Cutaneous T-cell lymphoma. Evaluation of pretreatment skin biopsy specimens by a panel of pathologists

1992 ◽  
Vol 128 (4) ◽  
pp. 501-507 ◽  
Author(s):  
J. E. Olerud
Keyword(s):  
T Cell ◽  
Skin Biopsy ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Cutaneous T Cell Lymphoma ◽  
Biopsy Specimens

Small cell variant of CD30+ primary cutaneous T-cell lymphoma with epidermotropism that completely regressed after incisional skin biopsy

2006 ◽  
Vol 155 (2) ◽  
pp. 484-487 ◽  
Author(s):  
T. Kamiya ◽  
K. Saga ◽  
K. Yanagisawa ◽  
R. Kaneko ◽  
T. Yamashita ◽  
...  
Keyword(s):  
T Cell ◽  
Skin Biopsy ◽  
Cell Lymphoma ◽  
Small Cell ◽  
T Cell Lymphoma ◽  
Cutaneous T Cell Lymphoma ◽  
Small Cell Variant ◽  
Cell Variant

scholarly journals Determining the immune environment of cutaneous T-cell lymphoma lesions through the assessment of lesional blood drops

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kan Torii ◽  
Yukinori Okada ◽  
Akimichi Morita
Keyword(s):  
T Cells ◽  
T Cell ◽  
Skin Biopsy ◽  
Mycosis Fungoides ◽  
Assessment Tool ◽  
Cell Lymphoma ◽  
Inflammatory Diseases ◽  
T Cell Lymphoma ◽  
Cell Populations ◽  
Cutaneous T Cell Lymphoma

AbstractDetailed analysis of the cells that infiltrate lesional skin cannot be performed in skin biopsy specimens using immunohistochemistry or cell separation techniques because enzyme treatments applied during the isolation step can destroy small amounts of protein and minor cell populations in the biopsy specimen. Here, we describe a method for isolating T cells from drops of whole blood obtained from lesions during skin biopsy in patients with cutaneous T-cell lymphoma. Lesional blood is assumed to contain lesional resident cells, cells from capillary vessels, and blood overflowing from capillary vessels into the lesion area. The lesional blood showed substantial increases in distinct cell populations, chemokines, and the expression of various genes. The proportion of CD8+CD45RO+ T cells in the lesional blood negatively correlated with the modified severity-weighted assessment tool scores. CD4+CD45RO+ T cells in the lesional blood expressed genes associated with the development of cancer and progression of cutaneous T-cell lymphoma. In addition, CD8+CD45RO+ T cells in lesional blood had unique T-cell receptor repertoires in lesions of each stage. Assessment of lesional blood drops might provide new insight into the pathogenesis of mycosis fungoides and facilitate evaluation of the treatment efficacy for mycosis fungoides as well as other skin inflammatory diseases.

scholarly journals ICOS is widely expressed in cutaneous T-cell lymphoma, and its targeting promotes potent killing of malignant cells

2020 ◽  
Vol 4 (20) ◽  
pp. 5203-5214
Author(s):  
Florent Amatore ◽  
Nicolas Ortonne ◽  
Marc Lopez ◽  
Florence Orlanducci ◽  
Rémy Castellano ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lymphoma ◽  
Xenograft Model ◽  
Treg Cells ◽  
T Cell Lymphoma ◽  
Therapeutic Trial ◽  
Cutaneous T Cell Lymphoma ◽  
Malignant Cells ◽  
Biopsy Specimens ◽  
Mouse Xenograft Model

Abstract The treatment of advanced-stage cutaneous T-cell lymphoma (CTCL) remains an unmet medical need. Mogamulizumab, anti-KIR3DL2, and brentuximab vedotin (BV), an anti-CD30 antibody–drug conjugate (ADC) coupled with monomethyl-auristatin-E (MMAE), provided encouraging results, but new targeted therapies are needed. Inducible T-cell costimulator (ICOS), a T-cell costimulatory receptor, is a promising therapeutic target, not only because it is expressed by malignant T cells in CTCL but also because of its connection with the suppressive activity of regulatory T (Treg) cells. Immunohistochemical analysis revealed that ICOS was widely expressed by malignant cells in skin biopsy specimens from 52 patients with mycosis fungoides and Sézary syndrome (SS), as well as in involved node biopsy specimens from patients with SS. Furthermore, flow cytometry demonstrated its strong expression by circulating tumor cells in all our patients with SS. Percentages of ICOS+ Treg cells were significantly higher in patients with SS than in healthy donors. We then investigated the preclinical efficacy of anti-ICOS ADCs generated by coupling murine anti-ICOS monoclonal antibodies with MMAE and pyrrolobenzodiazepine. In 3 CTCL cell lines (Myla, MJ, and HUT78), we observed a significant dose-dependent decrease in cell viability in the presence of anti-ICOS ADCs. In addition, anti-ICOS-MMAE ADCs had an in vitro and in vivo efficacy superior to BV in a mouse xenograft model (MyLa). Finally, we assessed the efficacy of anti-ICOS ADCs in ICOS+ patient-derived xenografts from patients with SS and angioimmunoblastic T-cell lymphoma. Collectively, our findings provide the preliminary basis for a therapeutic trial.

scholarly journals Skin liquid biopsy method for assessing the immune environment of cutaneous T-cell lymphoma lesions

2021 ◽  
Author(s):  
Kan Torii ◽  
Yukinori Okada ◽  
Akimichi Morita
Keyword(s):  
T Cells ◽  
T Cell ◽  
Skin Biopsy ◽  
Liquid Biopsy ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Enzyme Treatment ◽  
Cell Populations ◽  
Cutaneous T Cell Lymphoma ◽  
Biopsy Method

Abstract (179/200 words) Detailed analysis of cells infiltrating lesional skin cannot be performed in skin biopsy specimens by immunohistochemistry or cell separation techniques because small amounts of protein and minor cell populations in the biopsy specimen might be destroyed by enzyme treatment in the isolation step. Here, we describe a skin liquid biopsy method that enables T cell isolation in small amounts of lesional whole blood from patients with cutaneous T-cell lymphoma. Lesional blood, assumed to contain lesional resident cells, cells from capillary vessels, and blood overflowing from capillary vessels in the lesion area, was obtained during regular skin biopsy. The lesional blood showed substantial increases in distinct cell populations, chemokines, and expression of various genes. CD8 + CD45RO + T cells in the lesional blood negatively correlated with the modified severity-weighted assessment tool scores. CD4 + CD45RO + T cells in the lesional blood expressed genes associated with the development of cancer and progression of cutaneous T-cell lymphoma. The skin liquid biopsy technique might provide new insight into the pathogenesis of mycosis fungoides and facilitate evaluation of the treatment efficacy for other skin inflammatory diseases.

Cutaneous T-Cell Lymphoma Incidence Rising

2008 ◽  
Vol 39 (6) ◽  
pp. 15
Author(s):  
BRUCE JANCIN
Keyword(s):  
T Cell ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Cutaneous T Cell Lymphoma

Cutaneous T cell lymphoma associated with recurrent panniculitis and reactive histiocytosis.

1987 ◽  
Vol 49 (3) ◽  
pp. 447-453 ◽  
Author(s):  
Sadanori NAGAO ◽  
Nobuo ITO ◽  
Hiroshi TAKAHASHI ◽  
Kyoko TASAKI ◽  
Yoshihiko TAKIGUCHI ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Cutaneous T Cell Lymphoma
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