scholarly journals THE DEVELOPMENT OF THE IMMUNE RESPONSE

1963 ◽  
Vol 117 (3) ◽  
pp. 479-496 ◽  
Author(s):  
Joseph A. Bellanti ◽  
Donald V. Eitzman ◽  
John B. Robbins ◽  
Richard T. Smith
Keyword(s):  
Molecular Weight ◽  
Immune Response ◽  
Antibody Production ◽  
High Titer ◽  
Lower Molecular Weight ◽  
Adult Rabbit ◽  
Newborn Animal ◽  
Flagellar Antigen ◽  
Type Response

By intensive stimulation with large amounts of Salmonella flagellar antigen, newborn rabbits were induced to form high titer flagellar agglutinins usually by the 7th to 10th day of life. Characterization of the agglutinins at various times during the first 30 days of life revealed that the earliest antibody which appeared was a gamma-1 macroglobulin, and that 7S gamma-2 globulins did not appear until the 4th or 5th week of life. In contrast, the adult animals produced macroglobulin antibodies for only 3 to 5 days before the lower molecular weight variety appeared. The infant macroglobulin appears to be similar in all respects to adult macroglobulin antibodies. These data are interpreted to indicate that the newborn and adult rabbit differ in their response to this type of stimulus not in timing of macroglobulin antibody production, but chiefly in the prolonged interval, which precedes the development of the capacity for the 7S type response in the newborn animal.

scholarly journals Characterization of an unusual isoenzyme of N-acetyl-β-d-hexosaminidase from a human colonic carcinoma cell line

1981 ◽  
Vol 193 (1) ◽  
pp. 109-113 ◽  
Author(s):  
P M Kimball ◽  
M G Brattain ◽  
W E White
Keyword(s):  
Molecular Weight ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Parental Cell ◽  
Carcinoma Cell Line ◽  
Colonic Carcinoma ◽  
Lower Molecular Weight ◽  
Parental Cell Line ◽  
Colonic Carcinoma Cell Line

A sub-line with increased metastatic ability was previously isolated from an established human colonic carcinoma cell line [Kimball & Brattain (1980) Cancer Res. 40, 1574-1579]. The separation and characterization of the isoenzymes of N-acetyl-beta-D-hexosaminidase from each cell line are reported. The parental cell line contained A and B isoenzymes. The sub-line lacked the A-isoenzyme activity and contained an atypical B isoenzyme that was thermolabile, susceptible to alkylation and of lower molecular weight.

scholarly journals Suppression of IgE antibody production in SJL mice. III. Characterization of a suppressor substance extracted from normal SJL spleen cells.

1977 ◽  
Vol 145 (6) ◽  
pp. 1501-1510 ◽  
Author(s):  
N Watanabe ◽  
Z Ovary
Keyword(s):  
Antibody Production ◽  
High Titer ◽  
Gel Filtration ◽  
Keyhole Limpet Hemocyanin ◽  
Cell Extract ◽  
Nippostrongylus Brasiliensis ◽  
Spleen Cells ◽  
Ige Antibody ◽  
Third Stage

SJL mice were immunized with 1 microng dinitrophenylated keyhole limpet hemocyanin in 1 mg Al(OH)3. The mice were infected 21 days later with 750 third stage larvae of Nippostrongylus brasiliensis. On day 35, 14 days after infection, they were injected with 1 microng DNP-N, brasiliensis extract (Nb) in 1 mg Al(OH)3. In order to obtain high titer and persistent anti-DNP IgE antibody the mice were irradiated (540 R) 1 day after injection of DNP-Nb. Suppression of anti-DNP IgE antibody production was induced by spleen cells from normal SJL mice. Suppression of IgE antibody response is also obtained by an extract from normal SJL spleen cells. The suppressor substance from normal SJL spleen cell extract is a heat-labile protein, and is not absorbed by anti-mouse immunoglobulin. The mol wt of this substance is larger than 300,000 daltons as determined by gel filtration on Sephadex G-200, but after ultracentifugation, the supernate still has suppressive activity on IgE antibody production.

Characterization of a high molecular weight protective antigen ofPlasmodium yoelli

Parasitology ◽  
1984 ◽  
Vol 88 (2) ◽  
pp. 211-219 ◽  
Author(s):  
A. A. Holder ◽  
R. R. Freeman
Keyword(s):  
Molecular Weight ◽  
Monoclonal Antibodies ◽  
Specific Antibody ◽  
Protective Antigen ◽  
Peptide Mapping ◽  
Plasmodium Yoelii ◽  
Lower Molecular Weight ◽  
Large Protein ◽  
Lower Molecular Weight Species

SUMMARYA 230000 molecular weightPlasmodium yoeliiprotective antigen was characterized. Two monoclonal antibodies againstP. yoeliiimmunoprecipitated the 230000 mol. wt protein and a number of lower molecular weight polypeptides. These polypeptides were shown by peptide mapping and specific antibody binding to be fragments of the large protein. Iodination experiments suggested that the lower molecular weight species may be present on the surface of the merozoite. The protein was found not to be glycosylated. By serology, related antigens were shown to be associated with blood-stage schizonts ofP. vinckeisubspp.,P. chabaudisubspp. andP. falciparum.

Characterization of lower molecular weight artifact bands of recombinant monoclonal IgG1 antibodies on non-reducing SDS-PAGE

2007 ◽  
Vol 29 (11) ◽  
pp. 1611-1622 ◽  
Author(s):  
Hongcheng Liu ◽  
Georgeen Gaza-Bulseco ◽  
Chris Chumsae ◽  
Abigail Newby-Kew
Keyword(s):  
Molecular Weight ◽  
Lower Molecular Weight ◽  
Sds Page

Synthesis and Characterization of Sugar-Bearing Chitosan Derivatives as a Scaffold for Nitric Oxide Release

2010 ◽  
Vol 160-162 ◽  
pp. 1083-1089
Author(s):  
Yan Sun ◽  
Yong Liu
Keyword(s):  
Molecular Weight ◽  
Gluconic Acid ◽  
Lower Molecular Weight ◽  
No Donor ◽  
Chitosan Derivatives ◽  
Nitric Oxide Release ◽  
High Storage Capacity ◽  
High Storage ◽  
Donor Species

These new synthesized diazeniumdiolates obtained from D-gluconic acid-bearing chitosan derivatives (SBC) with different molecular weight (280 kDa and 880 KDa) and D-gluconic acid-bearing O-carboxymethyl chitosan derivatives (880 KDa) (SBCS) were used as the NO donor species. Compared with SBC with much higher molecular weight (880 KDa), the SBC with the lower molecular weight (280 kDa) exhibited a high storage capacity for NO (up to 408 nmol NO/mg), greatly increasing the “payload” of released NO with the molecular weight of SBC decreasing. The NO release durations (0.326 h) observed for secondary amine on the SBC with the lower molecular weight (280 kDa) were slightly higher than that of SBC diazeniumliolates with the higher molecular weight (880 kDa), which was only 0.294h. The release duration of SBCS-NO adducts (0.381h) was obviously longer than that of SBC-NO adducts, when they have the same molecular weight (880 kDa).

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