214 PREANTRAL FOLLICLE BANKING AND ULTRASTRUCTURAL DEFORMITY INDUCED BY DIFFERENT CRYOPRESERVATION PROTOCOLS

2010 ◽  
Vol 22 (1) ◽  
pp. 265
Author(s):  
G. A. Kim ◽  
H. Y. Kim ◽  
J. W. Kim ◽  
G. Lee ◽  
E. S. Lee ◽  
...  

Establishment of cryopreserved follicle banks would provide a valuable source of immature oocytes for subsequent follicular culture and embryo pro- duction. In order to develop an optimal cryopreservation protocol, a comparison of ovarian and follicular cyropreservation procedures was performed. Whole ovaries or isolated follicles of B6CBAF1 mice were either frozen slowly or vitrified, and post-thaw follicle growth and oocyte maturation, parthenogenetic activation and embryo development after IVF, and follicle ultrastructure were subsequently monitored. In ovary cryopreservation, intrafollicular oocytes (n = 67-80) can mature (n = 20-21; 25-31%) and develop into blastocysts (n = 8-12; 12-16%) after parthenogenesis. When comparing optimal methods, slow freezing using 1.5 M of DMSO with a 0.5-mL straw resulted in more cleavage than did vitrification using 7.5% ethylene glycol (EG) + DMSO with an EM grid (P < 0.05) after IVF. In follicle cryopreservation, slow freezing (n = 79) yielded only mature oocytes (n = 19; 24%), but none were parthenogenetically activated. Major cryoinjury after ovary cryopreservation was intracytoplasmic vacuole formation and mitochondrial deformity. In follicular cells, vitrification induced more damage in mitochondria than did slow freezing, whereas both induced mitochondrial damages and vacuole formation in ooplasm. Slow freezing did not damage zona pellucida. In conclusion, banking of the whole ovary yields better outcome of follicle culture than follicle banking and, compared with vitrification, slow freezing efficiently supports post-thaw survival and ultrastructure normality in ovary cryopreservation. However, each protocol induces cell-specific damage. This research was supported by a grant (Code 200504676) from BioGreen 21 Program, Rural Development Administration, Republic of Korea. The authors also acknowledge a world-class university program supported by the Korean Ministry of Education, Science, and Technology.

2021 ◽  
Author(s):  
Ketevan Inasaridze

In 2020, the situation created by the COVID-19 pandemic in the world, including in Georgia, led to the emergence of a number of mental health problems in the population. The Ministry of Education, Science, Culture and Sports of Georgia has started to establish a psychological hotline to solve the psychological problems in the Georgian educational space. This article aims to increase the knowledge of the psychologists involved in the psychological hotline service about the main issues of psychological counseling


2021 ◽  
Author(s):  
Grupa Autora

The International Thematic Proceedia titled „Psychology in the world of science” is a publication from the 16th International Conference “Days of Applied Psychology” held on September 25th & 26th 2020 at the Faculty of Philosophy, University of Niš. This is a traditional annual nonprofit conference which has been organized since 2005 by the Department of Psychology of the Faculty of Philosophy, University of Niš, with the support and co-financing of the Ministry of Education, Science and Technological Development of the Republic of Serbia. The conference started with the idea of gathering researchers and practitioners who discuss the link between science and practice in different psychological areas. From the very start, this gathering has welcomed international participants, and year after year this number is on the rise. This scientific publication contains 18 peer-reviewed articles which can be classified as original scientific papers and as review papers. The authors of these manuscripts come from six countries: Portugal, Bosnia and Herzegovina, Slovenia, Bulgaria, Turkey, and Republic of Serbia.


Stanovnistvo ◽  
2018 ◽  
Vol 56 (2) ◽  
pp. 1-21
Author(s):  
Marko Galjak

The goal was to examine demographic differences between former communist regions and other regions of the EU. Besides providing a regional overview of EU?s demographic differences, we question whether the subnational approach offers any new insights into the East-West divide. This cross-sectional study was conducted on 1,155 EU?s NUTS3 regions from 2014. These regions are grouped in two groups: regions that were part of a com-munist country, and other regions. Mortality, fertility and age structure indicators were tested between the two groups of regions. GDP/c was used to control for differences in economic development by segmenting the regions into tree brackets: low, medium, and high. The differences were then tested for each indicator. Regional variation within countries for each indicator was also assessed. The gaps exist at regional level and are the widest with mortality and fertility schedule, regardless of GDP/c. Former communist regions on average tend to be slightly younger. Analysis of regional variation showed that subnational approach was warranted when studying East-West demographic disparities, especially when it comes to fertility schedule. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 47006: Istrazivanje demografskih fenomena u funkciji javnih politika u Srbiji]


2017 ◽  
Vol 13 (19) ◽  
pp. 146
Author(s):  
John M. Mbunde

This paper discusses the head teachers’ role in facilitating school facilities that influence pupils’ performance in Kenya Certificate of Primary School (KCPE). The objective was to establish the head teachers’ role in facilitating school facilities that influence pupils’ performance in Kenya Certificate of Primary School. The research was based on the Max Weber’s Theory of Bureaucracy. The target population was 612 head teachers, senior teachers and accounts’ clerks in Nairobi County. The sample size was 123. The research employed descriptive survey design. The schools selected were 14 from Westland and Dagoretti districts and 13 from Lang’ata district through simple random sampling. The head teachers and accounts’ clerks were selected by purposive sampling methods. The research instruments used were the interview and document analysis guides. Validation of both instruments was done by the expert judgment review by supervisors from the Department of Educational Administration and Planning of the University of Nairobi. Descriptive statistics was used to analyze the data which was presented in a table. The study established that all the 41 sampled schools had fairly adequate exercise books, buildings and furniture; inadequate textbooks, radios, equipment, computers, displays, charts and playgrounds. The study concluded that the head teachers had not facilitated adequate school facilities that influence pupils’ performance in Kenya Certificate of Primary School (KCPE). It was recommended that the Ministry of Education, Science and Technology should increase funding of schools to enable the head teachers to procure more school facilities to enhance pupils’ performance in Kenya Certificate of Primary School.


2019 ◽  
Vol 18 (3) ◽  
pp. 35-45
Author(s):  
Vilma Čingienė ◽  
Mindaugas Gobikas

This article aims to analyse the process of formation of sports public policy in Lithuania within the theoretical context of hierarchy governance. This study consisted of collection and analysis of official documents regarding sports public policy formation from 2011 until 2018. The data collection was aimed at uncovering of key components of the process of public policy formation – environmental analysis, strategic planning, competence and decision making power, and stakeholders. The main findings of the research concluded that Lithuanian sports governance, along with the majority of other European countries, is defined as bureaucratic configuration. The main responsibility within the process of sports public policy formation falls on the Ministry of Education, Science and Sports and active national non-government sports organisations, while principal objectives of the Lithuanian sports public policy formation are laid out in strategic documents. However, the implementation needs to be centred on institutional and personal responsibility, proper environmental regard and tolerance, and the ability to listen and to reach an agreement.


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