Electric-Field-Assisted Single-Step In Situ Fabrication and Focal Length Control of Polymeric Convex Lens on Flexible Substrate

2018 ◽  
Vol 3 (11) ◽  
pp. 1800108 ◽  
Author(s):  
Jonghyun Kim ◽  
Dongwoon Shin ◽  
Tawsif Mahmood ◽  
Jiyoung Chang
Keyword(s):  
Electric Field ◽  
Focal Length ◽  
Flexible Substrate ◽  
Single Step ◽  
In Situ Fabrication ◽  
Convex Lens

Facile Single-step Technique for Simultaneous In Situ Fabrication of Ag/Ag2S–Polymer Nanocomposites via PPS Cyclization Route

2008 ◽  
Vol 37 (2) ◽  
pp. 194-195 ◽  
Author(s):  
Sujata Waghmare ◽  
Manish Shinde ◽  
Ramkrishna Gholap ◽  
N. Koteswara Rao ◽  
Tanay Seth ◽  
...  
Keyword(s):  
Polymer Nanocomposites ◽  
Single Step ◽  
In Situ Fabrication

In-situ fabrication of an out-of-plane microlens with pre-definable focal length

2013 ◽  
Vol 19 (11) ◽  
pp. 1823-1828 ◽  
Author(s):  
Guocheng Shao ◽  
Ziliang Cai ◽  
Zhengyu Miao ◽  
Wanjun Wang
Keyword(s):  
Focal Length ◽  
In Situ Fabrication ◽  
Out Of Plane

Electric Field-Driven Interfacial Alloying for in Situ Fabrication of Nano-Mo2C on Carbon Fabric as Cathode toward Efficient Hydrogen Generation

2019 ◽  
Vol 11 (42) ◽  
pp. 38606-38615 ◽  
Author(s):  
Rui Jiang ◽  
Liu Pi ◽  
Bowen Deng ◽  
Liangyou Hu ◽  
Xianglin Liu ◽  
...  
Keyword(s):  
Electric Field ◽  
Hydrogen Generation ◽  
Carbon Fabric ◽  
In Situ Fabrication

scholarly journals Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Single Step ◽  
Flow Cytometer ◽  
Surface Proteins ◽  
Biomarker Detection ◽  
In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

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