scholarly journals Method for determining oxygen consumption rates of static cultures from microplate measurements of pericellular dissolved oxygen concentration

2005 ◽  
Vol 91 (3) ◽  
pp. 392-392 ◽  
Author(s):  
Richard D. Guarino ◽  
Laura E. Dike ◽  
Tariq A. Haq ◽  
Jon A. Rowley ◽  
J. Bruce Pitner ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Dissolved Oxygen ◽  
Oxygen Concentration ◽  
Dissolved Oxygen Concentration ◽  
Consumption Rates

Method for determining oxygen consumption rates of static cultures from microplate measurements of pericellular dissolved oxygen concentration

2004 ◽  
Vol 86 (7) ◽  
pp. 775-787 ◽  
Author(s):  
Richard D. Guarino ◽  
Laura E. Dike ◽  
Tariq A. Haq ◽  
Jon A. Rowley ◽  
J. Bruce Pitner ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Dissolved Oxygen ◽  
Oxygen Concentration ◽  
Dissolved Oxygen Concentration ◽  
Consumption Rates

Positioning changes of mayfly nymphs due to behavioral regulation of oxygen consumption

1980 ◽  
Vol 58 (4) ◽  
pp. 618-622 ◽  
Author(s):  
Michael J. Wiley ◽  
Steven L. Kohler
Keyword(s):  
Oxygen Consumption ◽  
Dissolved Oxygen ◽  
Oxygen Concentration ◽  
Field Conditions ◽  
Experimental Investigations ◽  
Behavioral Patterns ◽  
Behavioral Regulation ◽  
Dissolved Oxygen Concentration ◽  
Habitat Distribution ◽  
Renewal Rate

Experimental investigations in a small artificial stream showed that the positioning of mayfly nymphs (Ephemeroptera) on stones varied with dissolved oxygen concentration (DO). At low DO levels nymphs moved to current-exposed positions, presumably to increase the renewal rate of oxygen at respiratory exchange surfaces. The expected magnitude of positioning changes under field conditions was determined and suggests that behavioral regulation of oxygen consumption may commonly influence both habitat distribution and diel behavioral patterns. The implications of these results to drift studies are also discussed.

Some Factors Affecting the Oxygen Consumption of Asellus

1960 ◽  
Vol 37 (4) ◽  
pp. 706-718
Author(s):  
R. W. EDWARDS ◽  
M. A. LEARNER
Keyword(s):  
Oxygen Consumption ◽  
Dissolved Oxygen ◽  
Constant Volume ◽  
Asellus Aquaticus ◽  
Dissolved Oxygen Concentration ◽  
Factors Affecting ◽  
Consumption Rates ◽  
Rate Of Oxygen Consumption ◽  
Wet Weight ◽  
Males And Females

1. The oxygen-consumption rates of Asellus aquaticus (males and females) have been measured at 10 and 20° C. using a constant-volume respirometer, and the effect of starvation for 24 hr. investigated. The oxygen consumption is approximately proportional to the 0.7 power of the wet weight. The rate of oxygen consumption at 20° C. is greater than at 10° C. by a factor of 1.5. 2. The oxygen-consumption rates of A. aquaticus and A. meridianus have been measured at 20° C. in a flowing-water respirometer employing a polarographic technique for the measurement of dissolved-oxygen concentrations. The oxygen consumptions of A. aquaticus and A. meridianus are similar and decrease by 15-20% when the dissolved-oxygen concentration falls from 8.3 to 1.5 p.p.m. 3. The oxygen consumption of A. aquaticus is between 35 and 75% higher in the polarographic respirometer than in the constant-volume respirometer.

scholarly journals Rapid Method for Assessing Oxygen Consumption Rate of Cells from Transient-state Measurements of Pericellular Dissolved Oxygen Concentration

2005 ◽  
Vol 49 (2-3) ◽  
pp. 133-141 ◽  
Author(s):  
Laura E. Dike ◽  
Haiyan Xia ◽  
Richard D. Guarino ◽  
Sharon C. Presnell ◽  
Mark R. Timmins
Keyword(s):  
Oxygen Consumption ◽  
Dissolved Oxygen ◽  
Rapid Method ◽  
Oxygen Concentration ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
Transient State ◽  
Dissolved Oxygen Concentration

scholarly journals Hyperosmotic Agents and Antibiotics Affect Dissolved Oxygen and pH Concentration Gradients in Staphylococcus aureus Biofilms

2017 ◽  
Vol 83 (6) ◽  
Author(s):  
Mia Mae Kiamco ◽  
Erhan Atci ◽  
Abdelrhman Mohamed ◽  
Douglas R. Call ◽  
Haluk Beyenal
Keyword(s):  
Staphylococcus Aureus ◽  
Dissolved Oxygen ◽  
Oxygen Concentration ◽  
Time Frame ◽  
Bactericidal Effect ◽  
Dissolved Oxygen Concentration ◽  
Content Type ◽  
Consumption Rates ◽  
Wound Biofilms ◽  
Medical Grade

