agglutination method
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2021 ◽  
Vol 12 ◽  
Author(s):  
Zuzana Kubicová ◽  
Sophie Roussel ◽  
Benjamin Félix ◽  
Lenka Cabanová

Over the past 11 years, the Slovak National Reference Laboratory has collected a panel of 988 Listeria monocytogenes isolates in Slovakia, which were isolated from various food sectors (61%), food-processing environments (13.7%), animals with listeriosis symptoms (21.2%), and human cases (4.1%). We serotyped these isolates by agglutination method, which revealed the highest prevalence (61.1%) of serotype 1/2a and the lowest (4.7%) of serotype 1/2c, although these represented the majority of isolates from the meat sector. The distribution of CCs analyzed on 176 isolates demonstrated that CC11-ST451 (15.3%) was the most prevalent CC, particularly in food (14.8%) and animal isolates (17.5%). CC11-ST451, followed by CC7, CC14, and CC37, were the most prevalent CCs in the milk sector, and CC9 and CC8 in the meat sector. CC11-ST451 is probably widely distributed in Slovakia, mainly in the milk and dairy product sectors, posing a possible threat to public health. Potential persistence indication of CC9 was observed in one meat facility between 2014 and 2018, highlighting its general meat-related distribution and potential for persistence worldwide.


2021 ◽  
Vol 19 (3) ◽  
pp. 529-537
Author(s):  
Le Thi Lan Anh ◽  
Vu Thi Thuong ◽  
Pham Thi Ha Giang ◽  
Bui Thi Thanh Nga ◽  
Minh Thi Hang ◽  
...  

Leptospirosis is a common zoonotic disease in the tropics and subtropics. Leptospira infected human without prompt detection and treatment will face serious consequences such as acute hepatitis-kidneys, pulmonary hemorrhage which can lead to death. Besides the MAT gold standard method, Leptosipra antigens developed by DNA recombination technology have been widely studying and applying in diagnosis of Leptospira infection in human and animal. Overcoming the disadvantages of MAT and ELISA such as complicated protocol, which requires highly qualified staff and specialized equipment, the latex agglutination method has been studied and widely used in detecting pathogens such as Salmonella, E. coli, Leptospira in the world. The advantages of this method are simple operation, fast and cheap. In the previous article, we expressed Leptospira LigB antigen in E. coli cells and successfully purified it by affinity chromatography with 98% purity. In this paper, we present the process for establishment of a Lepto-LAT kit to detect Leptospira infection in dogs. This kit had the sensitivity and specificity of 91.75% and 91.57%, respectively.


Author(s):  
M.L Avinash Tejasvi ◽  
Jaya Laksmi Bukkya ◽  
Pandu Ranga Rao ◽  
Harsha Bhayya

Abstract Objectives While DNA profiling has become the principal technique for individualization of biological evidences, ABO blood grouping is still a useful test method in the initial stages of crime investigation. Objectives of the study were blood group determination using slide agglutination method, blood group determination from saliva using absorption inhibition method, and comparison of slide agglutination method with that of absorption inhibition method from saliva sample. Materials and Methods A total of 60 subjects were taken randomly with their age ranging from 20 to 60 years. Sixty subjects were divided in to two groups, study group and control group. 5 to 10 mL of unstimulated saliva was collected from 60 patients and Wieners agglutination test was performed to detect the secretor status of blood using absorption inhibition method and compared with that of slide agglutination method Results Out of 60 subjects, 52 subjects showed secretors of antigen in saliva with percentage value of 86.66% and eight subjects were nonsecretors (13.33%). Slightly higher percentage of secretor status was seen in males 84.6 and 88.2% in females. Conclusion Evaluation of secretor status of blood group antigen from saliva using absorption inhibition method can be useful method in identification of medicolegal cases.


2020 ◽  
Vol 27 (07) ◽  
pp. 1363-1370
Author(s):  
Aneela Khawaja ◽  
Iffat Javed ◽  
Sohaila Mushtaq ◽  
Saeed Anwar ◽  
Faiqa Arshad ◽  
...  

