Variation of Ethnomycological Knowledge in a Community from Central Mexico

We analyze the effects of the ethnomycological knowledge depending on the age range of its users and how far their homes are from the forest areas. As a study model, the traditional mycological knowledge of San Pedro Tlalcuapan SPT, in Central Mexico was used. During 2017, 135 semi-structured interviews were conducted with people of three age ranges and living in three areas of the community located at different distances from the forest. Data was analyzed using descriptive statistics and analyses of non-metric multidimensional scaling, which were then applied to calculate two indices. The effect of age and origin of the individuals on bio-cultural relevance was assessed using multiple linear regression models.Fifty species of edible wild mushrooms were registered, those mentioned most often were of the Russuladelica complex., Amanita basii and Boletus aff. edulis. Ninety-five percent of the people interviewed consumed wild mushrooms on average two times a week, while 57% collected them. Wild mushrooms are mainly recognized for their food and ecological importance. Ordination and regression analyses show that people closest to the forest, as well as older people, are able to identify more of the wild mushrooms and have greater bio-cultural mushroom awareness. Results show that there is a risk that traditional knowledge about wild mushrooms will disappear, since it is only popular in a part of the community and only at some age ranges, indicating that there is a disconnect in the transfer of knowledge.

Antimycotic Activity of Leaf Extracts of Medicinal Plants Against Dermatophytes

Plant products have been used as medicines against fungal infectious diseases. In this research antimycotic activity of the leaf extracts of five medicinal plants (Nerium indicum, Catheranthus roseus, Lantana camera, Ziziphus mauritiana) were tested against three dermatophytes (Trichophyton mentagrophytes, Trichophyton rubrum and Microsporum. gypseum). Development of more effective and less toxic antimycotic agents is required for the treatment of dermatophytosis. The plant materials were extracted with methanol, ethanol and diethyl ether solvent to investigate their antimycotic activities in Vitro. Ethanol and methanol extracts of all selected medicinal plants were showed the positive activity against all tested dermatophytes. Diethyl ether extract was showed lowest activity against T. mentagrophytes and T. rubrum and showed moderate activity against M. gypseum. The three dermatophytes differed with regard to their susceptibility to plant extracts.

Influence of Carbon - Nitrogen Supplements and pH on Growth of Sugarcane Stem rot Pathogen Fusarium Solani NVS671

Fusarium solani NVS671 identified from infected sugarcane stem of Co 671 as a new pathogen was subjected to various cultural conditions to understand its physiological profile. In continuation with our previous work, cultural analysis was carried out under in vitro condition by supplementing various carbon and nitrogen sources in Czapek-Dox agar (CDA). Under different hydrogen ion concentrations, it was found that the growth of Fusarium solaniNVS671 was less at pH 4 and pH 10 and could reach up to 5 cm after 7 days of incubation on Potato Dextrose Agar (PDA). It is observed that the pH around 7 to 8 was optimum for the growth of F. solani. Ten different nitrogenous (N) sources and nine different carbon sources were tested on CDA medium to know their effect on the mycelial growth rate and characteristics of the fungus. Among the N sources evaluated, ammonium chloride (7.96±0.11 cm) was found to be most efficient for mycelial growth promotion followed by ammonium nitrate (7.7±0.1 cm) and ammonium sulphate (7.3±0.1 cm). The most preferred carbon source recorded to promote best radial mycelial growth was starch (7.96±0.05 cm) and sucrose (7.93±0.05 cm). Capabilities of using different carbon and nitrogen sources and ability to grow at different pH levels may allow species to adapt to specific soil conditions.This study is important to understand the physiology and metabolite preference of F. solani.

Saccharomyces Kluyveri Fungemia in a Patient with Human Immunodeficiency Virus Infection

We report a case of a young woman with acquired immune deficiency syndrome admitted with fever and abdominal pain. Saccharomyces kluyveri was isolated in blood culture. She was successfully treated with fluconazole. This case demonstrates the pathogenicity of Saccharomyces Kluyveri in immunocompromised host.