Incorporating 3D virtual environments into psychological experiments offers an innovative solution for balancing experimental control and ecological validity. Their flexible use, however, has been limited to those researchers with extensive coding experience because the field lacks accessible development tools. We created OpenMaze, an open-source toolbox for the Unity game engine, to overcome this barrier. OpenMaze offers researchers the ability to conduct a wide range of 3D spatial navigation experiment paradigms in fully customized 3D environments. Crucially, because all experiment configurations are defined in user-friendly JavaScript Object Notation (JSON) files, our toolbox allows even those with no prior coding experience to build bespoke tasks. OpenMaze is also compatible with a variety of input devices and operating systems, broadening its possible applications. To demonstrate its advantages, we review and contrast other available software options before guiding the reader through building an experiment in OpenMaze.