Antimicrobial resistance (AMR) is a devastating question that is threatening the health globally. The extensive and indiscriminative use of antibiotics has evolved a notorious resistance in Staphylococcus aureus.  This resistance developed through possession of mecA gene, which codes for modified penicillin binding protein (PBP2a) and the emergent strain being labeled “methicillin resistant Staphylococcus aureus”. Conventional phenotypic techniques for detection of MRSA rely on standardization of cultural characteristics. The drawbacks of diagnostic error to report MRSA include: poor prognosis, expensive treatment, dissemination of multi-drug resistant strains and even treatment failure. Latex agglutination method can be adopted as a more accurate and quick strategy for rapid detection of methicillin resistance. Objectives: To compare detection of mecA gene in methicillin resistant isolates of Staphylococcus aureus by latex agglutination and PCR; by assessing the sensitivity and specificity of both methods. Study Design: Descriptive Cross-Sectional study. Setting: Pathology Department, Post Graduate Medical Institute, Lahore. Period: From January 2015 to December 2015; according to standard operating procedures at Microbiology laboratory. Material & Methods: A total 713 consecutive, non-duplicate isolates of Staphylococcus aureus were processed. Methicillin resistance was determined using cefoxitin (30mg) by Kirby-Bauer method using CLSI guideline (2016), latex agglutination method; and PCR for mecA gene. Results: The results showed that out of 713 Staphylococcus aureus isolates, 92 (12.90%) isolates were resistant to cefoxitin and were labelled as MRSA. majority MRSA isolates recovered from pus (44.57%) and wound swab (20.65%), followed by blood (13.04%), fluid (8.70%), CSF (4.35%), CVP (3.26%), HVS (3.26%) and tracheal secretion (2.17%). By latex agglutination method, 87 (94.50%) were positive for PBP2a; while on PCR mecA gene was detected only in 82 (89.10%) MRSA isolates. When assessed with PCR (gold standard) the sensitivity and diagnostic accuracy of latex agglutination was 100% and 94.57%, respectively. Conclusion: Latex agglutination test can be employed as rapid and reliable diagnostic technique in MRSA isolates for mecA gene detection, where resources for molecular methods are inadequate. This can effectually lessen the misdiagnosis of resistant strains, and over/ ill-use of antibiotics.


2020 ◽  
Vol 10 (02) ◽  
pp. 307-310
Author(s):  
Yousor Majid Jameel ◽  
Rana S. Nasir

This work was done during the period from 1st May 2019 to 15th January 2020. By using the concentration of the inhibitor that was carried out for the Haemophilus influenza bacteria, isolates were identified that 50 samples of bacterial colonies of these bacteria, it was found that all isolates were resistant to ampicillin, cefuroxime, pipracilline, cefotoxone, erythromycin, and azithromycin. The counter-current immunoelectrophoresis (CCIE) test and the latex agglutination method have been employed for this purpose. These tests have aided in establishing a rapid diagnosis of H. influenzae type b infections in some cases, the positive isolates were identified for those infected with the bacteria were CCIE 7/10, 3/3, 0/5, 0/1, and 0/4, while the co-agglutination was 6/10, 3/3, 0/5, 0/1, and 0/4. The standard phenol/ chloroform extraction was used to purify DNA from the sputum’s pellet, followed by precipitation of ethanol. Each reaction set involved sterile water in place of DNA as a negative control. There is a change in the genetic sequence of drug-resistant isolates, CTT to CTA and TTT to TAT.


Author(s):  
Benjamin Onyema Eledo ◽  
Edidiong Tommy ◽  
Emmanuel Onuoha ◽  
Kingsley Dunga ◽  
Okezie Okamgba

This study determined the prevalence of Rheumatoid arthritis in elderly people that attended pilgrimage center, Elele Rivers state, Nigeria. A total of 200 subjects attending the pilgrimage center were examined for Rhematoid Factor using Latex agglutination method. The data was subjected to statistical analysis using Statistical Package for Social Sciences (SPSS) version 16.0. A prevalence of 4% was seen in the female subjects within the age bracket of 65-80 years and a prevalence of 1% was seen in the male subjects within the age bracket of 61-70 years. It showed that Rheumatoid arthritis was positive in 5% of the population and negative in 95% of the population, which was statistically significant (p< 0.05). In conclusion, there is a significant increase in the overall prevalence of rheumatoid arthritis in elderly subjects that attend pilgrimage center, Elele. Therefore proper care should be instituted to  avoid unnecessary complications associated with this ailment.


2020 ◽  
Vol 59 (3) ◽  
pp. 102732
Author(s):  
Vivek Krishnan ◽  
Veena Shenoy ◽  
Shilna Sunny ◽  
Ramanathan Thambiran ◽  
Dhanya Keezhattoor ◽  
...  

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