Comparative analysis of Qualitative and Quantitative Phytochemical screening, in vitro antioxidant and antibacterial activity of Mangifera indica and Artocarpus heterophyllus

Medicinal plants have known to play a wide role in the development of human culture. Artocarpus heterophyllus and Mangifera indica are the well-known evergreen tropical fruiting tree families abundantly grown in Asia. Different parts of both the trees such as bark, leaves, fruits, and flowers are used in traditional medicine. The present study aims to screen the qualitative and quantitative phytochemical present, in-vitro antioxidant, and anti-microbial activity of Artocarpus heterophyllus and Mangifera indica leaf extracts and combined extract of both the leaves. The qualitative phytochemical results indicated the presence of phytochemicals such as alkaloids, flavonoids, phytosterols, tannins, and saponins. The quantitative phytochemical results revealed that M.indica has high phenolic and flavonoid content. The Combined extract showed significant antioxidant activity when compared to both the extracts. All the extracts showed significant antimicrobial activity.

PHYTOCHEMICAL SCREENING, IN VITRO ANTIOXIDANT AND ANTI PROTEASE ACTIVITIES OF METHANOLIC EXTRACT OF NILGIRIANTHUS HEYNEANUS NEES STEM

Objective: The aim of this study was focussed on phytochemical screening, in vitro antioxidant and antiprotease activities of methanolic extract of Nilgirianthus heyneanus stem. Methods: The stem of the plant was washed thoroughly, shade dried and coarsely powdered. The powdered material of Niligirianthus heyneanus stem was extracted with methanol using soxhlet apparatus. Preliminary phytochemical screening for carbohydrates, proteins, alkaloids, phytosteroids, flavonoids, glycosides, polyphenolics, saponins, tannins was done by following standard procedure. In vitro antioxidant activities of methanolic extract were assessed using DPPH, ABTS and total antioxidant capacity. In vitro anti-protease activity of the plant was analysed using trypsin as an enzyme and BAEE (N-benzoyl-L-arginine ethyl ester) as a substrate. Results: The results showed that phytochemicals such as carbohydrates, proteins and amino acids, flavonoids, glycosides, tannins and polyphenolics are present in the methanolic extract of Niligirianthus heyneanus stem. The in vitro antioxidant and antiprotease activities of Niligirianthus heyneanus stem clearly showed that the plant has antioxidant and antiprotease activity. Conclusion: From this work, it can be concluded that Niligirianthus heyneanus stem has the potential to be a strong antioxidant and protease inhibitor.

Phytochemical screening, invitro antioxidant and anti-inflammatory activity of polyphenolic fraction extracted from Camellia sinensis bud extract

Tea is one of the most widely consumed beverages in the world today, second only to water, well ahead of coffee, beer, wine and carbonated soft drinks. The tea plant, Camellia sinensis, is a member of the Theaceae family and black, oolong, white and green tea are produced from its leaf and buds. Tea beverage is an infusion of the dried leaves of Camellia sinensis. The present study aims to screen the phytochemicals screening, in vitro antioxidant and anti-inflammatory activity of polyphenolic compound extracted from Camellia sinensis bud extract. The qualitative phytochemical results revealed the presence of important phytochemicals like alkaloids, flavonoids, phenols, and tannins. The C. sinensis buds could be a rich source of antioxidants. The extract showed significant antioxidant and anti-inflammatory activity. Total phenolic content of the bud’s extract was found to be 123 mg/GAE. The extract possesses a significant antioxidant and anti-inflammatory activity.