scholarly journals Characterization of direct antiglobulin test-negative autoimmune hemolytic anemia: A study of 154 cases

2012 ◽  
Vol 88 (2) ◽  
pp. 93-96 ◽  
Author(s):  
Toyomi Kamesaki ◽  
Tomonori Toyotsuji ◽  
Eiji Kajii
Keyword(s):  
Hemolytic Anemia ◽  
Autoimmune Hemolytic Anemia ◽  
Direct Antiglobulin Test ◽  
Antiglobulin Test

Direct antiglobulin test reactive with complement only in warm type autoimmune hemolytic anemia

2007 ◽  
Vol 0 (0) ◽  
pp. 071003000343002-???
Author(s):  
L. SHVIDEL ◽  
M. SHTALRID ◽  
A. DUEK ◽  
M. HARAN ◽  
A. BERREBI ◽  
...  
Keyword(s):  
Hemolytic Anemia ◽  
Autoimmune Hemolytic Anemia ◽  
Direct Antiglobulin Test ◽  
Antiglobulin Test

scholarly journals Warm Autoimmune Hemolytic Anemia with a Direct Antiglobulin Test Positive for C3 and Negative for IgG: A Case Study and Analytical Literature Review of Incidence and Severity

2013 ◽  
Vol 6 ◽  
pp. CCRep.S11469 ◽  
Author(s):  
Amruth R. Palla ◽  
Farhad Khimani ◽  
Michael D. Craig
Keyword(s):  
Literature Review ◽  
Hemolytic Anemia ◽  
Autoimmune Hemolytic Anemia ◽  
Interesting Case ◽  
Clinical Entity ◽  
Direct Antiglobulin Test ◽  
Complement C3 ◽  
Igg Antibodies ◽  
Antiglobulin Test

Polygenic IgG autoantibodies are implicated in majority of the cases of warm autoimmune hemolytic anemia (WAIHA). In some of these cases, complement (C3) proteins accompany the IgG antibodies. WAIHA mediated by C3 alone is relatively rare. We present an interesting case of WAIHA with a direct antiglobulin test (DAT) positive for C3 but negative for IgG in a 79-year-old woman and perform an analytical literature review of the incidence and severity of this clinical entity.

A Comprehensive Diagnostic Algorithm for Direct Antiglobulin Test-Negative Autoimmune Hemolytic Anemia Reveals the Relative Ratio of Three Mechanisms in a Single Laboratory

2018 ◽  
Vol 140 (1) ◽  
pp. 10-17 ◽  
Author(s):  
Toyomi Kamesaki ◽  
Eiji Kajii
Keyword(s):  
Hemolytic Anemia ◽  
Autoimmune Hemolytic Anemia ◽  
Diagnostic Algorithm ◽  
Direct Antiglobulin Test ◽  
New Classification ◽  
Antiglobulin Test ◽  
Agglutination Method ◽  
Before And After ◽  
Ig G ◽  
Single Laboratory

Background: Direct antiglobulin test (DAT)-negative warm autoimmune hemolytic anemia (AIHA) is mainly caused by three mechanisms: red blood cell (RBC)-bound immunoglobulin (Ig)G below the detection limit of routine DAT; RBC-bound IgA or IgM; or low-affinity autoantibodies. Although most cases of DAT-negative AIHA are thought to be caused by RBC-bound IgG, and combinatory serological analyses are recommended, the relative ratios of each mechanism have not been clarified. Methods: Two groups of patients with undiagnosed hemolytic anemia and negative conventional tube method-DAT (TM-DAT) were investigated using anti-IgA and anti-IgM sera, or column agglutination method-DAT (CM-DAT), respectively, in addition to radioimmunological quantitation of RBC-bound IgG. Results: Three of 73 patients with DAT-negative AIHA showed positive RBC-bound IgA and normal amounts of RBC-bound IgG. Another group of 3 patients were RBC-bound IgM-positive, but only one of these showed normal amounts of RBC-bound IgG. In another group of patients with DAT-negative AIHA, 4 of the 20 showed positive CM-DAT and negative CM-DAT after washing RBCs. Three of these patients had normal amounts of RBC-bound IgG. Five patients with positive CM-DAT both before and after washing RBCs had high amounts of RBC-bound IgG. Conclusion: Relative ratios of patients with DAT-negative AIHA resulting from RBC-bound IgG, RBC-bound IgA, RBC-bound IgM, and low-affinity IgG were estimated as 80, 4, 1 and 15%, respectively. A new classification and diagnostic algorithm for DAT-negative AIHA were proposed.

scholarly journals COVID-19-ASSOCIATED DIRECT ANTIGLOBULIN TEST-NEGATIVE WARM AUTOIMMUNE HEMOLYTIC ANEMIA

CHEST Journal ◽  
2021 ◽  
Vol 160 (4) ◽  
pp. A414
Author(s):  
Tien-Chan Hsieh ◽  
Russell Lewis ◽  
Stephanie Halene
Keyword(s):  
Hemolytic Anemia ◽  
Autoimmune Hemolytic Anemia ◽  
Direct Antiglobulin Test ◽  
Antiglobulin Test

scholarly journals Coomb’s Test Negative Autoimmune Hemolytic Anemia

2015 ◽  
Vol 12 (2) ◽  
pp. 54-57
Author(s):  
Ajaya Kumar Dhakal ◽  
Devendra Shrestha ◽  
Subhash Chandra Shah
Keyword(s):  
Vitamin B12 ◽  
Hemolytic Anemia ◽  
Autoimmune Hemolytic Anemia ◽  
Serum Vitamin ◽  
Oral Prednisolone ◽  
Direct Antiglobulin Test ◽  
Severe Anemia ◽  
Antiglobulin Test ◽  
High Degree

Autoimmune hemolytic anemia is uncommon type of anemia in children. High degree of clinical suspicionalong with detailed investigations is required for diagnosis. It is characterized by anemia and positive directantiglobulin test along with features of hemolysis. Infrequently direct antiglobulin test may be negative inautoimmune hemolytic anemia and may pose diagnostic difÞ culty. We report a 14 year-old female with twoacute episodes of severe anemia along with splenomegaly, reticulocytosis, unconjugated hyperbilrubinemia andnegative direct antiglobulin test. She was successfully treated with oral prednisolone. Serum Vitamin B12 levelwas low in this patient and association between autoimmune hemolytic anemia and Vitamin B12 deÞ ciencycould not be explained.doi: http://dx.doi.org/10.3126/mjsbh.v12i2.12930

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