In emergency situations, exit sign lightings play a role in reducing evacuation time for occupants by providing accurate and prompt information on the correct evacuation direction. In other words, it can be said that the better the recognition of exit sign lighting, the greater the probability of securing the safety of life because the evacuation route information is given to the occupants and eventually the time taken for route selection and movement is shortened. In this study, human reaction time was measured for 27 kinds of graphic symbol color combinations of exit sign lightings in the visible light region, and through this, a graphic symbol color combination that can minimize reaction time to exit sign lightings was derived. As a result, it was found that the color combination of green and white background color, which is currently mainly used in Korea, has the shortest reaction time.