Understanding the intracellular effect of enhanced nutrient feeding toward high titer antibody production process

2011 ◽  
Vol 108 (5) ◽  
pp. 1078-1088 ◽  
Author(s):  
Marcella Yu ◽  
Zhilan Hu ◽  
Efren Pacis ◽  
Natarajan Vijayasankaran ◽  
Amy Shen ◽  
...  
Keyword(s):  
Production Process ◽  
Antibody Production ◽  
High Titer ◽  
Intracellular Effect ◽  
Nutrient Feeding

Model-Based Monitoring and Control of a Monoclonal Antibody Production Process

1995 ◽  
pp. 665-670
Author(s):  
R. K. Biener ◽  
W. Waldraff ◽  
E. D. Gilles ◽  
J. Haas ◽  
W. NoÉ ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Production Process ◽  
Antibody Production ◽  
Monitoring And Control ◽  
Monoclonal Antibody Production ◽  
Model Based ◽  
And Control

scholarly journals Interleukin 6 Dependence of Anti-DNA Antibody Production: Evidence for Two Pathways of Autoantibody Formation in Pristane-induced Lupus

1998 ◽  
Vol 188 (5) ◽  
pp. 985-990 ◽  
Author(s):  
Hanno B. Richards ◽  
Minoru Satoh ◽  
Melody Shaw ◽  
Claude Libert ◽  
Valeria Poli ◽  
...  
Keyword(s):  
Disease Activity ◽  
Antibody Production ◽  
High Titer ◽  
Enzyme Linked Immunosorbent Assay ◽  
Autoantibody Production ◽  
Crithidia Luciliae ◽  
Dsdna Antibody ◽  
Dna Antibodies ◽  
Antibody Levels ◽  
Phosphate Buffered Saline

Pristane induces a lupus-like syndrome in nonautoimmune mice characterized by the development of glomerulonephritis and lupus-associated autoantibodies. This is accompanied by overproduction of interleukin (IL)-6, a cytokine linked with autoimmune phenomena. The goal of this study was to evaluate the role of IL-6 in autoantibody production in pristane-induced lupus. BALB/cAn IL-6–deficient (−/−) and –intact (+/+) mice were treated with pristane or phosphate-buffered saline, and autoantibody production was evaluated. Pristane induced high levels of immunoglobulin (Ig)G anti-single-stranded DNA, –double-stranded (ds)DNA, and -chromatin antibodies in IL-6+/+, but not IL-6−/− mice by enzyme-linked immunosorbent assay. High titer IgG anti-dsDNA antibodies also were detected in sera from +/+, but not −/−, mice by Crithidia luciliae kinetoplast staining. The onset of IgG anti-dsDNA antibody production in +/+ mice occurred >5 mo after pristane treatment, well after the onset of nephritis, suggesting that these antibodies are not directly responsible for inducing renal disease. In contrast to anti-DNA, the frequencies of anti-nRNP/Sm and anti-Su antibodies were similar in pristane-treated IL-6−/− and IL-6+/+ mice. However, levels were higher in the +/+ group. These results suggest that IgG anti-DNA and chromatin antibodies in pristane-treated mice are strictly IL-6 dependent, whereas induction of anti-nRNP/Sm and Su autoantibodies is IL-6 independent. The IL-6 dependence of anti-DNA, but not anti-nRNP/Sm, may have implications for understanding the patterns of autoantibody production in lupus. Anti-DNA antibodies are produced transiently, mainly during periods of disease activity, whereas anti-nRNP/Sm antibody levels are relatively insensitive to disease activity. This may reflect the differential IL-6 dependence of the two responses.

scholarly journals Scale-up considerations for monoclonal antibody production process: an oxygen transfer flux approach

2013 ◽  
Vol 7 (S6) ◽  
Author(s):  
Laura Gimenez ◽  
Claire Simonet ◽  
Laetitia Malphettes
Keyword(s):  
Monoclonal Antibody ◽  
Production Process ◽  
Antibody Production ◽  
Oxygen Transfer ◽  
Scale Up ◽  
Monoclonal Antibody Production

scholarly journals THE DEVELOPMENT OF THE IMMUNE RESPONSE

1963 ◽  
Vol 117 (3) ◽  
pp. 479-496 ◽  
Author(s):  
Joseph A. Bellanti ◽  
Donald V. Eitzman ◽  
John B. Robbins ◽  
Richard T. Smith
Keyword(s):  
Molecular Weight ◽  
Immune Response ◽  
Antibody Production ◽  
High Titer ◽  
Lower Molecular Weight ◽  
Adult Rabbit ◽  
Newborn Animal ◽  
Flagellar Antigen ◽  
Type Response

