In Vitro and In Vivo Evaluation of Whitlockite Biocompatibility: Comparative Study with Hydroxyapatite andβ-Tricalcium Phosphate

A biodegradable polylactic acid composite containing tricalcium phosphate microsphere was fabricated. The composite exhibited enhanced biocompatibility and a well-interconnected porous structure that enabled tissue ingrowth after degradation. The tricalcium phosphate microspheres had an average size of 106 ± 43 μm and were incorporated into the polylactic acid matrix using a high-shear mixer. The resulting bioactivity and hydrophilicity were enhanced to levels comparable to those of a polylactic acid composite containing tricalcium phosphate powder, which is a well-known material used in the medical field. An accelerated 30-day degradation test in HCl revealed successful generation of an open porous structure with ∼98% interconnectivity in the polylactic acid–tricalcium phosphate microsphere composite, demonstrating the potential of this material to induce enhanced osseointegration in the later stage of bone regeneration. The early stage osseointegration was also evaluated by implanting the composite in vivo using a rabbit femoral defect model. After 16 weeks of implantation, the bone-to-implant contact ratio of the polylactic acid–tricalcium phosphate microsphere composite was enhanced owing to tissue ingrowth through the generated pores near the surface.

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A comparative study of wound dressings loaded with silver sulfadiazine and silver nanoparticles: In vitro and in vivo evaluation

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2019.04.068 ◽

2019 ◽

Vol 564 ◽

pp. 350-358 ◽

Cited By ~ 17

Author(s):

Mina Mohseni ◽

Amir Shamloo ◽

Zahra Aghababaie ◽

Homa Afjoul ◽

Shabnam Abdi ◽

...

Keyword(s):

Silver Nanoparticles ◽

Comparative Study ◽

Silver Sulfadiazine ◽

Wound Dressings ◽

In Vivo Evaluation

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Polycaprolactone/Beta-Tricalcium Phosphate Scaffolds Obtained via Rotary Jet-Spinning: in vitro and in vivo Evaluation

Cells Tissues Organs ◽

10.1159/000511570 ◽

2021 ◽

pp. 1-15

Author(s):

Stella Aparecida de Andrade Pinto ◽

Francisco José de Nadai Dias ◽

Guinea Brasil Camargo Cardoso ◽

Arnaldo Rodrigues dos Santos Junior ◽

Andréa Aparecida de Aro ◽

...

Keyword(s):

Tricalcium Phosphate ◽

Polymeric Membranes ◽

Experimental Sample ◽

New Bone Formation ◽

In Vivo Evaluation ◽

Beta Tricalcium Phosphate ◽

Spinning Process ◽

Critical Bone Defects

This study aimed to evaluate in vitro and in vivo polymeric membranes obtained by a rotary jet-spinning process for the repair of critical bone defects in the calvaria of Wistar rats, for future use in tissue engineering. Experimental sample collections were performed on the 30, 60 and 90th postoperative days, and the analyses performed were histomorphometric, immunohistochemistry, and western blotting. Reducing inflammatory infiltrate in all groups and experimental periods, angiogenesis on the 30th day did not show any difference between the groups, on the 60th day, 5% polycaprolactone/beta-tricalcium phosphate(PCL/β-TCP) was high compared to control (C), and on the 90th day, the same group reduced when compared to C and 10% PCL/β-TCP. The fibroplasia presented oscillations in every segment; on the 30th and 60th day, there was an increase in 5% PCL/β-TCP, which decreased by the 90th day compared to group C. 10% PCL/β-TCP decreased compared to C on the 60th and 90th day. The percentage of the collagen area remained high in all groups and all experimental periods. Immunohistochemistry quantifications showed variations in bone metabolism suggesting new bone formation. The 5 and 10% PCL/β-TCP scaffold were promising for the bone regeneration process because they participated in the modulation of inflammation, angiogenesis, fibroplasia, and collagenosis.

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