FluorescenceIn Situ Hybridization: Applications in Gene Mapping and Clinical Diagnostics

2008 ◽  
pp. 25-44
Author(s):  
G. A. Evans ◽  
L. Selleri
Author(s):  
W. Bernard

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.


Author(s):  
Arndt Rolfs ◽  
Irmela Schuller ◽  
Ulrich Finckh ◽  
Ines Weber-Rolfs
Keyword(s):  

Pathology ◽  
1984 ◽  
Vol 16 (1) ◽  
pp. 16-21 ◽  
Author(s):  
Ronald J. Trent ◽  
P.E. Brock ◽  
J. Yakas ◽  
L.M. Trent ◽  
H. Kronenberg
Keyword(s):  

MedAlliance ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 14-24

The clinic and diagnostics of tuberculosis meningitis (TM) in 926 patients treated in St. Petersburg hospitals in 1965–1994 (group 1) and in 1995–2018 (group 2) is presented. The TM clinic is demonstrated to be determined by the form of tuberculosis and its characteristic generalization nature in the presence of repeated waves of bacteremia and allergic vasculitis of greater or lesser severity. There is clinical peculiarity of TM in primary pulmonary tuberculosis and its early large-focal and late miliar generalization, as well as in hematogenous tuberculosis. In patients of the 1st and 2nd groups the TM clinic shows in some respects a noticeable similarity, in others — a significant difference. Despite the typical symptoms, early diagnosis of TM took place in less than 20% of patients. Clinical examples illustrating the unusual development of TM, contrasting with its usual course, are given. A number of objective and subjective factors contributing to the adverse evolution of TM and its lethal outcome are discussed. These include the peculiarity of modern tuberculosis, especially when associated with HIV infection, as well as medical errors associated with ignorance of the pathogenesis of tuberculosis and failure to comply with the minimum examination for tuberculosis.


2020 ◽  
Vol 62 (1) ◽  
pp. 55-59
Author(s):  
Krzysztof Mataczyński ◽  
Mateusz Pelc ◽  
Halina Romualda Zięba ◽  
Zuzana Hudakova

Acquired adult flatfoot is a three-dimensional deformation, which consists of hindfoot valgus, collapse of the longitudinal arch of the foot and adduction of the forefoot. The aim of the work is to present problems related to etiology, biomechanics, clinical diagnostics and treatment principles of acquired flatfoot. The most common cause in adults is the dysfunction of the tibialis posterior muscle, leading to the lack of blocking of the transverse tarsal joint during heel elevation. Loading the unblocked joints consequently leads to ligament failure. The clinical image is dominated by pain in the foot and tibiotarsal joint. The physical examination of the flat feet consists of: inspection, palpation, motion range assessment and dynamic force assessment. The comparable attention should be paid to the height of the foot arch, the occurrence of “too many toes” sign, evaluate the heel- rise test and correction of the flatfoot, exclude Achilles tendon contracture. The diagnosis also uses imaging tests. In elastic deformations with symptoms of posterior tibial tendonitis, non-steroidal anti-inflammatory drugs, short-term immobilization, orthotics stabilizing the medial arch of the foot are used. In rehabilitation, active exercises of the shin muscles and the feet, especially the eccentric exercises of the posterior tibial muscle, are intentional. The physiotherapy and balneotherapy treatments, in particular hydrotherapy, electrotherapy and laser therapy, are used as a support. In advanced lesions, surgical treatment may be necessary, including plastic surgery of soft tissues, tendons, as well as osteotomy procedures.


2018 ◽  
Vol 44 (2) ◽  
pp. 169
Author(s):  
Xian-Cheng YAN ◽  
Li-Kai CHEN ◽  
Yu-Hua LUO ◽  
Wen-Long LUO ◽  
Hui WANG ◽  
...  

2011 ◽  
Vol 37 (2) ◽  
pp. 280-285
Author(s):  
Yun-Feng LI ◽  
Zheng-Lin YANG ◽  
Ying-Hua LING ◽  
Nan WANG ◽  
De-Yong REN ◽  
...  
Keyword(s):  

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