By intensive stimulation with large amounts of Salmonella flagellar antigen, newborn rabbits were induced to form high titer flagellar agglutinins usually by the 7th to 10th day of life. Characterization of the agglutinins at various times during the first 30 days of life revealed that the earliest antibody which appeared was a gamma-1 macroglobulin, and that 7S gamma-2 globulins did not appear until the 4th or 5th week of life. In contrast, the adult animals produced macroglobulin antibodies for only 3 to 5 days before the lower molecular weight variety appeared. The infant macroglobulin appears to be similar in all respects to adult macroglobulin antibodies. These data are interpreted to indicate that the newborn and adult rabbit differ in their response to this type of stimulus not in timing of macroglobulin antibody production, but chiefly in the prolonged interval, which precedes the development of the capacity for the 7S type response in the newborn animal.

Case Study: An accelerated 8-day monoclonal antibody production process based on high seeding densities

2013 ◽  
Vol 29 (3) ◽  
pp. 829-832 ◽  
Author(s):  
Ishai Padawer ◽  
Wai Lam W. Ling ◽  
Yunling Bai
Keyword(s):  
Monoclonal Antibody ◽  
Production Process ◽  
Antibody Production ◽  
Monoclonal Antibody Production

High titer oncolytic measles virus production process by integration of dielectric spectroscopy as online monitoring system

2018 ◽  
Vol 115 (5) ◽  
pp. 1186-1194 ◽  
Author(s):  
Tanja A. Grein ◽  
Daniel Loewe ◽  
Hauke Dieken ◽  
Denise Salzig ◽  
Tobias Weidner ◽  
...  
Keyword(s):  
Production Process ◽  
Monitoring System ◽  
Dielectric Spectroscopy ◽  
Measles Virus ◽  
Online Monitoring ◽  
High Titer ◽  
Virus Production ◽  
Online Monitoring System

scholarly journals Suppression of IgE antibody production in SJL mice. III. Characterization of a suppressor substance extracted from normal SJL spleen cells.

1977 ◽  
Vol 145 (6) ◽  
pp. 1501-1510 ◽  
Author(s):  
N Watanabe ◽  
Z Ovary
Keyword(s):  
Antibody Production ◽  
High Titer ◽  
Gel Filtration ◽  
Keyhole Limpet Hemocyanin ◽  
Cell Extract ◽  
Nippostrongylus Brasiliensis ◽  
Spleen Cells ◽  
Ige Antibody ◽  
Third Stage

SJL mice were immunized with 1 microng dinitrophenylated keyhole limpet hemocyanin in 1 mg Al(OH)3. The mice were infected 21 days later with 750 third stage larvae of Nippostrongylus brasiliensis. On day 35, 14 days after infection, they were injected with 1 microng DNP-N, brasiliensis extract (Nb) in 1 mg Al(OH)3. In order to obtain high titer and persistent anti-DNP IgE antibody the mice were irradiated (540 R) 1 day after injection of DNP-Nb. Suppression of anti-DNP IgE antibody production was induced by spleen cells from normal SJL mice. Suppression of IgE antibody response is also obtained by an extract from normal SJL spleen cells. The suppressor substance from normal SJL spleen cell extract is a heat-labile protein, and is not absorbed by anti-mouse immunoglobulin. The mol wt of this substance is larger than 300,000 daltons as determined by gel filtration on Sephadex G-200, but after ultracentifugation, the supernate still has suppressive activity on IgE antibody production.

Design and operation of a continuous integrated monoclonal antibody production process

2017 ◽  
Vol 33 (5) ◽  
pp. 1303-1313 ◽  
Author(s):  
Fabian Steinebach ◽  
Nicole Ulmer ◽  
Moritz Wolf ◽  
Lara Decker ◽  
Veronika Schneider ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Production Process ◽  
Antibody Production ◽  
Monoclonal Antibody Production

Resilient immortals, characterizing and utilizing Bax/Bak deficient Chinese hamster ovary (CHO) cells for high titer antibody production

2013 ◽  
Vol 29 (3) ◽  
pp. 727-737 ◽  
Author(s):  
Shahram Misaghi ◽  
Yan Qu ◽  
Andrew Snowden ◽  
Jennifer Chang ◽  
Brad Snedecor
Keyword(s):  
Antibody Production ◽  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
High Titer ◽  
Chinese Hamster
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