In vitro and in vivo evaluation of polylactic acid-based composite with tricalcium phosphate microsphere for enhanced biodegradability and osseointegration

2018 ◽  
Vol 32 (10) ◽  
pp. 1360-1370 ◽  
Author(s):  
Da Yong Shin ◽  
Min-Ho Kang ◽  
In-Gu Kang ◽  
Hyoun-Ee Kim ◽  
Seol-Ha Jeong
Keyword(s):  
Porous Structure ◽  
Polylactic Acid ◽  
Tricalcium Phosphate ◽  
Early Stage ◽  
Contact Ratio ◽  
In Vivo Evaluation ◽  
Tissue Ingrowth ◽  
Average Size

A biodegradable polylactic acid composite containing tricalcium phosphate microsphere was fabricated. The composite exhibited enhanced biocompatibility and a well-interconnected porous structure that enabled tissue ingrowth after degradation. The tricalcium phosphate microspheres had an average size of 106 ± 43 μm and were incorporated into the polylactic acid matrix using a high-shear mixer. The resulting bioactivity and hydrophilicity were enhanced to levels comparable to those of a polylactic acid composite containing tricalcium phosphate powder, which is a well-known material used in the medical field. An accelerated 30-day degradation test in HCl revealed successful generation of an open porous structure with ∼98% interconnectivity in the polylactic acid–tricalcium phosphate microsphere composite, demonstrating the potential of this material to induce enhanced osseointegration in the later stage of bone regeneration. The early stage osseointegration was also evaluated by implanting the composite in vivo using a rabbit femoral defect model. After 16 weeks of implantation, the bone-to-implant contact ratio of the polylactic acid–tricalcium phosphate microsphere composite was enhanced owing to tissue ingrowth through the generated pores near the surface.

Formation and In Vitro and In Vivo Evaluation of Surface Modified Implants by HAp, Carbonated-HAp and Titanium Oxide

2007 ◽  
Vol 539-543 ◽  
pp. 687-691 ◽  
Author(s):  
Masazumi Okido ◽  
Ryoichi Ichino ◽  
Kotaro Kuroda
Keyword(s):  
Titanium Oxide ◽  
Titanium Implant ◽  
Titanium Implants ◽  
Contact Ratio ◽  
In Vivo Evaluation ◽  
Ft Ir ◽  
Mouse Osteoblast ◽  
Surface Modified

Hydroxyapatite (Ca10(PO4)6(OH)2, HAp), carbonated HAp and titanium oxide are of interest for bone-interfacing implant applications, because of their demonstrated osteoconductive properties. They were coated on the titanium implants and investigated the in vitro and in vivo performance. HAp coatings were performed by the thermal substrate method in aqueous solutions. Titanium oxide film was also formed on the titanium implants by gaseous oxidation, or by anodizing in the acidic solution. All the specimens covered with HAp, carbonated HAp or TiO2 (rutile or anatase). were characterized by XRD, EDX, FT-IR and SEM. In the in vitro testing, the mouse osteoblast-like cells (MC3T3-E1) were cultured on the coated and non-coated specimens for up to 30 days. Moreover, the osseointegration was evaluated from the rod specimens implanted in rats femoral for up to 8 weeks. In in vivo evaluations two weeks postimplantation, new bone formed on the coated and non-coated titanium rods in the cancellous bone and cortical bone, respectively. Bone-implant contact ratio, in order to evaluate of new bone formation, was significantly depended on the compound formed on the titanium implant.

Improvement in osseointegration of tricalcium phosphate-zircon for orthopedic applications: an in vitro and in vivo evaluation

2020 ◽  
Vol 58 (8) ◽  
pp. 1681-1693
Author(s):  
Abolfazl Bagherifard ◽  
Hamed Joneidi Yekta ◽  
Hossein Akbari Aghdam ◽  
Mehdi Motififard ◽  
Ehsan Sanatizadeh ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
In Vivo Evaluation ◽  
Orthopedic Applications

Polycaprolactone/Beta-Tricalcium Phosphate Scaffolds Obtained via Rotary Jet-Spinning: in vitro and in vivo Evaluation