ABSTRACTBiofilms on wound surfaces are treated topically with hyperosmotic agents, such as medical-grade honey and cadexomer iodine; in some cases, these treatments are combined with antibiotics. Tissue repair requires oxygen, and a low pH is conducive to oxygen release from red blood cells and epithelialization. We investigated the variation of dissolved oxygen concentration and pH with biofilm depth and the variation in oxygen consumption rates when biofilms are challenged with medical-grade honey or cadexomer iodine combined with vancomycin or ciprofloxacin. Dissolved oxygen and pH depth profiles inStaphylococcus aureusbiofilms were measured using microelectrodes. The presence of cadexomer iodine with vancomycin or ciprofloxacin on the surface of the biofilm permitted a measurable concentration of oxygen at greater biofilm depths (101.6 ± 27.3 μm,P= 0.02; and 155.5 ± 27.9 μm,P= 0.016, respectively) than in untreated controls (30.1 μm). Decreases in pH of ∼0.6 and ∼0.4 units were observed in biofilms challenged with medical-grade honey alone and combined with ciprofloxacin, respectively (P< 0.001 and 0.01, respectively); the number of bacteria recovered from biofilms was significantly reduced (1.26 log) by treatment with cadexomer iodine and ciprofloxacin (P= 0.002) compared to the untreated control. Combining cadexomer iodine and ciprofloxacin improved dissolved oxygen concentration and penetration depth into the biofilm, while medical-grade honey was associated with a lower pH; not all treatments established a bactericidal effect in the time frame used in the experiments.IMPORTANCEReports about using hyperosmotic agents and antibiotics against wound biofilms focus mostly on killing bacteria, but the results of these treatments should additionally be considered in the context of how they affect physiologically important parameters, such as oxygen concentration and pH. We confirmed that the combination of a hyperosmotic agent and an antibiotic results in greater dissolved oxygen and reduced pH within anS. aureusbiofilm.

The Effects of Dissolved Oxygen Concentration, Fractional Oxygen Consumption, and Additives on JP-8 Thermal Stability

1997 ◽  
Vol 119 (4) ◽  
pp. 822-829 ◽  
Author(s):  
J. S. Ervin ◽  
T. F. Williams ◽  
S. P. Heneghan ◽  
S. Zabarnick
Keyword(s):  
Thermal Stability ◽  
Oxygen Consumption ◽  
Heat Exchanger ◽  
Dissolved Oxygen ◽  
Oxygen Concentration ◽  
Jet Fuel ◽  
Combined Effects ◽  
Fuel System ◽  
Dissolved Oxygen Concentration ◽  
Additive Package

Since dissolved oxygen participates in fuel deposit formation, knowledge of the effects of dissolved oxygen concentration on fuel thermal stability is critical for fuel system design. In this work, the combined effects of dissolved oxygen availability and additives on jet fuel thermal stability are studied. Experiments with JP-8 jet fuel were conducted in a three-part heat exchanger that simulated a complex thermal and flow environment. The dissolved oxygen content at the flow inlet was varied, and deposition was studied under conditions of either fractional or complete oxygen consumption. The effects of a thermal stability additive package were also studied. An intriguing result found with JP-8 fuels is an increase in deposits formed in heated regions for decreased oxygen consumption, but inverse behavior with the additive package.

scholarly journals Oxygen consumption of Litopenaeus vannamei juveniles in heterotrophic medium with zero water exchange

2009 ◽  
Vol 44 (5) ◽  
pp. 534-538 ◽  
Author(s):  
Luis Vinatea ◽  
Alfredo Olivera Gálvez ◽  
Jesús Venero ◽  
John Leffler ◽  
Craig Browdy
Keyword(s):  
Oxygen Consumption ◽  
Dissolved Oxygen ◽  
Oxygen Concentration ◽  
Litopenaeus Vannamei ◽  
Water Exchange ◽  
Consumption Rate ◽  
Light Conditions ◽  
Dissolved Oxygen Concentration ◽  
Dark Conditions ◽  
Over Time

This work aimed at determining the dissolved oxygen consumption rate of Litopenaeus vannamei juveniles maintained in a microbial biofloc raceway system at high density with no aeration. Three 4 L bottles were filled for each treatment, sealed hermetically, and placed in an enclosed greenhouse raceway system. Four shrimp (13.2±1.42 g) were assigned to two sets of the bottles, which underwent the following treatments: light conditions with no shrimp; dark conditions with no shrimp; light conditions with shrimp; and dark conditions with shrimp. Dissolved oxygen content was measured every 10 min for 30 min. A quadratic behavior was observed in dissolved oxygen concentration over time. Significant differences for oxigen consumption were observed only at 10 and 20 min between shrimp maintained in the dark and those under light conditions. At 10 min, a higher value was observed in shrimp maintained under light, and at 20 min, in the dark. Significant differences between 10 and 20 min and between 10 and 30 min were observed when oxygen consumption was analyzed over time in the presence of light. Under dark conditions there were significant differences only between 20 and 30 min. Lethal oxygen concentration (0.65 mg L-1) would be reached in less than one hour either under light or dark conditions with no aeration.

scholarly journals The Effects of Dissolved Oxygen Concentration, Fractional Oxygen Consumption, and Additives on JP-8 Thermal Stability

1996 ◽  
Author(s):  
J. S. Ervin ◽  
T. F. Williams ◽  
S. P. Heneghan ◽  
S. Zabarnick
Keyword(s):  
Thermal Stability ◽  
Oxygen Consumption ◽  
Heat Exchanger ◽  
Dissolved Oxygen ◽  
Oxygen Concentration ◽  
Jet Fuel ◽  
Combined Effects ◽  
Fuel System ◽  
Dissolved Oxygen Concentration ◽  
Additive Package

Since dissolved oxygen participates in fuel deposit formation, knowledge of the effects of dissolved oxygen concentration on fuel thermal stability is critical for fuel system design. In this work, the combined effects of dissolved oxygen availability and additives on jet fuel thermal stability are studied. Experiments with JP-8 jet fuel were conducted in a three-part heat exchanger which simulated a complex thermal and flow environment. The dissolved oxygen content at the flow inlet was varied, and deposition was studied under conditions of either fractional or complete oxygen consumption. The effects of a thermal stability additive package were also studied. An intriguing result found with JP-8 fuels is an increase in deposits formed in heated regions for decreased oxygen consumption, but inverse behavior with the additive package.

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