Gene transcription repression inClostridium beijerinckiiusing CRISPR-dCas9

2016 ◽  
Vol 113 (12) ◽  
pp. 2739-2743 ◽  
Author(s):  
Yi Wang ◽  
Zhong-Tian Zhang ◽  
Seung-Oh Seo ◽  
Patrick Lynn ◽  
Ting Lu ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Transcription Repression

Activator protein-1 is necessary for angiotensin-II stimulation of human adrenocorticotropin receptor gene transcription

2001 ◽  
Vol 268 (6) ◽  
pp. 1802-1810
Author(s):  
Danielle Naville ◽  
Estelle Bordet ◽  
Marie-Claude Berthelon ◽  
Philippe Durand ◽  
Martine Begeot
Keyword(s):  
Angiotensin Ii ◽  
Gene Transcription ◽  
Receptor Gene ◽  
Activator Protein 1 ◽  
Activator Protein ◽  
Stimulation Of

Regulation of neuronal cholecystokinin gene transcription

2001 ◽  
Vol 61 (7) ◽  
pp. 61-67 ◽  
Author(s):  
Thomas V. O. Hansen ◽  
Finn C. Nielsen
Keyword(s):  
Gene Transcription

Q-mediated late gene transcription of bacteriophage λ: RNA start point and RNase III processing sites in vivo

Virology ◽  
1988 ◽  
Vol 167 (2) ◽  
pp. 568-577 ◽  
Author(s):  
D DANIELS ◽  
M SUBBARAO ◽  
F BLATTNER ◽  
H LOZERON
Keyword(s):  
Gene Transcription ◽  
Late Gene ◽  
Bacteriophage Λ ◽  
Rnase Iii

GENE ACTIVATION IN MAMMARY CELLS

1972 ◽  
Vol 71 (2_Suppla) ◽  
pp. S346-S368 ◽  
Author(s):  
Roger W. Turkington ◽  
Nobuyuki Kadohama
Keyword(s):  
Cell Differentiation ◽  
Gene Transcription ◽  
Hormonal Regulation ◽  
Gene Activation ◽  
Milk Proteins ◽  
Mouse Mammary Gland ◽  
Nuclear Proteins ◽  
Mammary Cells ◽  
Alveolar Cells ◽  
Mammary Cell

ABSTRACT Hormonal activation of gene transcription has been studied in a model system, the mouse mammary gland in organ culture. Transcriptive activity is stimulated in mammary stem cells by insulin, and in mammary alveolar cells by prolactin and insulin. Studies on the template requirement for expression of the genes for milk proteins demonstrate that DNA methylation has an obligatory dependence upon DNA synthesis, but is otherwise independent from hormonal regulation of mammary cell differentiation. Incorporation of 5-bromo-2′deoxyuridine into DNA selectively inhibits expression of the genes for specific milk proteins. Undifferentiated mammary cells activate the synthesis of specific acidic nuclear proteins when stimulated by insulin. Several of these induced acidic nuclear proteins are undetectable in unstimulated undifferentiated cells, but appear to be characteristic components of the nuclei of differentiated cells. These results indicate that mammary cell differentiation is associated with a change in acidic nuclear proteins, and they provide evidence to support the concept that acidic nuclear proteins may be involved in the regulation of gene transcription and of mammary cell differentiation.

Spatio-temporal analysis of prolactin gene transcription dynamics reveals short range co-ordination of lactotroph transcription activity in pituitary tissue

2014 ◽  
Author(s):  
Karen Featherstone ◽  
Kirsty Hey ◽  
Hiroshi Momiji ◽  
Anne McNamara ◽  
Amanda Patist ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Short Range ◽  
Temporal Analysis ◽  
Transcription Activity ◽  
Pituitary Tissue ◽  
Prolactin Gene ◽  
Spatio Temporal

Analysis of ∼700 Human Tissue Samples Identifies microRNAs That Are Associated with, Predictive of, and Necessary for Insulin Gene Transcription

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 2160-P
Author(s):  
ANAND HARDIKAR ◽  
WILSON WONG ◽  
MUGDHA JOGLEKAR ◽  
LOUISE T. DALGAARD ◽  
ALICIA JENKINS ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Human Tissue ◽  
Insulin Gene ◽  
Tissue Samples ◽  
Insulin Gene Transcription

PLASMACYTOID DENDRITIC CELLS FUNCTION IN HIV INFECTION

2008 ◽  
Vol 31 (4) ◽  
pp. 13
Author(s):  
Martin Hyrcza ◽  
Mario Ostrowski ◽  
Sandy Der
Keyword(s):  
Dendritic Cells ◽  
Influenza Virus ◽  
Hiv Infection ◽  
Gene Transcription ◽  
Sendai Virus ◽  
Plasmacytoid Dendritic Cells ◽  
Type I Interferons ◽  
Interferon Response ◽  
Type I ◽  
Type I Ifn

Plasmacytoid dendritic cells (pDCs) are innate immune cells able to produce large quantities of type I interferons (IFN) when activated. Human immunodeficiency virus (HIV)-infected patients show generalized immune dysfunction characterized in part by chronic interferon response. In this study we investigated the role of dendritic cells inactivating and maintaining this response. Specifically we compared the IFN geneactivity in pDCs in response to several viruses and TLR agonists. We hypothesized that 1) the pattern of IFN gene transcription would differ in pDCs treated with HIV than with other agents, and 2) that pDCs from patients from different stages of disease would respond differently to the stimulations. To test these hypotheses, we obtained pDCs from 15 HIV-infected and uninfected individuals and treated freshly isolated pDCs with either HIV (BAL strain), influenza virus (A/PR/8/34), Sendai virus (Cantell strain), TLR7 agonist(imiquimod), or TLR9 agonist (CpG-ODN) for 6h. Type I IFN gene transcription was monitored by real time qPCRfor IFNA1, A2, A5, A6, A8,A17, B1, and E1, and cytokine levels were assayed by Cytometric Bead Arrays forTNF?, IL6, IL8, IL10, IL1?, and IL12p70. pDC function as determined by these two assays showed no difference between HIV-infected and uninfected patients or between patients with early or chronic infection. Specifically, HIV did notinduce type I IFN gene expression, whereas influenza virus, Sendai virus and imiquimod did. Similarly, HIV failed to induce any cytokine release from pDCs in contrast to influenza virus, Sendai virus and imiquimod, which stimulatedrelease of TNF?, IL6, or IL8. Together these results suggest that the reaction of pDCs to HIV virus is quantitatively different from the response to agents such as virus, Sendai virus, and imiquimod. In addition, pDCs from HIV-infected persons have responses similar to pDCs from uninfected donors, suggesting, that the DC function may not be affected by HIV infection.

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