2021 ◽  
pp. 1-15
Author(s):  
Stella Aparecida de Andrade Pinto ◽  
Francisco José de Nadai Dias ◽  
Guinea Brasil Camargo Cardoso ◽  
Arnaldo Rodrigues dos Santos Junior ◽  
Andréa Aparecida de Aro ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Polymeric Membranes ◽  
Experimental Sample ◽  
New Bone Formation ◽  
In Vivo Evaluation ◽  
Beta Tricalcium Phosphate ◽  
Spinning Process ◽  
Critical Bone Defects

This study aimed to evaluate in vitro and in vivo polymeric membranes obtained by a rotary jet-spinning process for the repair of critical bone defects in the calvaria of Wistar rats, for future use in tissue engineering. Experimental sample collections were performed on the 30, 60 and 90th postoperative days, and the analyses performed were histomorphometric, immunohistochemistry, and western blotting. Reducing inflammatory infiltrate in all groups and experimental periods, angiogenesis on the 30th day did not show any difference between the groups, on the 60th day, 5% polycaprolactone/beta-tricalcium phosphate(PCL/β-TCP) was high compared to control (C), and on the 90th day, the same group reduced when compared to C and 10% PCL/β-TCP. The fibroplasia presented oscillations in every segment; on the 30th and 60th day, there was an increase in 5% PCL/β-TCP, which decreased by the 90th day compared to group C. 10% PCL/β-TCP decreased compared to C on the 60th and 90th day. The percentage of the collagen area remained high in all groups and all experimental periods. Immunohistochemistry quantifications showed variations in bone metabolism suggesting new bone formation. The 5 and 10% PCL/β-TCP scaffold were promising for the bone regeneration process because they participated in the modulation of inflammation, angiogenesis, fibroplasia, and collagenosis.

In Vitro and In Vivo Evaluation of Whitlockite Biocompatibility: Comparative Study with Hydroxyapatite andβ-Tricalcium Phosphate

2015 ◽  
Vol 5 (1) ◽  
pp. 128-136 ◽  
Author(s):  
Hae Lin Jang ◽  
Guang Bin Zheng ◽  
Jungha Park ◽  
Hwan D. Kim ◽  
Hae-Ri Baek ◽  
...  
Keyword(s):  
Comparative Study ◽  
Tricalcium Phosphate ◽  
In Vivo Evaluation

scholarly journals A Combined Computer-Aided Approach to Drive the Identification of Potential Epitopes in Protein Therapeutics

2018 ◽  
Vol 21 ◽  
pp. 268-285 ◽  
Author(s):  
Bárbara Athayde Vaz Galvão da Silva ◽  
Ana Marisa Chudzinski-Tavassi ◽  
Kerly Fernanda Mesquita Pasqualoto
Keyword(s):  
T Cells ◽  
Early Stage ◽  
3D Structure ◽  
Cell Epitope ◽  
Protein Therapeutics ◽  
In Vivo Evaluation ◽  
Computer Aided ◽  
Kunitz Type

Background: The identification of fragment sequences, or motifs, within a therapeutic protein that may elicit an immune response when processed by T-cells can be provided by computer-aided approaches. Immunogenicity is a significant problem associated with protein therapeutics and should be investigated in the early stage of protein-based drug development to avoid treatment resistance and potentially life-threatening immune responses. Purpose: To provide a combined computer-aided protocol for investigating the immunogenic profile of a recombinant Kunitz-type inhibitor, which has been reported as promising antitumor agent by our research group. Methods: The combination of databases searching (IEDB and SYFPEITHI) and molecular docking simulations was exploited, herein. This combined protocol has allowed the identification of potential epitopes before in vitro/in vivo evaluation. Predictors of human proteasome cleavage transport and major histocompatibility complex (MHC) binding were considered as overall score assigning the corresponding intrinsic potential of being a T cell epitope to each fragment sequence. The peptides or motifs better classified in the two databases were docked into the three-dimensional (3D) structure of MHC (class I and II) complex to verify the calculated binding affinity.  The binding interactions regarding the molecular recognition process by T-cells were also exploited through the MHC:ligand:T-cell complexes. Results: Regarding the Kunitz-type sequence, four motifs were identified as potentially epitopes for MHC-I and three motifs were found for MHC-II. But, those motifs were classified as moderately immunogenic. Final remarks: The combined computer-aided protocol has significantly reduced the number of potential epitopes to be considered for further analysis and could be useful to identify immunogenic fragments (high, moderate and low) in protein pharmaceutics before in vitro/in vivo experimentation.

