Additional studies are required to move from the qualitative evaluation of the soil genetics on the composition (cement-soil + Nicoflok) to the quantitative evaluation. These studies must be carried out according to a single scheme, which regards the seasonal freezing of subgrade soils in the northern regions of European Russia and West Siberia.

First record of ranavirus (Ranavirus sp.) in Siberia, Russia

Ranaviruses are a group of double-strand DNA viruses that infect fish, amphibians and reptiles. These viruses are responsible for mass fish and amphibian mortality events worldwide, both in the wild and at the fish and amphibian farms. The number of detected epizootics has grown significantly in recent years. In Eastern Europe and Northern Asia, including Russia, very few ranavirosis monitoring studies have been conducted, in contrast with Western Europe and America. In the present work, we used a qPCR assay to survey for the first time the amphibian populations of West Siberia (Russia) for the presence of ranaviruses. In total, we studied 252 tissue samples from six amphibian species, collected across West Siberia from the south to the Arctic regions. We report a single infected sample: a common toad (Bufo bufo) captured near Tyumen city. The phylogenetic analysis showed that the detected virus strain belongs to the CMTV lineage. This is only the second observation of Ranavirus in Russia.

Female festive costume of the Khanty and Mansi in the late 20th — early 21st century: dynamics and functions

In order to determine dynamics and causes of transformation of everyday dress into a festive costume, specifics and functions of the women’s costume of the Khanty and Mansi have been studied. Towards this, the comparative-typological method was employed to study the costume composition, its local features, and differences with respect to the traditional everyday dresses, and the functions of the costume were determined. The study is based upon the materials of ethnographic expeditions carried out in the 1990s–2010s in the regions occupied by the Ob Ugric population (North-West Siberia and Northern Trans-Urals). It has been ascertained that the festive costume commonly comprised a dress, a breast decoration, and a shawl, and in its local variants it was complemeted by other items. The costume was all-season and had common and local elements. The common elements include multi-completeness (it consists of several items), variability according to weather conditions, use of silk and woolen fabrics and beads. The local specifics are manifetsed in the costume composition, silhouette variability, and techniques of decoration. In the end of the 20th — beginning of the 21st century, traditional clothing of the Khanty and Mansi changed in the appearance due to the use of modern synthetic materials (it changed the colour, sillhuette, means and techniques of decoration) and became merely festive. To the large extent those changes were caused by the industrial development on the territory occupied by the Ob Ugric population in the last quarter of the 20th century, and later by the cultural, social, and economic transformations in Russia. The range of use of the traditional clothing shrank due to the spread of factory-made clothing. The growing interest to the ethnic culture stimulated demand for the national costume. It has become made from import synthetic fabrics, because the home-produced cotton fabrics disappeared from the shops. New fabrics changed the appearance of the clothing and its function, as it became merely festive.

New records of Crambidae (Insecta, Lepidoptera) from Omsk Region

Four species of Crambidae are reported from the territory of Omsk Region of Russia. Titanio ledereri (Staudinger, 1870) is new to Asian part of Russia, Loxostege peltalis (Eversmann, 1842), Catoptria verellus (Zincken, 1817) and Ostrinia peregrinalis (Eversmann, 1852) are new to West Siberia.

Role of Institutional Environment in Shaping Political Careers of Young Political Leaders in the Regions of South-West Siberia

Evaluating prospects of bringing youth into Russian political elites has both an acuteness of a scientific challenge and practical relevance in the light of upcoming federal and regional elections of 2021. Based on the surveys of mass political consciousness of the youth and expert polls held in 2018-2020 in Altai Krai and Novosibirsk Oblast, the article has analyzed the visions of young people and their leaders on the effectiveness of career development through participation in youth political organizations, political parties and running for posts in election campaigns. Having measured these visions against the proportion of young people in the elite of the regional offices of political parties and in the lists of candidates running in regional elections, the authors have come to the conclusion about the low acceptance rate of career lift in regional political process. The object of the research comprises regions with different level of social and economic development, which has proved that lack of resource hinders realization of young leaders potential primarily in the regions with high concentration of economic capital and strong political competition.

The fungal literature-based occurrence database for southern West Siberia (Russia)

