Spectrofluorimetric determination of atenolol from human urine using high-affinity molecularly imprinted solid-phase extraction sorbent

Luminescence ◽  
2017 ◽  
Vol 32 (8) ◽  
pp. 1391-1397 ◽  
Author(s):  
Yagoup Gorbani ◽  
Hüma Yılmaz ◽  
Hasan Basan
Keyword(s):  
Solid Phase Extraction ◽  
Human Urine ◽  
Solid Phase ◽  
Phase Extraction ◽  
Molecularly Imprinted ◽  
High Affinity ◽  
Spectrofluorimetric Determination

Solid-phase extraction using molecularly imprinted polymer for determination of ochratoxin A in human urine

2015 ◽  
Vol 8 (1) ◽  
pp. 37-43 ◽  
Author(s):  
L. Xie ◽  
P. Sheng ◽  
W. Kong ◽  
X. Zhao ◽  
Z. Ou-Yang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Ochratoxin A ◽  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Solid Phase ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

A rapid, selective and reliable sample preparation technique employing solid-phase extraction (SPE) based on molecularly imprinted polymer (MIP) for the determination of ochratoxin A (OTA) in human urine was described. After sample adjustment to pH 2.5 with 0.1 M HCl, the urine sample was loaded onto the MIP-SPE column, and after a wash step, OTA was eluted for measurement by ultra-high performance liquid chromatography coupled with fluorescence detection. Key parameters which affected the MIP-SPE extraction efficiency were optimized as was the detection method. Under the optimised conditions, the limits of detection and quantification for OTA in urine were 0.2 ng/ml and 0.6 ng/ml, respectively. The recoveries for OTA in urine, spiked at the 0.6, 6.0 and 60 ng/ml levels, ranged from 92.0 to 98.9%. Sixty urine samples were analysed, of which four were found to contain OTA at concentrations ranging from 0.022 to 0.083 ng/ml; the positive results were confirmed by liquid chromatography coupled with tandem mass spectrometry. OTA determination in urine is a good indicator for human exposure to the mycotoxin, and this is the first report on OTA contamination in Chinese people.

Molecularly imprinted pipette-tip solid phase extraction for selective determination of fluoroquinolones in human urine using HPLC-DAD

2016 ◽  
Vol 1033-1034 ◽  
pp. 27-39 ◽  
Author(s):  
Hanna Leijoto de Oliveira ◽  
Sara da Silva Anacleto ◽  
Anny Talita Maria da Silva ◽  
Arnaldo César Pereira ◽  
Warley de Souza Borges ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Human Urine ◽  
Solid Phase ◽  
Phase Extraction ◽  
Molecularly Imprinted ◽  
Selective Determination ◽  
Pipette Tip

scholarly journals Assessment of the Performance of Solid Phase Extraction Based on Pipette Tip Employing a Hybrid Molecularly Imprinted Polymer as an Adsorbent for Enantioselective Determination of Albendazole Sulfoxide

2019 ◽  
Vol 57 (7) ◽  
pp. 671-678 ◽  
Author(s):  
Sara da Silva Anacleto ◽  
Hanna Leijoto de Oliveira ◽  
Anny Talita Maria da Silva ◽  
Tienne Aparecida do Nascimento ◽  
Marcella Matos Cordeiro Borges ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Solid Phase ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Albendazole Sulfoxide ◽  
Pipette Tip

Abstract Herein, an organic–inorganic hybrid molecularly imprinted polymer (MIP) was successfully synthesized with albendazole sulfoxide (ABZSO) as a template and 3-(trimethoxysilyl)propyl methacrylate, a bifunctional group compound, as a single cross-linking agent. In this study, a simple method using HPLC–DAD was developed for the determination of ABZSO enantiomers in human urine using pipette tip-based molecularly imprinted polymer solid phase extraction (PT–MIP–SPE). Enantioseparation with satisfactory retention times (5.17 and 7.09 min), acceptable theoretical plates (N = 4,535 and 5,091) and strong resolution (Rs = 5.45) was performed with an Agilent® Eclipse Plus C18 (100 mm × 4.6 mm, 3.5 μm) coupled with a Chiralpak® IA column (100 mm × 4.6 mm, 3 μm), a mixture with ethanol:water (50:50, v/v) as the mobile phase, temperature at 40°C, flow rate at 0.9 mL min−1 and λ = 230 nm. Thereafter, certain parameters affecting the PT–MIP–SPE were investigated in detail and the better conditions were: 300 μL of water as washing solvent, 500 μL of ethanol:acetic acid (9:1, v/v) as eluting solvent, 20 mg of MIP, 500 μL of human urine at pH 9 and no addition of NaCl. Recoveries/relative standard deviation (RSD%) for (R)-(+)-ABZSO and (S)-(−)-ABZSO were 78.2 ± 0.2% and 69.7 ± 1.7%, respectively.

Efficient strategy for the selective determination of dopamine in human urine by molecularly imprinted solid-phase extraction

2016 ◽  
Vol 39 (5) ◽  
pp. 895-903 ◽  
Author(s):  
Piotr Luliński ◽  
Magdalena Bamburowicz-Klimkowska ◽  
Mariusz Dana ◽  
Mirosław Szutowski ◽  
Dorota Maciejewska
Keyword(s):  
Solid Phase Extraction ◽  
Human Urine ◽  
Solid Phase ◽  
Phase Extraction ◽  
Molecularly Imprinted ◽  
Selective Determination ◽  
Efficient Strategy
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