Arc Discharge–Induced Ignition of Combustibles Placed on a Damaged AC Power Supply Cord

One of the major causes of unintentional electrical fires is short circuit of an electrically and/or mechanically damaged alternating power supply cord. Detecting of such an event and interrupting the power supply may be beyond the capability of a conventional electro-mechanical circuit breaker. A lot of research papers have been published related to arc fault of wiring and its detection method. Furthermore, arc fault circuit interrupters have been put into practical use. The objective of the present paper is to understand fault of damaged power supply cord under two selected situations observed in practical use or considered suitable to understand fire ignition. Using two kinds of samples similar to but different from samples prescribed in UL1699 standard, the ignition mechanism of combustibles is discussed based on the results of laboratory experiments. The findings herein underscore the important role of the arc in the ignition of combustibles that are placed on the damaged part of a power supply cord, which is normally followed by a short circuit of broken element conductors or breakage of intact element conductors. Moreover, a possible arc detection feature in the two situations is discussed based on a distorted voltage waveform.

STABLE NON-MUTATOR STOCKS OF MAIZE HAVE SEQUENCES HOMOLOGOUS TO THE Mu1 TRANSPOSABLE ELEMENT

ABSTRACT Mutator stocks of maize produce mutants at many loci at rates 20- to 50-fold above spontaneous levels. Current evidence suggests that this high mutation rate is mediated by an active transposable element system, Mu. Members of this transposable element family are found in ~10-60 copies in Mutator stocks. We report here an initial characterization of previously undetected sequences homologous to Mu elements in eight non-Mutator inbred lines and varieties of maize that have a normal low mutation rate. All stocks have ~40 copies of sequences homologous only to the terminal repeat and show weak homology to an internal probe. In addition, several of the stocks contain an intact Mu element. One intact Mu element and two terminal-specific clones have been isolated from one non-Mutator line, B37. The cloned sequences have been used to demonstrate that in genomic DNA the intact element, termed Mu1.4B37, is modified, such that restriction sites in its termini are not accessible to cleavage by the HinfI restriction enzyme. This modification is similar to that observed in Mutator lines that have lost activity. We hypothesize that the DNA modification of the Mu-like element may contribute to the lack of Mutator activity in B37.

EVOLUTION OF TRANSPOSONS: NATURAL SELECTION FOR Tn5 IN ESCHERICHIA COLI K12

ABSTRACT A novel in vivo effect of the transposable element Tn5 has been observed in chemostats when certain isogenic Tn5 and non-Tn5 strains of Escherichia coli compete for a limiting carbon source in the absence of kanamycin. The Tn5-bearing strain has a more rapid growth rate and increases in frequency from 50% to 90% within the first 15 to 20 generations. The effect occurs when Tn5 is inserted at a variety of chromosomal locations or when the element is carried by an episome, but it is strain specific, having been observed in two out of three strains examined. (For reasons unknown, the effect has not been observed with derivatives of strain CSH12.) Although the growth-rate advantage of Tn5 is independent of nutrient concentration and generation time, it can be reduced by prior adaptation of the strains to limiting conditions, and the amount of reduction is proportional to the length of prior adaptation. The growth-rate effect is evidently not caused by beneficial mutations induced by Tn5 transposition, as Tn5-bearing strains selected in chemostats retain their initial Tn5 position and copy number. However, the effect does not occur in Tn5-112, a transpositionless deletion mutation missing the transposase-coding region of the right-hand IS sequence flanking the element. Since Tn5-112 retains a functional kanamycin-phosphotransferase gene, this gene is not responsible for the growth-rate effect. Thus, the effect evidently requires transposase function, but it does not involve actual transposition of the intact element. Altogether, these data provide a mechanism for the maintenance of Tn5 in bacterial populations in the absence of kanamycin, and they suggest a model for the proliferation and the maintenance of IS sequences and transposable elements in the absence of other identifiable selection pressures.