The paper presents the initiative on literature-based occurrence data mobilisation of fungi and fungi-related organisms (literature-based occurrences, Darwin Core MaterialCitation) to develop the Fungal literature-based occurrence database for the southern West Siberia (FuSWS). The initiative on mobilisation of literature-based occurrence data started in the northern part of West Siberia in 2016. The present project extends the initiative to the southern regions and includes ten administrative territories (Tyumen Region, Sverdlovsk Region, Chelyabinsk Region, Omsk Region, Kurgan Region, Tomsk Region, Novosibirsk Region, Kemerovo Region, Altai Territory and Republic of Altai). The area occupies the central to southern part of the West Siberian Plain and extends for about 1.5 K km from the west to the east from the eastern slopes of the Ural Mountains to Yenisey River and from north to south—about 1.3 K km. The total area equals about 1.4 million km2. The initiative is actively growing in spatial, collaboration and data accumulation terms. The working group of about 30 mycologists from eight organisations dedicated to the data mobilisation was created as part of the Siberian Mycological Society (informal organisation since 2019). They have compiled the almost complete bibliographic list of mycology-related papers for the southern West Siberia, including over 900 publications for the last two centuries (the earliest dated 1800). All literature sources were digitised and an online library was created to integrate bibliography metadata and digitised papers using Zotero bibliography manager. The analysis of published sources showed that about two-thirds of works contain occurrences of fungi for the scope of mobilisation. At the time of the paper submission, the database had been populated with a total of about 8 K records from 93 sources. The dataset is uploaded to GBIF, where it is available for online search of species occurrences and/or download. The project's page with the introduction, templates, bibliography list, video-presentations and written instructions is available (in Russian) at the web site of the Siberian Mycological Society. The initiative will be continued in the following years to extract the records from all published sources. The paper presents the first project with the aim of literature-based occurrence data mobilisation of fungi and fungi-related organisms in the southern West Siberia. The full bibliography and a digital library of all regional mycological publications created for the first time includes about 900 published works. By the time of paper submission, nearly 8 K occurrence records were extracted from about 90 literature sources and integrated into the FuSWS database published in GBIF.

Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems

Upland forest ecosystems are recognized as net sinks for atmospheric methane (CH4), one of the most impactful greenhouse gases. Biological methane uptake in these ecosystems occurs due to the activity of aerobic methanotrophic bacteria. Russia hosts one-fifth of the global forest area, with the most extensive forest landscapes located in West Siberia. Here, we report seasonal CH4 flux measurements conducted in 2018 in three types of stands in West Siberian middle taiga–Siberian pine, Aspen, and mixed forests. High rates of methane uptake of up to −0.184 mg CH4 m−2 h−1 were measured by a static chamber method, with an estimated total growing season consumption of 4.5 ± 0.5 kg CH4 ha−1. Forest type had little to no effect on methane fluxes within each season. Soil methane oxidation rate ranged from 0 to 8.1 ng CH4 gDW−1 h−1 and was negatively related to water-filled pore space. The microbial soil communities were dominated by the Alpha- and Gammaproteobacteria, Acidobacteriota and Actinobacteriota. The major group of 16S rRNA gene reads from methanotrophs belonged to uncultivated Beijerinckiaceae bacteria. Molecular identification of methanotrophs based on retrieval of the pmoA gene confirmed that Upland Soil Cluster Alpha was the major bacterial group responsible for CH4 oxidation.

The gene Sr38 for bread wheat breeding in Western Siberia

Present-day wheat breeding for immunity exploits extensively closely related species from the family Triticeae as gene donors. The 2NS/2AS translocation has been introduced into the genome of the cultivated cereal Triticum aestivum from the wild relative T. ventricosum. It contains the Lr37, Yr17, and Sr38 genes, which support seedling resistance to the pathogens Puccinia triticina Eriks., P. striiformis West. f. sp. tritici, and P. graminis Pers. f. sp. tritici Eriks. & E. Henn, which cause brown, yellow, and stem rust of wheat, respectively. This translocation is present in the varieties Trident, Madsen, and Rendezvous grown worldwide and in the Russian varieties Morozko, Svarog, Graf, Marquis, and Homer bred in southern regions. However, the Sr38 gene has not yet been introduced into commercial varieties in West Siberia; thus, it remains of practical importance for breeding in areas where populations of P. graminis f. sp. tritici are represented by avirulent clones. The main goal of this work was to analyze the frequency of clones (a)virulent to the Sr38 gene in an extended West Siberian collection of stem rust agent isolates. In 2019–2020, 139 single pustule isolates of P. graminis f. sp. tritici were obtained on seedlings of the standard susceptible cultivar Khakasskaya in an environmentally controlled laboratory (Institute of Cytology and Genetics SB RAS) from samples of urediniospores collected on commercial and experimental bread wheat fields in the Novosibirsk, Omsk, Altai, and Krasnoyarsk regions. By inoculating test wheat genotypes carrying Sr38 (VPM1 and Trident), variations in the purity of (a)virulent clones were detected in geographical samples of P. graminis f. sp. tritici. In general, clones avirulent to Sr38 constitute 60 % of the West Siberian fungus population, whereas not a single virulent isolate was detected in the Krasnoyarsk collection. The Russian breeding material was screened for sources of the stem rust resistance gene by using molecular markers specific to the 2NS/2AS translocation. A collection of hybrid lines and varieties of bread spring wheat adapted to West Siberia (Omsk SAU) was analyzed to identify accessions promising for the region. The presence of the gene was postulated by genotyping with specific primers (VENTRIUP-LN2) and phytopathological tests with avirulent clones of the fungus. Dominant Sr38 alleles were identified in Lutescens 12-18, Lutescens 81-17, Lutescens 66-16, Erythrospermum 79/07, 9-31, and 8-26. On the grounds of the composition of the West Siberian P. graminis f. sp. tritici population, the Sr38 gene can be considered a candidate for pyramiding genotypes promising for the Novosibirsk, Altai, and Krasnoyarsk regions.