In Indonesia, the incidence of medical error is quite high as evidenced by the existence of hospital incident reports, where in 2010 there were 75% of unexpected events and in 2011 as many as 60% of cases of surgical cases or patients with surgery. Medical error is one of the unwanted events, which occurred in various countries. Therefore it begins to develop a patient safety system. In order to carry out these functions, hospital should be able to carry out management based on customer oriented and patient safety by implementing a safety attitude culture. The purpose of this study was to determine the safety attitude culture evaluation of nurses in surgery rooms of PKU Muhammadiyah Gamping. This research was a research using a mixed methods research approach; namely a quantitative method with a descriptive approach and qualitative methods with a case study approach. The population in this study was nurses in surgery room with a total sampling technique of 20 people. The questionnaire in this study referred to the Surgery room Version of Safety Attitudes Questionnaire. Quantitative data analysis used descriptive analysis; while qualitative analysis was performed by data reduction, data presentation, and drawing conclusions/verification. The results shows that overall evaluation of safety culture attitude of nurses in the surgery room summed up in the high category (75,0 %). Meanwhile based on the safety attitude culture component, namely safety climate is in the high category (85,0%), team working climate is in the high category (90,0%), stress recognition is in the high category (65,0%), management perceptions is in the moderate category ( 75,0%), working conditions is in the high category (60,0%), job satisfaction is in the high category (90,0%).