Signet Ring Cell Adenoma Thyroid – A Case Report

Signet ring cell adenoma is a rare thyroid neoplasm which usually present as a solitary nodule. Histopathologically it can very well be mistaken for a metastatic signet ring cell lesion, as both of them shows cells with intracytoplasmic vacuole and eccentrically pushed nucleus. The intracytoplasmic vacuole in signet ring cell adenoma stain positive for thyroglobulin which help in confirming the diagnosis. Here we describe a case of signet ring cell adenoma of thyroid in a 46-year-old female with its cytological, histological and immunohistochemical features.

Renal Oncocytoma With Both Lymphovascular Invasion and Prominent Intracytoplasmic Vacuole-Like Spaces: A Case Report and Review of the Literature

Here we report a case of renal oncocytoma in a 68 year-old male. The diagnosis was initially made on a needle biopsy 6 years prior to the partial nephrectomy. The case is unique that in addition to the gross and microscopic features commonly seen in renal oncocytomas, both lymphovascular invasion and prominent intracytoplasmic vacuole-like spaces are also present in this tumor. Although vascular invasion is increasingly recognized as compatible with renal oncocytoma, intracytoplasmic vacuoles are a rare and unusual finding that may lead to diagnostic difficulty. The diagnosis of renal oncocytoma was confirmed after immunohistochemistry was performed to argue against succinate dehydrogenase deficient renal cell carcinoma (RCC) and chromophobe RCC. This case highlights the importance for practicing pathologists to recognize the rare co-occurrence of lymphovascular invasion and large intracytoplasmic vacuole-like spaces in renal oncocytoma. Other differential diagnoses may include emerging renal tumor entities, such as the recently-proposed eosinophilic vacuolated tumor.

214 PREANTRAL FOLLICLE BANKING AND ULTRASTRUCTURAL DEFORMITY INDUCED BY DIFFERENT CRYOPRESERVATION PROTOCOLS

Establishment of cryopreserved follicle banks would provide a valuable source of immature oocytes for subsequent follicular culture and embryo pro- duction. In order to develop an optimal cryopreservation protocol, a comparison of ovarian and follicular cyropreservation procedures was performed. Whole ovaries or isolated follicles of B6CBAF1 mice were either frozen slowly or vitrified, and post-thaw follicle growth and oocyte maturation, parthenogenetic activation and embryo development after IVF, and follicle ultrastructure were subsequently monitored. In ovary cryopreservation, intrafollicular oocytes (n = 67-80) can mature (n = 20-21; 25-31%) and develop into blastocysts (n = 8-12; 12-16%) after parthenogenesis. When comparing optimal methods, slow freezing using 1.5 M of DMSO with a 0.5-mL straw resulted in more cleavage than did vitrification using 7.5% ethylene glycol (EG) + DMSO with an EM grid (P < 0.05) after IVF. In follicle cryopreservation, slow freezing (n = 79) yielded only mature oocytes (n = 19; 24%), but none were parthenogenetically activated. Major cryoinjury after ovary cryopreservation was intracytoplasmic vacuole formation and mitochondrial deformity. In follicular cells, vitrification induced more damage in mitochondria than did slow freezing, whereas both induced mitochondrial damages and vacuole formation in ooplasm. Slow freezing did not damage zona pellucida. In conclusion, banking of the whole ovary yields better outcome of follicle culture than follicle banking and, compared with vitrification, slow freezing efficiently supports post-thaw survival and ultrastructure normality in ovary cryopreservation. However, each protocol induces cell-specific damage. This research was supported by a grant (Code 200504676) from BioGreen 21 Program, Rural Development Administration, Republic of Korea. The authors also acknowledge a world-class university program supported by the Korean Ministry of Education, Science, and Technology.