scholarly journals Improved intact peptide and protein quantitation by LC‐MS: Battling the deleterious effects of analyte adsorption

2020 ◽  
Author(s):  
EmmaRae L. Murphy ◽  
Andrew P. Joy ◽  
Rodney J. Ouellette ◽  
David A. Barnett
Keyword(s):  
Protein Quantitation

Protein Quantitation Using Isotope-Assisted Mass Spectrometry

2010 ◽  
Vol 39 (1) ◽  
pp. 291-308 ◽  
Author(s):  
Kelli G. Kline ◽  
Michael R. Sussman
Keyword(s):  
Mass Spectrometry ◽  
Protein Quantitation

Automated Chloroform-Methanol Protein Extraction on the Biomek-FX Liquid Handler System v2

2022 ◽  
Author(s):  
Yan Chen ◽  
Tad Ogorzalek ◽  
Nurgul Kaplan Lease ◽  
Jennifer Gin ◽  
Christopher J Petzold
Keyword(s):  
Sample Preparation ◽  
Protein Extraction ◽  
Tryptic Digestion ◽  
Gram Negative ◽  
Protein Quantitation ◽  
System P ◽  
Automated Protocol ◽  
Chloroform Methanol ◽  
Extract Protein

This protocol details steps to extract protein from Gram-negative bacterial or fungal cells (that have been pretreated with zymolyase) in quantitative proteomic workflows by using a Biomek FX liquid handler system. It is a semi-automated protocol that includes several 'pause' steps for centrifugation steps that are conducted manually "off-deck". This protocol works best as part of an automated proteomic sample preparation workflow with: Automated Protein Quantitation with the Biomek-FX liquid handler system and Automated Protein Normalization and Tryptic Digestion on a Biomek-NX Liquid Handler System

Basic Concepts in Mass Spectrometry and Protein Quantitation

2016 ◽  
pp. 15-32
Author(s):  
Jake Cosme ◽  
Irina Tchernyshyov ◽  
Anthony Gramolini
Keyword(s):  
Mass Spectrometry ◽  
Protein Quantitation ◽  
Basic Concepts

scholarly journals An Evalution of Measurement of Cell Growth in Tissue Culture with Protein Quantitation

1992 ◽  
Vol 46 (2) ◽  
pp. 398-403
Author(s):  
Sen Higashi ◽  
Tomoko Ohsumi ◽  
Kayoko Kuroki
Keyword(s):  
Tissue Culture ◽  
Cell Growth ◽  
Protein Quantitation
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