Integrase (integrase protein)

ABSTRACT The integrase protein of the Rhizobium meliloti 41 phage 16-3 has been classified as a member of the Int family of tyrosine recombinases. The site-specific recombination system of the phage belongs to the group in which the target site of integration (attB) is within a tRNA gene. Since tRNA genes are conserved, we expected that the target sequence of the site-specific recombination system of the 16-3 phage could occur in other species and integration could take place if the required putative host factors were also provided by the targeted cells. Here we report that a plasmid (pSEM167) carrying the attP element and the integrase gene (int) of the phage can integrate into the chromosomes of R. meliloti 1021 and eight other species. In all cases integration occurred at so-far-unidentified, putative proline tRNA (CGG) genes, indicating the possibility of their common origin. Multiple alignment of the sequences suggested that the location of the att core was different from that expected previously. The minimal attB was identified as a 23-bp sequence corresponding to the anticodon arm of the tRNA.

Download Full-text

Sip, an integrase protein with excision, circularization and integration activities, defines a new family of mobile Staphylococcus aureus pathogenicity islands

Molecular Microbiology ◽

10.1046/j.1365-2958.2003.03577.x ◽

2003 ◽

Vol 49 (1) ◽

pp. 193-210 ◽

Cited By ~ 79

Author(s):

Carles Úbeda ◽

Ma. Ángeles Tormo ◽

Carme Cucarella ◽

Pilar Trotonda ◽

Timothy J. Foster ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pathogenicity Islands ◽

New Family ◽

Integrase Protein

Download Full-text

Structural Studies of the HIV-1 Integrase Protein: Compound Screening and Characterization of a DNA-Binding Inhibitor

PLoS ONE ◽

10.1371/journal.pone.0128310 ◽

2015 ◽

Vol 10 (6) ◽

pp. e0128310 ◽

Cited By ~ 11

Author(s):

Peter K. Quashie ◽

Ying-Shan Han ◽

Said Hassounah ◽

Thibault Mesplède ◽

Mark A. Wainberg

Keyword(s):

Dna Binding ◽

Structural Studies ◽

Compound Screening ◽

Hiv 1 ◽

Integrase Protein

Download Full-text

Specific Binding of Integrase to the Origin of Transfer (oriT) of the Conjugative Transposon Tn916

Journal of Bacteriology ◽

10.1128/jb.183.9.2947-2951.2001 ◽

2001 ◽

Vol 183 (9) ◽

pp. 2947-2951 ◽

Cited By ~ 15

Author(s):

Douglas Hinerfeld ◽

Gordon Churchward

Keyword(s):

Dna Binding ◽

Specific Binding ◽

Binding Domain ◽

Conjugal Transfer ◽

Dna Binding Domain ◽

Conjugative Transposon ◽

Dna Binding Domains ◽

Binding Domains ◽

A Site ◽

Integrase Protein

ABSTRACT Purified integrase protein (Int) of the conjugative transposon Tn916 was shown, using nuclease protection experiments, to bind specifically to a site within the origin of conjugal transfer of the transposon, oriT. A sequence similar to the ends of the transposon that are bound by the C-terminal DNA-binding domain of Int was present in the protected region. However, Int binding tooriT required both the N- and C-terminal DNA-binding domains of Int, and the pattern of nuclease protection differed from that observed when Int binds to the transposon ends and flanking DNA. Binding of Int to oriT may be part of a mechanism to prevent premature conjugal transfer of Tn916 prior to excision from the donor DNA.

Download Full-text