Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency

2003 ◽  
Vol 84 (3) ◽  
pp. 332-342 ◽  
Author(s):  
Phuong Lan Pham ◽  
Sylvie Perret ◽  
Huyen Chau Doan ◽  
Brian Cass ◽  
Gilles St-Laurent ◽  
...  
Keyword(s):  
Cell Growth ◽  
Large Scale ◽  
Transfection Efficiency ◽  
Transient Transfection ◽  
Serum Free ◽  
Serum Free Suspension

Serum-Free Suspension Large-Scale Transient Transfection of CHO Cells in WAVE Bioreactors

2006 ◽  
Vol 34 (2) ◽  
pp. 191-200 ◽  
Author(s):  
Raj Haldankar ◽  
Danqing Li ◽  
Zane Saremi ◽  
Claudia Baikalov ◽  
Rohini Deshpande
Keyword(s):  
Cho Cells ◽  
Large Scale ◽  
Transient Transfection ◽  
Serum Free ◽  
Serum Free Suspension

Serum-free large-scale transient transfection of CHO cells

2004 ◽  
Vol 87 (4) ◽  
pp. 537-545 ◽  
Author(s):  
Madiha Derouazi ◽  
Philippe Girard ◽  
Fr�d�ric Van Tilborgh ◽  
Keyvan Iglesias ◽  
Natalie Muller ◽  
...  
Keyword(s):  
Cho Cells ◽  
Large Scale ◽  
Transient Transfection ◽  
Serum Free

scholarly journals Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII

2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Kamilla Swiech ◽  
Amine Kamen ◽  
Sven Ansorge ◽  
Yves Durocher ◽  
Virgínia Picanço-Castro ◽  
...  
Keyword(s):  
Cell Culture ◽  
Transient Transfection ◽  
Efficient Production ◽  
Hek 293 ◽  
Serum Free ◽  
Serum Free Suspension

scholarly journals Inducible Packaging Cells for Large-scale Production of Lentiviral Vectors in Serum-free Suspension Culture

2008 ◽  
Vol 16 (3) ◽  
pp. 500-507 ◽  
Author(s):  
Sophie Broussau ◽  
Nadine Jabbour ◽  
Guillaume Lachapelle ◽  
Yves Durocher ◽  
Rosanne Tom ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Large Scale ◽  
Lentiviral Vectors ◽  
Scale Production ◽  
Serum Free ◽  
Large Scale Production ◽  
Serum Free Suspension

scholarly journals 292. Towards Large-Scale Manufacturing of Adeno-Associated Virus by Transient Transfection of HEK293 Suspension Cells in a Stirred Tank Bioreactor Using Serum-Free Medium

2016 ◽  
Vol 24 ◽  
pp. S117-S118 ◽  
Author(s):  
Parminder Chahal ◽  
Érica Alessandra Schulze ◽  
Alice Bernier ◽  
Stephane Lanthier ◽  
Nathalie Coulombe ◽  
...  
Keyword(s):  
Large Scale ◽  
Transient Transfection ◽  
Stirred Tank ◽  
Serum Free Medium ◽  
Free Medium ◽  
Suspension Cells ◽  
Stirred Tank Bioreactor ◽  
Adeno Associated Virus ◽  
Serum Free

Stepwise Development of Biomimetic Chimeric Peptides for Gene Delivery

2020 ◽  
Vol 27 (8) ◽  
pp. 698-710
Author(s):  
Roya Cheraghi ◽  
Mahboobeh Nazari ◽  
Mohsen Alipour ◽  
Saman Hosseinkhani
Keyword(s):  
Gene Delivery ◽  
Targeted Delivery ◽  
Viral Vectors ◽  
Large Scale ◽  
Transfection Efficiency ◽  
Cationic Lipids ◽  
Target Cells ◽  
Scale Production ◽  
Stepwise Development ◽  
Chimeric Peptides

Gene-based therapy largely relies on the vector type that allows a selective and efficient transfection into the target cells with maximum efficacy and minimal toxicity. Although, genes delivered utilizing modified viruses transfect efficiently and precisely, these vectors can cause severe immunological responses and are potentially carcinogenic. A promising method of overcoming this limitation is the use of non-viral vectors, including cationic lipids, polymers, dendrimers, and peptides, which offer potential routes for compacting DNA for targeted delivery. Although non-viral vectors exhibit reduced transfection efficiency compared to their viral counterpart, their superior biocompatibility, non-immunogenicity and potential for large-scale production make them increasingly attractive for modern therapy. There has been a great deal of interest in the development of biomimetic chimeric peptides. Biomimetic chimeric peptides contain different motifs for gene translocation into the nucleus of the desired cells. They have motifs for gene targeting into the desired cell, condense DNA into nanosize particles, translocate the gene into the nucleus and enhance the release of the particle into the cytoplasm. These carriers were developed in recent years. This review highlights the stepwise development of the biomimetic chimeric peptides currently being used in gene delivery.

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