scholarly journals In Vitro and In Vivo Evaluation of Starfish Bone-Derived β-Tricalcium Phosphate as a Bone Substitute Material

Materials ◽  
2019 ◽  
Vol 12 (11) ◽  
pp. 1881 ◽  
Author(s):  
Haruka Ishida ◽  
Hisao Haniu ◽  
Akari Takeuchi ◽  
Katsuya Ueda ◽  
Mahoko Sano ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Bone Substitute ◽  
Fibrous Tissue ◽  
Bone Substitute Material ◽  
In Vivo Evaluation ◽  
Tissue Invasion ◽  
Substitute Material ◽  
Porous Calcium Carbonate

We evaluated starfish-derived β-tricalcium phosphate (Sf-TCP) obtained by phosphatization of starfish-bone-derived porous calcium carbonate as a potential bone substitute material. The Sf-TCP had a communicating pore structure with a pore size of approximately 10 μm. Although the porosity of Sf-TCP was similar to that of Cerasorb M (CM)—a commercially available β-TCP bone filler—the specific surface area was roughly three times larger than that of CM. Observation by scanning electron microscopy showed that pores communicated to the inside of the Sf-TCP. Cell growth tests showed that Sf-TCP improved cell proliferation compared with CM. Cells grown on Sf-TCP showed stretched filopodia and adhered; cells migrated both to the surface and into pores. In vivo, vigorous tissue invasion into pores was observed in Sf-TCP, and more fibrous tissue was observed for Sf-TCP than CM. Moreover, capillary formation into pores was observed for Sf-TCP. Thus, Sf-TCP showed excellent biocompatibility in vitro and more vigorous bone formation in vivo, indicating the possible applications of this material as a bone substitute. In addition, our findings suggested that mimicking the microstructure derived from whole organisms may facilitate the development of superior artificial bone.

An assessment of in-vitro and in-vivo evaluation methods for theranostic nanomaterials

2021 ◽  
Vol 27 ◽  
Author(s):  
Poonam Parashar ◽  
Neelu Singh ◽  
Alka Alka ◽  
Priyanka Maurya ◽  
Shubhini A. Saraf
Keyword(s):  
Surface Engineering ◽  
Early Stage ◽  
Evaluation Criteria ◽  
In Vivo Evaluation ◽  
Size Dependent ◽  
Entry Points ◽  
Theranostic Agents ◽  
Diagnostic Agent

: Nanoparticles (NPs) as nanocarriers have emerged as novel and promising theranostic agents. The term theranostics revealed the properties of NPs capable of diagnosing the disease at an early stage and/or treating the disease. Such NPs are usually developed employing a surface engineering approach. The theranostic agents comprise NPs loaded with a drug/diagnostic agent that delivers it precisely to the target site. Theranostics is a field with promising results in enhancing therapeutic efficacy facilitated through higher payload at the targeted tissue, reduced dose, and dose-dependent side effects. However, controversies in terms of toxicity and size-dependent properties have often surfaced for NPs. Thus, a stringent in-vitro and in-vivo evaluation is required to develop safe and non-toxic NPs as theranostic agents. The review also focuses on the various entry points of NPs in the human system and their outcomes, including toxicity. It elaborates the evaluation criteria to ensure the safe use of NPs for diagnostic and therapeutic purposes.

In vitro and in vivo evaluation of the anti-inflammatory effects of Arzanol from Helichrysum italicum

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
J Bauer ◽  
F Dehm ◽  
A Koeberle ◽  
F Pollastro ◽  
G Appendino ◽  
...  
Keyword(s):  
In Vivo Evaluation ◽  
Anti Inflammatory
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