Design, Synthesis, and Functional Evaluation of CO-Releasing Molecules Triggered by Penicillin G Amidase as a Model Protease

2015 ◽  
Vol 54 (42) ◽  
pp. 12314-12318 ◽  
Author(s):  
Nikolay S. Sitnikov ◽  
Yingchun Li ◽  
Danfeng Zhang ◽  
Benito Yard ◽  
Hans-Günther Schmalz
Keyword(s):  
Penicillin G ◽  
Functional Evaluation ◽  
Design Synthesis

scholarly journals Design, Synthesis and Evaluation of Enzyme-Responsive Fluorogenic Probes Based on Pyridine-Flanked Diketopyrrolopyrrole Dyes

2020 ◽  
Author(s):  
Sébastien Jenni ◽  
Flavien Ponsot ◽  
Pierre Baroux ◽  
Lucille Collard ◽  
Takayuki Ikeno ◽  
...  
Keyword(s):  
Charge State ◽  
Systematic Study ◽  
Fluorescent Probes ◽  
Penicillin G ◽  
Redox Enzymes ◽  
Design Synthesis ◽  
Rapid Construction ◽  
Fluorogenic Dyes ◽  
Fluorogenic Probes ◽  
Scope Of Application

The ever-growing demand for fluorogenic dyes usable in the rapid construction of analyte-responsive fluorescent probes, has recently contributed to a revival of interest in the chemistry of diketopyrrolopyrrole (DPP) pigments. In this context, we have explored the potential of symmetrical and unsymmetrical DPP derivatives bearing two or one 4-pyridyl substituents acting as optically tunable group(s). The unique fluorogenic behavior of these molecules, closely linked to N-substitution/charge state of their pyridine unit (i.e., neutral pyridine or cationic pyridinium), has been used to design DPP-based fluorescent probes for detection of hypoxia-related redox enzymes and penicillin G acylase (PGA). In this paper, we describe synthesis, spectral characterization and bioanalytical validations of these probes. Dramatic differences in terms of aqueous stability and enzymatic fluorescence activation were observed. This systematic study enables to delineate the scope of application of pyridine-flanked DPP fluorophores in the field of enzyme biosensing.

Design, Synthesis, and Functional Evaluation of Leukocyte Function Associated Antigen-1 Antagonists in Early and Late Stages of Cancer Development

2013 ◽  
Vol 56 (3) ◽  
pp. 735-747 ◽  
Author(s):  
Eider San Sebastián ◽  
Tahl Zimmerman ◽  
Aizpea Zubia ◽  
Yosu Vara ◽  
Elyette Martin ◽  
...  
Keyword(s):  
Cancer Development ◽  
Functional Evaluation ◽  
Design Synthesis ◽  
Leukocyte Function ◽  
Late Stages

scholarly journals Design, Synthesis and Evaluation of Enzyme-Responsive Fluorogenic Probes Based on Pyridine-Flanked Diketopyrrolopyrrole Dyes

2020 ◽  
Author(s):  
Sébastien Jenni ◽  
Flavien Ponsot ◽  
Pierre Baroux ◽  
Lucille Collard ◽  
Takayuki Ikeno ◽  
...  
Keyword(s):  
Charge State ◽  
Systematic Study ◽  
Fluorescent Probes ◽  
Penicillin G ◽  
Redox Enzymes ◽  
Design Synthesis ◽  
Rapid Construction ◽  
Fluorogenic Dyes ◽  
Fluorogenic Probes ◽  
Scope Of Application

The ever-growing demand for fluorogenic dyes usable in the rapid construction of analyte-responsive fluorescent probes, has recently contributed to a revival of interest in the chemistry of diketopyrrolopyrrole (DPP) pigments. In this context, we have explored the potential of symmetrical and unsymmetrical DPP derivatives bearing two or one 4-pyridyl substituents acting as optically tunable group(s). The unique fluorogenic behavior of these molecules, closely linked to N-substitution/charge state of their pyridine unit (i.e., neutral pyridine or cationic pyridinium), has been used to design DPP-based fluorescent probes for detection of hypoxia-related redox enzymes and penicillin G acylase (PGA). In this paper, we describe synthesis, spectral characterization and bioanalytical validations of these probes. Dramatic differences in terms of aqueous stability and enzymatic fluorescence activation were observed. This systematic study enables to delineate the scope of application of pyridine-flanked DPP fluorophores in the field of enzyme biosensing.

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

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