The Parallel Testing of Isolated Rat Liver and Kidney Mitochondria Reveals a Calcium-Dependent Sensitivity to Diclofenac and Ibuprofen

2018 ◽  
pp. 217-228
Author(s):  
Sabine Schulz ◽  
Sabine Borchard ◽  
Tamara Rieder ◽  
Carola Eberhagen ◽  
Bastian Popper ◽  
...  
Keyword(s):  
Rat Liver ◽  
Isolated Rat Liver ◽  
Kidney Mitochondria ◽  
Calcium Dependent ◽  
Parallel Testing ◽  
Liver And Kidney ◽  
Isolated Rat

Formation and metabolism of a glutathione-S-conjugate in isolated rat liver and kidney cells

1978 ◽  
Vol 83 (1) ◽  
pp. 195-200 ◽  
Author(s):  
Peter Moldèus ◽  
Dean P. Jones ◽  
Kari Ormstad ◽  
Sten Orrenius
Keyword(s):  
Rat Liver ◽  
Kidney Cells ◽  
Isolated Rat Liver ◽  
Liver And Kidney ◽  
Isolated Rat

BINDING OF L-TRIIODOTHYRONINE TO ISOLATED RAT LIVER AND KIDNEY NUCLEI UNDER VARIOUS CIRCUMSTANCES

1976 ◽  
Vol 81 (1) ◽  
pp. 82-95 ◽  
Author(s):  
R. Docter ◽  
T. J. Visser ◽  
J. T. Stinis ◽  
N. L. van den Hout-Goemaat ◽  
G. Hennemann
Keyword(s):  
Rat Liver ◽  
Binding Sites ◽  
Association Constant ◽  
Binding Capacity ◽  
Binding Protein ◽  
Isolated Rat Liver ◽  
Relative Affinity ◽  
Liver And Kidney ◽  
Maximal Binding ◽  
Isolated Rat

ABSTRACT Triiodothyronine (T3) is specifically bound by nuclei from rat liver and kidney. Binding of T3 at 37°C reaches its maximum at 30 min. In the presence of EDTA (to remove Mg2+) T3 binds to a class of binding sites with an estimated equilibrium association constant (Ka) of 3.3 × 1010 m−1 and a maximal binding capacity (MBC) of 3.8 fmol T3/100 μg DNA. In the presence of MgCl2 T3 binds to a second class of binding sites with an estimated Ka of 4.8 × 108 m−1 and a MBC of 123 fmol/100 μg DNA. Among the analogues tested, binding - relative to T3 - of D-T3, triiodothyroacetic acid and desamino-T3 is the same for both classes of binding sites, but relative affinity of thyroxine, D-T4, tetraiodothyroacetic acid and desamino-T4 is 2.5 to 6 times higher for the second class of binding sites than for the first class. The presence of a cytosol binding protein is not a prerequisite for binding of T3 to the nucleus.

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