Determination of Activity of the Enzymes Hypoxanthine Phosphoribosyl Transferase (HPRT) and Adenine Phosphoribosyl Transferase (APRT) in Blood Spots on Filter Paper

2015 ◽  
Vol 86 (1) ◽  
Author(s):  
Kasie Auler ◽  
Robyn Broock ◽  
William L. Nyhan
Keyword(s):  
Filter Paper ◽  
Hypoxanthine Phosphoribosyl Transferase ◽  
Phosphoribosyl Transferase ◽  
Blood Spots ◽  
Adenine Phosphoribosyl Transferase ◽  
Determination Of Activity

Determination of Chloroquine in Dried Blood Spots on Filter Paper

1986 ◽  
Vol 8 (2) ◽  
pp. 211-213 ◽  
Author(s):  
Y. Bergqvist ◽  
Ö. Ericsson ◽  
M. Rais
Keyword(s):  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots

Fluorometric micromethod for determination of arginase activity in dried blood spots on filter paper.

1980 ◽  
Vol 26 (8) ◽  
pp. 1198-1200 ◽  
Author(s):  
A P Orfanos ◽  
E W Naylor ◽  
R Guthrie
Keyword(s):  
Filter Paper ◽  
Colorimetric Method ◽  
Dried Blood Spots ◽  
Arginase Activity ◽  
Kinetic Reaction ◽  
Hematological Disorders ◽  
Blood Spots ◽  
Blood Specimens ◽  
The Stability

Abstract We describe a microfluorometric method for determination of arginase activity in dried blood spots on filter paper. The arginase in discs punched from such dried blood specimens is activated by preincubation with Mn2+ at 37 degrees C. After incubation with substrate at the same temperature, urea is determined fluorometrically by oxidation of NADH to NAD+ in a coupled kinetic reaction. We compare the results of this method with those of a colorimetric method involving liquid blood samples, and assess the stability of the enzyme in dried blood on filter paper. The presence of serum has no effect on the activity. This method may be useful in the early detection of arginase deficiency and certain hematological disorders.

scholarly journals Determination of Isepamicin in the Eluates from the Dried Blood Spots on Filter Paper for Monitoring of Blood Levels in a Guinea-pig

1989 ◽  
Vol 109 (12) ◽  
pp. 938-942 ◽  
Author(s):  
Koichiro KASE ◽  
Mayumi SHOSHIHARA ◽  
Yoshiko TSUDA ◽  
Eiko YOSHIZAWA ◽  
Takashi FUJIMOTO
Keyword(s):  
Guinea Pig ◽  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Levels ◽  
Blood Spots

A Phenytoin Assay Using Dried Blood Spot Samples Suitable for Domiciliary Therapeutic Drug Monitoring

1984 ◽  
Vol 21 (6) ◽  
pp. 519-522 ◽  
Author(s):  
E J Coombes ◽  
T R Gamlen ◽  
G F Batstone ◽  
P N Leigh
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Convenient Method ◽  
Filter Paper ◽  
Drug Monitoring ◽  
Dried Blood Spots ◽  
Therapeutic Drug ◽  
Plasma Samples ◽  
Blood Spots ◽  
Blood Spot

SUMMARY. A commercially available substrate-labelled fluorescent immunoassay procedure has been modified to create a simple rapid method for the determination of capillary phenytoin concentration in dried filter paper blood spots of patients on phenytoin therapy. The specimens are collected and despatched to the laboratory by the patient, thus domiciliary monitoring of phenytoin therapy can take place. The technique was validated by comparing the phenytoin results of simultaneously collected capillary dried blood spots and conventional plasma samples. The technique offers a convenient method for overcoming many of the practical problems of monitoring phenytoin therapy in epilepsy.

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